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Surface functionalization of poly(L-lactide-co-glycolide) membranes with amphiphilic poly(2-oxazoline) for guided tissue regeneration and treatment of bone tissue defects

Tryba A. M., Krok-Borkowicz M., Paluszkiewicz C., Pamuła E.

Engineering of Biomaterials

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2018

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Vol. 21, no. 147

16--20

EN

The main challenge of this research was to functionalize the surface of poly(L-lactide-co-glycolide) (PLGA) membranes with amphiphilic poly(2-oxazoline) (POx) in order to change PLGA chemical state and properties. Poly(2-oxazolines) are very powerful polymers, which thanks to active pendant groups can be easily functionalized with biologically active molecules or peptides. The membranes were prepared by dissolving PLGA, POx, and poly(ethylene glycol) (PEG, 1000 Da) in methylene chloride (DCM), followed by PEG leaching. POx molecules were preferentially adsorbed at the interface PLGA-POx-PEG thanks to affinity to both hydrophilic (PEG) and hydrophobic (PLGA) chains. The properties of the membranes were characterized with Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM) and wettability tests. Cytocompatibility of the materials in contact with osteoblast-like MG-63 cells was studied by evaluation of cell viability (Alamar-Blue test), live/dead and phalloidin/DAPI staining. The results show that the presence of POx influenced topography of the PLGA membranes, but did not have an impact on their wettability. All membranes were fo-und cytocompatible with model osteoblasts. Presence of POx resulted in better cell adhesion as shown by microscopic studies after fluorescence staining for nuclei and cytoskeleton actin filaments. In summary, one-step phase separation process between PLGA, PEG, and POx, dissolved in DCM followed by drying and PEG leaching resulted in cytocompatible PLGA membranes with immobilised POx, which might be considered for guided tissue regeneration technique in periodontology and in bone tissue engineering.

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Modification of microparticles’ microstructure with carbon dioxide for application as cell carriers in modular tissue engineering

Mielan B., Krok-Borkowicz M., Pamuła E.

Engineering of Biomaterials

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2018

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Vol. 21, no. 148

102

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Biomimetic in situ nucleation of calcium phosphate/Ag coating on bioinert ceramic

Schickle K., Desante G., Goldschmidt G. M., Zybała R., Krok-Borkowicz M., Pamuła E., Telle R.

Engineering of Biomaterials

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2018

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Vol. 21, no. 148

27

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Modification of PLGA microspheres’ microstructure for application as cell carriers in modular tissue engineering

Mielan B., Krok-Borkowicz M., Pielichowska K., Pamuła E.

Engineering of Biomaterials

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2017

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Vol. 20, no. 140

7--11

EN

Microspheres (MS) made of resorbable polymer have been proposed as a cell growth support. They may be assembled to form cell constructs or be suspended in hydrogels allowing injection into injury location. High relative surface area of MS provides more efficient cell culture environment than traditional culture on flat substrates (multiwell plates, Petri dishes). In addition, MS structure, topography and surface chemistry can be modified to promote cell adhesion and proliferation. The aim of this study was to obtain resorbable poly(L-lactide-co-glycolide) (PLGA) MS and to modify their properties by changing manufacturing conditions of the oil-in-water emulsification to better control structural and microstructural properties of MS and their biological performance. To this end, water phase was modified by addition of NaCl to change ionic strength, while oil phase by addition of polyethylene glycol (PEG). Microstructural and thermal properties were assessed. Cytocompatibility tests and cell cultures with MG-63 cells were conducted to verify potential relevance of MS as cell carriers. The results showed that it is possible to obtain cytocompatible MS by oil-in-water emulsification method and to control diameter, porosity and crystallinity of MS with the use of additives to oil and/or water phases without negative changes in MS cytocompatibility. The results prove that modification of both phases make it possible to produce MS with desired/controllable properties like surface topography, porosity and crystallinity.

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Modification of microspheres’ microstructure for application as cell carriers in modular tissue engineering

Mielan B., Krok-Borkowicz M., Pielichowska K., Pamuła E.

