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1

Simple and Combined Pretreatment of a Mixture of Forestry and Aromatic-Medicinal Plant Waste by Chemical, Physical and Enzymatic Methods

Bendaoud Ahmed, Belkhiri Abdelkhalek, Maai Mohamed, Moubchir Tarik, Hmamou Anouar, Tlemcani Sara, Eloutassi Noureddine, Lahkimi Amal

Journal of Ecological Engineering

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2023

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Vol. 24, nr 4

376--383

EN

Forestry waste (FW) extracted parts ofmedicinal-aromatic plant waste (EPW) and unused parts (UPW) are considered potential resources for energy recovery (their heating value of approximately 19 MJ/kg).In order to valorize lignocellulosic biomass, a pretreatment process is required to hydrolyze the recalcitrant lignocellulosic complex into fermentable simple sugars. The aim of this study is to determine the best method of pretreatment that takes into account treatment time, efficiency, and environmental friendliness. The mixture of FW, EPW, and UPW was treated by simple and combined treatment using different methods like acid sulfuric (Ac), steam explosion (SE), and enzymatic (E) (cellulase and hemicellulase).The results showed that the combined and simple Ac treatments are the mostefficient compared with SE and E treatments in the hydrolysis of polysaccharide of cellulose with a rate respectively of 90.5% and 77.6% and hemicellulose with a rate respectively of 80.63% and 87.14%. In addition, both of the preceding methods release an important rate of total phenolic compounds. Combined treatment demands high time but is friendly (approximately 1 day), and Ac treatment is less time-consuming (about 25 min) but harmful to the environment and causes the corrosion of equipment.In conclusion, combined treatment can be the best method and the high time required can be reduced with the progress of the research.

2

Impact of Water Stress and Temperature on Metabolites and Essential Oil of Rosmarinus officinalis (Phytochemical Screening, Extraction, and Gas Chromatography)

Laftouhi Abdelouahid, Eloutassi Noureddine, Drioua Soufiane, Ech-Chihbi Elhachmia, Rais Zakia, Abdellaoui Abdelfattah, Taleb Abdslam, Beniken Mustapha, Taleb Mustapha

Journal of Ecological Engineering

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2023

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Vol. 24, nr 5

237--248

EN

Currently, climate change is disrupting life on Earth by causing imbalances in the biosphere. This work aimed to evaluate the impact of climate change on the content of primary and secondary metabolites and the yield of essential oil of Rosmarinus officinalis. Thus, the results of the conducted experiment show that the content of primary metabolites decreased with increasing temperature and decreasing precipitation along the experiment(proteins from 7.61% to 7.14%, carbohydrates from 6.92% to 5.64%, fats from 1.48% to 1.29% and dietary fiber from 4.96% to 4.22% and mineral composition: Ca from 7.67% to 5.98%, Mg from 8.61% to 7.01%, Fe from 7.53% to 7.21% and Mn from 6.85% to 3.97%), and the content of secondary metabolites increased in the second year when increasing the temperature by 5 °C and water stress by 50% (coumarin from 6.59% to 10.99%, saponins from 7.15% to 8.46%, tannin from 3.92% to 5.95%, alkaloids from 6.69% to 15.62% and flavonoid from 8.02% to 15.75%),but in the fourth year when the temperature continued to increase and water stress was 75% the content of secondary metabolites decreased (coumarin from 10.99% to 8.27%, saponins from 8.46% to 7.87%, tannin from 5.95% to 4.85%, alkaloids from 15.62% to 10.68% and flavonoid from 15.75% to 11.36%)and the same results were obtained for the yield of essential oil which increased in the second year and decreased in the fourth year. This GC analysis of the three essential oil samples shows that the majority of compounds in the three essential oils studied are cineole (S1:45.98%, S2:55.36%, S3:43.08%) followed by camphor (S1:17.44%, S2:21.44%, S3:21.56%) and Alpha-pinene (S1:9.30%, S2:8.34%, S3: 9.17%) and other compounds in low percentage.

