Wyniki wyszukiwania - BazTech

1

Computer-aided image analysis for microcapsules' quality assessment

Przytulska M., Kulikowski J. L., Lewińska D., Grzeczkowicz M., Kupikowska-Stobba B.

Biocybernetics and Biomedical Engineering

|

2015

|

Vol. 35, no. 4

342--350

EN

Application of computer-aided image analysis to quality assessment of inner structure of alginate-polyethersulfone microcapsule is presented. The microcapsules are provided for analysis in the form of optical microscope images of their cross-sections. A specialized computer program APEK makes it possible to measure and calculate a set of morphological parameters describing microcapsules' outer and inner size and shape, as well as the thickness of a polyethersulfone membrane which covers hydrogel microcapsule core. It is described a method of thickness profile of microcapsule's membrane measuring based on skeleton line. Calculations are illustrated by an example. Suggestions concerning future works have been formulated.

2

Studies on the structure of semi-permeable membranes by means of SEM problems and potential sources of errors

Chwojnowski A., Wojciechowski C., Lewińska D., Łukowska E., Nowak J., Kupikowska-Stobba B., Grzeczkowicz M.

Biocybernetics and Biomedical Engineering

|

2012

|

Vol. 32, no. 1

51-64

EN

The effect of sputtering with a conductor of the semi-permeable membranes surface on SEM pictures obtained is presented. On the example of photomicrographs of several different types of semi-permeable membranes, changes in the appearance of various membrane surfaces, uncovered and sputtered with thicker and thicker layers of the conductor are presented. It has been shown, how essential differences in the appearance of the studied material can be caused by the deposited conductor. It has been shown what errors in the interpretation of SEM images can be caused by applying the sputtered conductor layer with a thickness insufficient to the structure and properties of the studied material. Necessity of minimizing the layer thickness of the sputtered conductor and experimental determination of the sputtered layer thickness was found. Appropriateness of taking the pictures in the mode without sputtering and necessity of comparing the pictures with and without sputtering have been suggested. The useful way of carrying out magnifications' of membranes made of polymers of low melting points has been also presented.

3

Culture of human autologous chondrocytes on polysulphonic membrane - preliminary studies

Płończak M., Czubak J., Chwojnowski A., Kupikowska-Stobba B.

Biocybernetics and Biomedical Engineering

|

2012

|

Vol. 32, no. 3

63-67

EN

This work investigated an effective method of isolation and culture of human autologous chondrocytes placed on a polysulphonic membrane. The cartilage was taken from the hip joint of 78 years old woman who underwent total hip ar throplasty due to idiopatic arthrosis and from the knee of 46 years old man with cartilage lesion from non-weight bear area. The cells were released from the matrix in the course of enzymatic digestion. The isolated cells were placed with parts of polysulphonic membrane in the same culture flask and incubated. Due to evaluation the weight of tissue grown on the polysulphonic membrane the elementary analysis was performed. The elementary analysis of the polysulphonic membrane slices after ten weeks of the culture revealed higher concentration of the tissue on one part of the membrane in case of the older woman - 0.726 mg of protein per 1 mg of the membrane then in case of man - 0.513 mg per 1 mg. The established method of isolation and culture of chondrocytes is effective enough to provide a sufficient number of cells that can be used as a transplant.