Engineering of Biomaterials

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2017

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Vol. 20, no. 143 spec. iss.

72

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Hybrid, bioactive coatings formed on titanium alloys surface

Kazek-Kęsik A., Nosol A., Płonka J., Krok-Borkowicz M., Pamuła E., Simka W.

Engineering of Biomaterials

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2017

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Vol. 20, no. 143 spec. iss.

41

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The effect of titanium dioxide addition on physical and biological properties of Na2O-B2O3-P2O5 and CaO-Na2O-P2O5 glasses

Kalwasińska O., Gajc M., Kłos A., Orliński K., Pawlak D. A., Krok-Borkowicz M., Rumian Ł., Pietryga K., Reczyńska K., Pamuła E.

Engineering of Biomaterials

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2016

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Vol. 19, no. 134

2--7

EN

Two types of phosphate glasses 50Na2O-20B2O3-30P2O5 (NBP) and 30CaO-20Na2O-50P2O5 (CNP) with different content of TiO2 (0, 3 and 5 mol%) have been prepared by melt-quenching process. TiO2 was added to increase glass network stability. Physical properties of glasses were investigated by density measurements, differential scanning calorimetry and degradation in phosphate buffered saline (PBS). Biological performance of glasses in a direct contact with osteoblast-like MG-63 cells was analysed with the use of resazurin test and live-dead staining. The results show that TiO2 addition increased density, glass transition temperature (Tg) and melting temperature (Tm) of both types of glasses. In the case of NBP glasses presence of TiO2 resulted in their fast degradation in PBS and acidification of cell culture medium. As a consequence such glasses did not support cell adhesion and growth, but they can be considered for e.g. drug delivery systems. On the other hand addition of TiO2 to CNP glasses resulted in enhanced cell adhesion and viability. Particularly positive results were found for CNP glass containing 5% TiO2, so it can be a good candidate as a scaffold material for bone tissue engineering.

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Novel injectable, self-gelling hydrogel-microparticle composites for bone regeneration consisting of gellan gum and calcium and magnesium carbonate microparticles

Douglas T. E. L., Łapa A., Reczyńska K., Krok-Borkowicz M., Pietryga K., Samal S. K., Schaubroeck D., Boone M., Voort P. van der, Schamphelaere K. de, Stevens C. V., Bliznuk V., Parakhonskiy B. V., Cnudde V., Pamuła E., Skirtach A. G.

Engineering of Biomaterials

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2016

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Vol. 19, no. 138 spec. iss.

111

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Enrichment of thermosensitive chitosan hydrogels with glycerol and alkaline phosphatase for bone tissue engineering applications

Douglas T. E. L., Krok-Borkowicz M., Macuda A., Pietryga K., Pamuła E.

Acta of Bioengineering and Biomechanics

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2016

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Vol. 18, no. 2

51--57

EN

Thermosensitive injectable chitosan hydrogels can be formed by neutralization of acidic chitosan solutions with sodium betaglycerophosphate (Na-β-GP) coupled with increasing temperature to body temperature. Such hydrogels have been considered for applications in bone regeneration. In this study, chitosan hydrogels were enriched with glycerol and the enzyme alkaline phosphatase (ALP) with a view to improving their suitability as materials for bone tissue engineering. Mineral formation was confirmed by infrared spectroscopy (FTIR) and increases in the mass fraction of the hydrogel not consisting of water. Incorporation of ALP in hydrogels followed by incubation in a solution containing calcium ions and glycerophosphate, a substrate for ALP, led to formation of calcium phosphate within the hydrogel. MG-63 osteoblast-like cells were cultivated in eluates from hydrogels containing ALP and without ALP at different dilutions and directly on the hydrogel samples. Hydrogels containing ALP exhibited superior cytocompatibility to ALP-free hydrogels. These results pave the way for the use of glycerol- and ALP-enriched hydrogels in bone regeneration.