3

Heavy Metals Analysis and Quality Evaluation in Drinking Groundwater around an Abandoned Mine Area of Ouichane (Nador’s Province, Morocco)

Moubchir Tarik, Eloutassi Noureddine, Bendaoud Ahmed, Belkhiri Abdelkhalek, Maai Mohamed, Moubchir Majida, Zahir Ilham

Journal of Ecological Engineering

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2023

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Vol. 24, nr 5

118--127

EN

The eventual polluting of the Ouichane region’s groundwater by heavy metals around an abandoned iron mine was investigated. To reach this aim, the research began with a questionnaire survey to assess local people’s use of and appreciation for well and spring water, followed by measurement of spatial pollution load of heavy metals: Al, Ag, Fe, Cd, As, Cr, Co, Zn, Pb and Cu for water samples collected from twelve wells and three spring drinks of water using inductively coupled plasma-atomic emission spectrometry method (ICP). Determining the overall quality of spring and well water for human use was also performed by calculating the heavy metal pollution index (HPI). As result, the survey revealed that 44.90% of the households are not connected to the drinking water network, 97.3% of them use well water, which is highly appreciated, and 88.1% of the population consider its quality to be good to excellent. Meanwhile, the ICP analysis showed that all the water samples contain heavy metals. In fact, the maximum concentrations (expressed in 10-5 g/l) recorded per element were 9.7 for (Ag), 15 for (Al), 6.9 for (As), 4.5 for (Cd), 5.6 for (Co), 31 for (Cr), 14 for (Cu), 858 for (Fe), 7 for (Pb) and 2.9*10-5 g/l for (Zn). Moreover, most of the water samples recorded heavy metal values above World Health Organization (WHO) limits, for at least one metal among the ten tested, with high concentrations of iron observed in all samples. The HPI values for the three explored sources (S1, S2 and S3) and for 8 out of 12 wells (P3, P4, P5, P6, P7, P8, P9 and P10) exceed the critical pollution value and identify non-potable water with a high potential of contamination. Consequently, the results of this study raise the question about groundwater around this abandoned mining area, especially in the long term, the use of groundwater could increase because of the succession of years of drought on Moroccan territory and hence may constitute a significant health risk for most of the inhabitants.

4

Cactus and Holm Oak Acorn for Efficient Textile Wastewater Treatment by Coagulation-Flocculation Process Optimization Using Box-Benhken Design

Adachi Abderrazzak, Soujoud Radouane, El Ouadrhiri Faiçal, Tarik Moubchir, Hmamou Anouar, Eloutassi Noureddine, Lahkimi Amal

Journal of Ecological Engineering

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2023

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Vol. 24, nr 6

315--328

EN

In this study, the effectiveness of using natural bio-coagulants and bio-flocculants to treat textile wastewater through the coagulation-flocculation method was examined. These bio-based agents have several advantages over chemical agents, including biodegradability, natural abundance, low toxicity, and low cost. A bio-coagulant (holm oak acorn (HOA)) and a bio-flocculant (cactus juice) were used to investigate the capacity for turbidity removal and decolorization of textile wastewater. The UV spectrophotometer was used to characterize the discharges before and after treatment, and the chemical oxygen demand (COD) and biological oxygen demand (BOD5) levels were calculated. Box-Behnken design (BBD) coupled with response surface methodology (RSM) were utilized to optimize the process and reduce turbidity and decolorization in textile wastewater. The obtained results show that under the optimal conditions (0.5 g·L-1 of HOA, 15 mL·L-1 of cactus juice, and a pH of 7), decolorization and turbidity removal were achieved at 69% and 90%, respectively. This study demonstrates the potential of using bio-coagulants and bio-flocculants in the treatment of textile wastewater.

5

Physicochemical Characterization and Evaluation of the Organic Pollution of Domestic and Industrial Wastewater in the City of Fez

Bouzidane Zahra, Beniaich Ghada, Kaioua Samiha, Rezouki Sanae, Mansouri Dalal, El Mahjoub Aouane, Eloutassi Noureddine

