Wyniki wyszukiwania - BazTech

Ograniczanie wyników

2 Oceanological and Hydrobiological Studies

2 2021

Znaleziono wyników: 2

Liczba wyników na stronie

Wyniki wyszukiwania

Sortuj według:

Ogranicz wyniki do:

Inter-clutch egg differences and androgenesis in rainbow trout (Oncorhynchus mykiss, Walbaum 1792)

Polonis Marcin, Błaszczyk Agata, Jagiełło Krzysztof, Panasiak Ligia, Dobosz Stefan, Ocalewicz Konrad

Oceanological and Hydrobiological Studies

2021

Vol. 50, No. 2

160--168

Ionizing radiation (IR) is applied to inactivate the nuclear genome in rainbow trout eggs during induced androgenetic development. However, IR-generated reactive oxygen species (ROS) may affect developmental potential of eggs and reduce the effectiveness of androgenesis. To verify this assumption, androgenetic development of rainbow trout was induced in eggs irradiated with 350 Gy of X-rays. Survival rates, pH of the ovarian fluid and activity of antioxidant enzymes, including SOD, CAT and GPx, were examined in non-irradiated and irradiated eggs originating from four females. Survival rates of androgenetic embryos developing in eggs produced by different females varied from 1% to 57% and these inter-clutch differences were significant. Eggs from female F4, which showed the highest developmental competence for androgenesis, also showed increased activities of SOD, CAT and GPx enzymes. The pH value of the ovarian fluid of each female was over 8 before and after irradiation, therefore it seems that radiation did not affect the ovarian fluid pH. Considering the above-mentioned inter-clutch differences, a strong maternal effect on the effectiveness of androgenesis can be assumed. Eggs with increased activity of antioxidant enzymes before irradiation should be expected to show increased developmental competence for androgenesis.

Incidence of skeletal deformities in induced triploid rainbow trout Oncorhynchus mykiss (Walbaum, 1792)

Jagiełło Krzysztof, Polonis Marcin, Ocalewicz Konrad

Oceanological and Hydrobiological Studies

2021

Vol. 50, No. 2

150--159

Due to the cytogenetic incompatibility, triploid fish are usually infertile and are not affected by a decline in growth, survival and meat quality, which accompanies the process of sexual maturation in diploid specimens. Thus, artificial triploidization has been proposed for fish production in the case of species with early sexual maturation, such as rainbow trout. However, the use of this technique is limited by increased ratios of skeletal deformities observed in triploid specimens. The main objective of this research was to compare the proportion and variety of body abnormalities in diploid and triploid 14-month-old rainbow trout from commercial stocks, using external body shape examination, radiography and whole-mount skeletal staining. Individuals with externally observed body deformities (scoliosis, humpback, shortened tail and jaw deformities) accounted for 0.45% of the diploid stock and 3.83% of the triploid stock. X-rays and whole-mount skeletal staining of deformed individuals showed spine deformities, including compressions and fusions of vertebrae. Abnormalities observed in diploid and triploid rainbow trout examined during this study were non-lethal, however, they may negatively affect the condition of fish. Fish with skeletal deformities are not aesthetically pleasing, thus an increased ratio of such deformations in fish produced for commercial purposes may result in real economic losses.