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PL
Fluorescencja znajduje obecnie ogromne zastosowanie w obrazowaniu komórek i badaniu dynamiki procesów komórkowych. Wynika to z dostępności różnorodnych fluoroforów oraz białek fluorescencyjnych, a także z wykorzystania w badaniach licznych technik fluorescencyjnych: FCS, FRET, FLIM, TIRF, STED oraz mikroskopii korelacyjnej, łączącej techniki fluorescencyjne i elektronowe.
EN
Fluorescence is widely applied in cell imaging and in studying cellular dynamics. Its popularity stems from availability of numerous fluorophores and fluorescent proteins as well as development of fluorescence techniques like FCS, FRET, FLIM, TIRF, STED, and correlative microscopy that combines the fluorescence and electron microscopy.
EN
This preliminary study examines the potential effects of diagenetic processes on the oxygen-isotope ratios of bone and tooth phosphate (δ18O) from skeletal material of individuals representing the Corded Ware Culture (2500–2400 BC) discovered in Malżyce (Southern Poland). Intra-individual variability of Ca/P, CI, C/P, collagen content (%) and oxygen isotopes was observed through analysis of enamel, dentin and postcranial bones. Using a variety of analytical techniques, it was found that, despite the lack of differences in soil acidity, not all the parts of a skeleton on a given site had been equally exposed to diagenetic post mortem changes. In a few cases, qualitative changes in the FTIR spectrum of analysed bones were observed. The data suggest that apart from quantitative analyses, i.e., the calculation of Ca/P, CI, C/P and collagen content, qualitative analyses such as examination of the absorbance line are recommended. The degree to which a sample is, contaminated on the basis of any additional, non-biogenic peaks, deemed to be contaminated should also be specified.
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