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Wykorzystanie chromatografii membranowej do odzyskiwania białek aktywnych biologicznie z odpadów przemysłu skrobiowego

Markiewicz M., Przewodowska A., Przewodowski W., Stochła W.

Rocznik Ochrona Środowiska

2015

Tom 17, cz. 2

1699--1714

Starch industry generates enormous amount of wastes which contains several valuable components such as minerals, proteins and amino acids. In the past those wastes were released to the environment in unchanged form or used as fertilizers or animal feed. However, economical and ecological functionality of those applications was minor and rather a result of lack of better options than real needs. Currently, due to the urgent necessity of environment pollution prevention as well as for economic reasons, research concerning development of new methods of recovery, bioconversion and utilization of valuable substances present in wastes is being undertaken. This publication aims to describe strategies of starch industry wastes conversion, mainly potato soap, into useful products. Historical, presently used and advanced biotechnological methods were described. In addition, an economical and ecological evaluation of methods was performed. Ion-Exchange chromatography can be an economically profitable technology for the isolation and purification of different native potato proteins. Using membrane adsorption instead of classical chromatography has many advantages. Further availability of filter modules with different shapes and capacities enables the efficient isolation of proteins on an industrial scale. The application of ion-exchange modules for active recovery of proteins from potato juice is confirmed by research. In recent years, Sartorius Stedim Biotech company has designed new modules with polymer spacers and different channel sizes (MA-IEX-modules) enabling processing of crude, particle-containing solutions using a tangential flowthrough the membranes. The great advantage of this solution is the possibility of omitting the first steps of the purification, what saves time and money. The usefulness of such units was confirmed by isolation of valuable potato proteins: patatin and low molecular weight from potato fruit juice from starch factory. In this study a preliminary researches of the possibility of using the membrane ion exchange chromatography as a technique for recovery of biologically active protein from waste material which is potato pulp is also presented.