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1 2002

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Estimation of scattering volume in short distance reflectance measurements by Monte Carlo modelling

Liebert A., Terajewicz N., Maniewski R., Nilsson G., De Mul F.F.M.

Optica Applicata

2002

Vol. 32, nr 4

709-720

The depth of light penetration is an interesting issue in various tissue optics experiments. The measurements of remitted light distribution was shown to allow for estimation of optical properties. However, in case of measurements with a short distance between the emitter and the detector, the diffusion equation fails, and the proposed methods cannot be applied. The aim of this study was to develop a simple method for estimation of the light penetration depth for short-distance measurements of remitted light. The idea is based on the analysis of the diffusely back-scattered light distribution adjacent to the emitter for the assessment of the scattering volume. The Monte Carlo method was used for theoretical estimation of the relationship between the light distribution around emitting fiber and the depth of light scattering volume. Two series of Monte Carlo simulations were performed: the first one with limited and the second one with unlimited scattering volume. In both series the values of absorption and scattering coefficients were altered within ranges typical of human tissues. The results of this study show that: the diffuse reflectance is strongly dependent on the absorption and scattering properties of the tissue and it is possible to estimate the depth of the scattering volume by use of the short distance profile of diffusely back-scattered light as measured at the surface of the tissue.