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EN
The performance of horizontal subsurface flow constructed wetland (HFCW) for rural domestic sewage treatment has been evaluated. The system was built as a tertiary treatment after the biological processes to improve the effluent wastewater quality. The HFCW was operated in three phases under different hydraulic loading rates (HLRs), and with three kinds of aquatic plants i.e., water spinach, Chinese celery and cress. The vegetation growth parameters such as plant height, fresh and dry weights were monitored and analyzed. The influent and effluent concentrations of the chemical oxygen demand (COD), ammonium nitrogen (NH4+-N), total nitrogen (TN) and total phosphorus (TP) were measured. The average removal efficiencies at the first phase were 52.9%, 64.7%, 58.2% and 72.8%, and it reduced to 48.6%, 52.2%, 44.04% and 64.4% in the second phase for COD, NH4+-N, TN and TP, respectively. In the third phase, the HFCW system showed the following mean removal efficiencies: 51.2%, 74.2%, 58.5% and 80.9%. The results revealed that the removal efficiencies increased with decrease in the HLR and increased temperatures. The findings confirmed that the horizontal flow constructed wet-land is more convenient for the rural wastewater treatment with efficient nutrient removal.
EN
A new strategy of enrichment of polyphosphate accumulating organisms (PAO) and denitrifying polyphosphate accumulating organisms (DPAO) at low temperature ranging from 8 °C to 11 °C was demonstrated through two lab-scale reactors operated in sequential anaerobic-aerobic (AO) or anaerobic-anoxic (AA) conditions. It was found that the AO reactor is able to achieve a good phosphorus removal performance after 40 days of operation, while a similar stable phosphorus removal can be obtained in the AA reactor after 80 days. This result suggests that the enrichment of PAO was easier than that of DP AO at low temperature. Through switching batch tests, when DPAO is exposed to aerobic conditions, it can immediately exhibit a good phosphorus removal similar to that under anoxic conditions, while PAO can only present poor phosphorus removal when exposed to anoxic conditions, suggesting that two different types of Accumulibacter were enriched both in AA and AO reactors. Microbial analysis with fluorescence in situ hybridization (FISH) and DAPI (4',6-diamidino-2 -phenylindole) staining revealed that Accumulibacter was dominant both in the two reactors, accounting for 61.6% and 79.3% of all bacteria in AA and AO reactors, respectively. Although the different amount of Accumulibacter was enriched in the two reactors, the similar microbial morphologies were observed by using scanning electron micrograph (SEM), both presenting long-rod morphology. This kind of Accumulibacter may display affinities for sodium acetate used as the carbon sources here. This strategy proposed in this study was shown to be effective in achieving a very high enrichment of Accumulibacter at low temperature by linking chemical analysis with microbial observation.
EN
An anaerobic-anoxic/oxic (AA/O) five-tank biological process called five-step continuous flow activated sludge process (FSCFASP) was developed to force the oscillation of organic matter and nutrient concentrations in process reactors. The run scheme and schematic diagram of FSCFASP was described. The run cycle was divided into two symmetrical half cycles with eight periods during each cycle. The duration of each period was established according to biological process requirements. The optimal running times of the periods were 90, 60, 60, 30, 90, 60,6 0 and 30 min at the HRT of 16 h, air/water ratio of 35% and SRT of 21 day at the temperature range of 19-23 °C. The optimized system achieved 88.09±1.43%, 90.33±2.9%, 68.83±5.34% and 87.67±2.9% of the chemical oxygen demand, NH4+-N, TN, and TP removal efficiencies, respectively, during a 11-month operation with the effluent meeting the Chinese sewage discharge standard GB18918-2002 (level A). Simultaneous nitrification and denitrification phenomena were observed in the tank one which is important to reduce the quantity of aeration and the duration of a next anoxic state. Compared with other existing technologies, this system achieved high nitrogen and phosphorus removal without equipment of sludge and mixed liquor return. Thus, it is effective for reducing energy consumption.
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