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1 Acta Chromatographica

1 2002

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Quantification of lutein in dietary supplements by reversed-phase high-performance thin-layer chromatography with visible-mode densitometry

Sechrist J., Pachuski J., Sherma J.

Acta Chromatographica

2002

No. 12

151-158

A quantitative method, using chemically bonded C18 silica gel HPTLC plates, automated band-wise sample application, and automated visible-mode densitometry, has been developed for the determination of the carotenoid pigment lutein in nutrition supplements consumed to reduce the risk of macular degeneration. Three products containing 20 mg or 6 mg of lutein as the free alcohol or dipalmitate ester plus other ingredients were analyzed. Comparison with a lutein reference standard revealed that recoveries ranged from 112 to 247% relative to the stated label value. Accuracy was validated by analysis of a spiked blank and a second certified lutein standard, and precision by performing replicate analysis. Accuracy was found to be within 0.1% and 0.4% of theoretical values, and precision was 1.59% relative standard deviation.