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1 Engineering of Biomaterials

1 2010

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Human osteoblast-like MG 63 cells in cultures on nanofibrous PLGA membranes loaded with nanodiamonds

Parizek M., Douglas T. E. L., Kromka A., Renzing A., Voss E., Brady M. A., Warnke P. H., Bacakova L.

Engineering of Biomaterials

2010

Vol. 13, no. 94

11--13

Using an electrospinning technique, we constructed composite nanofibrous membranes containing a copolymer of L-lactide and glycolide (PLGA, ratio 85:15) and 33 wt% of nanodiamond particles. he number of initially adhering human osteoblast-like MG 63 cells on day 1 after seeding, their spreading and subsequent growth were similar on both types of membranes. However, higher cell numbers on day 3 and 7 after seeding and a larger cell spreading area were found in the cells in the control polystyrene cell culture dishes. Nevertheless, the composite PLGA-ND membranes provided relatively good support for colonization with bone-derived cells; thus this material is promising for bone tissue engineering.