Ecological Engineering & Environmental Technology

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2023

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Vol. 24, iss. 4

177-186

EN

Environmental concern implies a degree of basic dynamism aimed at safeguarding ecosystems against any type of pollution and better management of the environment. Wastewater is mainly composed of water and other materials that represent only a small part of wastewater, but can be present in sufficient quantities to endanger public health and the environment, so before discharging wastewater into the natural environment, it is necessary to define its parameters to have information on the composition and qualitative and quantitative characteristics of wastewater and their impacts on the receiving environment. The goal of the conducted work was to determine the degree of wastewater pollution in Fez based on the results of physicochemical analysis of samples collected from various stations in 2021. The temperature varies between 24.6°C and 29.9°C in wet periods and between 26.5°C and 38.3°C in dry periods, the pH is alkaline to neutral with high turbidity levels, especially in wet periods, for dissolved oxygen, the wastewater of the city of Fez is under saturated in oxygen, which accentuates anaerobic fermentation and the release of bad odors, and the average values of the BOD5 are higher than 100 mg O2/l considered. These wastewaters are rated as being of very poor quality. COD values are greater than 500 mg of O2/l, which is considered the limit value for direct rejections. Nitrate concentrations are highest in S1, with a value of 12.83 mg/l in the dry period. The recorded contents of orthophosphates and total phosphorus do not vary significantly. The obtained values are higher than the WHO recommended wastewater discharge standards. At the conclusion of this research, it was discovered that the wastewater in the city of Fez is of poor to very poor quality.

6

Evaluation of the Impact of Anthropic Activities on the Physico-Chemical and Microbiological Parameters of the Water of the Wadi Inaouene and Its Tributaries

Rezouki Sanae, El-Haji Sarra, Moubchir Tarik, Faouzi Jihan, Bendaoud Ahmed, Beniaich Ghada, Allali Aimad, Eloutassi Noureddine

Ecological Engineering & Environmental Technology

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2023

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Vol. 24, iss. 5

282--294

EN

Water is a basic human requirement that is required in many processes, particularly those performed in households. However, in most circumstances, this critical product does not fulfill commonly recognized safety criteria. Various anthropogenic activities cause various forms of pollution; as a result, their physicochemical and biological components can pose major hazards to the environment and human health. The objective of this research was to evaluate the microbiological parameters along the Inaouene wadi and its tributaries, as well as their correlation with the physicochemical characteristics. In spring 2020, the following physicochemical and microbiological studies were conducted: ICP-AES was used to assess metals. The most polluted stations are located upstream (S1, S3, S4, S5, and S6). The highest concentrations of sulfate, orthophosphate, BOD5 , and nitrogen compounds are found at these stations. Illegal solid and liquid discharges from the tributaries S1 and S5, as well as urban settlements built on the banks of the Inaouen River, are to blame for this pollution, which has a direct and significant impact on water quality throughout the wadi. Furthermore, heavy metals (Zn, Fe, Co, Cr, Ag, and Cu) are abundant upstream of Inaouen and pass downstream. The monitoring of the evolution of microbiological pollution of wastewater has revealed a significant contamination of these waters upstream of the Wadi by the 3 groups of germs indicative of a fecal contamination and the presence of Listeria monocytogenes and salmonella in the upstream stations and especially S1, S3 and S5. The PCA revealed that the indicator bacteria of fecal contamination present negative correlations with dissolved oxygen, thus the lack of dissolved oxygen leads to a proliferation of germs in the water.

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Contribution to the Treatment of Urban Wastewater in the City of Fez by Coagulation and Flocculation Using a Biodegradable Reagent

Elmansouri Ibtissame, Lahkimi Amal, Benaabou Mohamed, Chaouch Mehdi, Eloutassi Noureddine, Bekkari Hicham

Journal of Ecological Engineering

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2022

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Vol. 23, nr 2

77--85

EN

This study includes two parts; the first one, concerns evaluation of the physicochemical and microbiological quality of the urban wastewater of the city of Fez, through a space-time dynamics. In turn, the second part deals with the contribution to the treatment of these effluents, by a new biodegradable reagent in the process of physicochemical treatment (coagulation flocculation). For this purpose, the sampling of urban wastewater was carried out 4 months (September, December, February and June 2019), at a rate of two samplings per period on 4 specific sites along the river Fez, the degree of contamination of which differs from one site to another, according to space and time. The diagnostic of these stations showed a high level of pollution that is difficult to biodegrade. Indeed, this pollution can cause negative effects not only on the environment but also on human’s health. In order to take long-term action against the low quality of water and to replace certain inorganic coagulants, alternative processes for water treatment using cactus powder were set up. The characterization of results showed that the urban effluent has a high load in terms of electrical conductivity (EC), dissolved oxygen, chemical oxygen demand (COD), as well as concentrations of faecal coliforms, streptococci and staphylococci. In addition, the purifying power revealed that the removal rate of COD reaches maximum values of 38% and 58% forlimeand the mixture of lime with cactus powder, respectively.

