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2 2013

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An ammonia sensor based on in situ-synthesized polyaniline nanostructures

Dobroczyńska J I, Grabas K

Materials Science Poland

2013

Vol. 31, No. 1

95--100

Nowadays, a good deal of research is focusing on the development of new analytical procedures for detection of hazardous agents, such as bacteria, harmful pollutants, toxic vapors, and many others. The majority of the research is aimed at developing new sensors and analytical equipment. This paper demonstrates a simple construction of an ammonia sensor based on commercially available electronic connectors with active elements from polyaniline. The main goal of this research is to present the simplicity of sensor manufacturing, without the need for advanced apparatus such as electropolymerization or electrospinning set-up. The active element of the proposed sensor is composed of polyaniline synthesized in situ on the surface of conductive glue. The layer of the conductive glue is a key element in this sensor as it protects the metallic pin against destruction during in situ synthesis of polyaniline (acidic and oxidizing conditions).

Application of an aptamer and a reagent based on gold nanoparticles for detection of Escherichia coli

Pięta P. P., Szczerba A., Dobroczyńska J. I., Grabas K.

Environment Protection Engineering

2013

Vol. 39, nr 4

125--133

Plates functionalized with an Escherichia coll binding aptamer and a reagent based on gold nanoparticles were used for the detection of bacterial cells. Bacteria suspended in samples were caught by specific aptamers immobilized on a plastic plate whereas the gold nanoparticle reagent is an indicator reagent for visualization of the test result. Changes in colour of the gold nanoparticle reagent caused by gold nanoparticle agglomeration were observed with the naked eye. The test was designed for the detection of Escherichia coli in water suspensions.