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Chemical Composition and Antimicrobial Activity of Essential Oil of Wild and Cultivated Rosmarinus Officinalis from Two Moroccan Localities

Elyemni Majda, El Ouadrhiri Faiçal, Lahkimi Amal, Elkamli Taha, Bouia Abdelhak, Eloutassi Noureddine

Journal of Ecological Engineering

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2022

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Vol. 23, nr 3

214--222

EN

This research aimed to measure the impact of provenance on the yield, chemical profile and antimicrobial activity of Rosmarinus officinalis essential oil (EO) collected from cultivated and wild plants from two different regions in Morocco (Fez and Figuig). The chemical composition analysis was accomplished utilizing the GC-MS techniques. Sixteen compounds were identified in the EO of both samples, dominated by 1,8-cineole (32.18%), camphor (16.20%) and α-pinene (15.40%) in cultivated type. The α-pinene (51.19) presents the majority compound in the rosemary samples collected from the wild populations.The antimicrobial activity was investigated by using the broth dilution methods against yeast, four bacterial strains and two molds. Rosemary aerial part provided EO with the highest yield and comes from Figuig. The GC-MS analysis demonstrated the existence of two chemotypes of oils: α-pinene as well as 1,8-cineole/camphor/α-pinene. Both EOs showed good antimicrobial activity against all microbial strains. The essential oil produced by Fez plants was the most effective against the selected microorganisms having MIC values of 0.315–2.5 mg/L.

9

Study of the Operation of an Industrial Water Treatment Plant of the Northern Soft Drink Company Fez, Morocco

Elmansouri Ibtissame, Lahkimi Amal, Mansour Omar, Elouadrhiri Faical, Chaouch Mehdi, Eloutassi Noureddine, Elkhamar Fehmi, Bekkari Hicham

Ecological Engineering & Environmental Technology

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2022

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Vol. 23, iss. 6

227--232

EN

In order to verify the operation of the NSDC company’s industrial water treatment plant and to optimize the quality of the liquid discharges by coagulation and flocculation, we carried out the physico-chemical characterization of these liquid discharges and optimized the elimination of the organic matter. The results show that the abatement rate of the parameters SM, COD and BOD5 of the step are respectively 67%, 66% and 89% during November 79% 81% 89% during December and 69% 82% 89% during January. The treated wastewater showed that the WWTP is working properly, but in the coagulation flocculation treatment step instead of using a large amount of Dekfloc alone. We tried to optimize the dose of a plant powder as bio flocculant injected with Dekfloc as coagulant, which will increase the removal rate of organic matter. The tests of coagulation flocculation show that the application of the combination between Dekfloc and the powder of the cactus allows to increase the rate of abatement of COD to 54%, of BOD5 to 67%, of TSS to 80%, and therefore the reduction of microorganisms.

10

Impact of Irrigation Water on Heavy Metal Content in Irrigated Soils and Plants – Spatial and Vertical Distribution

Faouzi Jihan, Kaioua Samiha, Allali Aimad, Eloutassi Noureddine, Lahkimi Amal

Ecological Engineering & Environmental Technology

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2022

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Vol. 23, iss. 5

91--98

EN

It is essential to monitor the evolution of pollutants including heavy metals found in water intended for irrigation. Since they have a crucial impact on living beings. In this study, the contents of heavy metals were analyzed by ICP-AES on the three levels of irrigation water, soil and plants (olive tree and cardoon) and those in four different terrains. The Cd, Cu and Pd found in the water of the downstream Oued Fès and the treated wastewater are out of the norm. We found Pd in higher concentrations in both plants. For Cu it is more assimilated by the olive tree. Regarding the soil, the content of Cr is very high in the plot irrigated by water from Oued Fès located downstream and less high in the plot irrigated by treated wastewater. The cardoon of this last plot present an increased concentration in Cr more than the others. These variations depend on the type of plants and their different characteristics of absorption according also to the accumulation of metals in the soil adding also anthropic factors.