Wyniki wyszukiwania - BazTech

1

Fast and Sensitive RP-HPLC—Fluorescence Method for the Quantitative Analysis of Moxifloxacin in Rat Plasma and Its Application to a Preclinical Pharmacokinetic Study

Hurtado F. K., Kaiser M., Tasso L., Costa T.

Acta Chromatographica

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2016

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Vol. 28, no. 2

175--191

EN

A fast, simple, and sensitive reversed-phase high-performance liquid chromatography (RP-HPLC) method has been developed and fully validated for the determination of moxifloxacin (MXF) in rat plasma. MXF and gatifloxacin (internal standard, I.S.) were extracted from plasma by single-step protein precipitation with acidified acetonitrile. Chromatographic separation was accomplished in less than 8 min on an Atlantis ® T3 column with 0.4% aqueous triethylamine–methanol–acetonitrile (60:35:5, v/v/v) solution as mobile phase. Detection was achieved by fluorescence (λexcitation = 295 nm, λemission = 500 nm), and the calibration curves were found to be linear over the plasma concentration range of 10–2,500 ng mL−1 with a mean correlation coefficient (r) of 0.9946 (n = 6). The intra- and inter-assay imprecision (% CV) was less than 2.4 and 3.3%, respectively, and the accuracy was >90%. The mean extraction recoveries for MXF and I.S. from plasma were 77 and 82%, respectively. The method was also validated for specificity, sensitivity, and stability; all the results were within the acceptable range. The proposed method was then successfully applied to the quantitative analysis of MXF in rat plasma samples, being a valuable and high-throughput assay to support ongoing pharmacokinetic studies on this promising anti-infective agent.

2

Development and validation of a sensitive and selective LC—MS/MS method for the determination of an antimalarial drug candidate in rat plasma, and its application to a preclinical pharmacokinetic study

Pires C., Kaiser M., Grünspan L. D., Barreto F., Innocente A., Gnoatto S., Laureano J. V., Araujo B., Costa T., Tasso L.

Acta Chromatographica

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2016

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Vol. 28, no. 4

439--453

EN

An accurate and reliable LC—MS/MS assay was firstly developed and validated for quantitative determination of a new antimalarial prototype drug, 3β-hydroxyurs-12-en-28-oic acid (LAFIS 01), in rat plasma. Dexamethasone was employed as internal standard. Simple protein precipitation by acetonitrile for the sample preparation was used. Effective separation was achieved with Phenomenex Luna C18 (50 × 2 mm, 5 μm) column. The mobile phase consisted of (A) water and (B) acetonitrile, both containing 0.1% acetic acid, delivered by gradient elution. The column temperature was maintained at 40 °C. The LAFIS 01 was monitored by electrospray ionization interface, operating in the negative mode (ESI−) in multiple reactions monitoring (MRM), checking the transitions 455 > 455 for LAFIS 01 and 451 > 361 for the IS. Once LAFIS 01 demonstrated low fragmentation by collision-induced dissociation (CID) nonpresenting abundant high-intensity fragments to meet the desired concentration levels quantification, only pseudomolecular ion was monitored. The flow rate was 500 μL min−1. The lower limit of quantitation achieved was 10 ng mL−1 and linearity was observed from 10 to 500 ng mL−1. The relative standard deviation (RSD) values of the intra- and inter-assay precisions of the method were below 8.42 and 7.94%, respectively. The accuracy ranged from 92.05 to 102.94%. The extraction recovery of LAFIS 01 and IS was up to 90%. The method showed linearity, precision, accuracy, sensitivity, and stability required to quantify LAFIS 01 in preclinical pharmacokinetic study.

3

Channel Utilization in South Louisiana Using AIS Data, 2011-2012

Kaiser M.

TransNav : International Journal on Marine Navigation and Safety of Sea Transportation

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2014

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Vol. 8 no. 4

515--522

EN

South Louisiana is undergoing rapid land loss and the construction and utilization of navigation channels by the offshore oil and gas industry, the commercial fishing industry, the oil refining industry, and freight shippers is one cause. A network of natural and man-made navigation channels support commerce and industry throughout the region, but no quantitative information is available on the users of the channels and their contribution to land loss. The purpose of this note is to characterize utilization across eight channels in South Louisiana using data from the Automatic Identification System. Approximately 125,000 vessels used the channels over a two year period between 2011-2012. The Mississippi River was the most heavily utilized channel with an average of 345 vessels per week across the report zone, followed by Bayou Lafourche and Sabine Pass with about 195 vessels per week. The oil refining industry was the primary user of the Calcasieu and Sabine Pass channels while the freight industry was the primary user of the Mississippi River. The offshore oil and gas industry were the primary users of Bayou Lafourche, the Houma Navigation Canal, the Atchafalaya River and Freshwater Bayou.

4

Badania tunelowe prezepływu powietrza w otoczeniu wysokich budynków

Kaiser M.

Prace Instytutu Lotnictwa

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2008

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Nr 3-4 (194-195)

189-191

PL

W artykule przedstawiono wyniki badańi eksperymentalnych w tunelu aerodynamicznym, modeli wysokiej zabudowy aglomeracji miejskiej. Celem bylo oszacowanie zaburzeń wywołanych przepływem i interferencją opływu budynków dla oceny zagrożeń w wyniku wzmożonej turbulencji, mogacych mieć istotny wpływ na loty śmigłowców prowadzone w akcjach specjalnych w miastach i wykonywanych w bezpośredniej bliskości budynków.

EN

In a following article the results of experimental research of flow field part of around urbanistic aglomeration in wind tunnel has been shown. Main goal of this research was an estimation of disturbances caused by flow and interference with flow around the buildings to evaluate the threats caused by the increased turbulence, which influences on special mission helicopters flight in cities in close proximity of buildings and its cannot be neglected.

5

Extended Reynolds analigy for different flow conditions of the heated plate

Wierciński Z., Kaiser M., Żabski J.

Prace Instytutu Lotnictwa

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2007

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Nr 4(191)

83-91

EN

The investigation of the extended Reynolds analogy of the transitional boundary layer on the heated plate with different onflow condition was carried out. The coefficients of intermittency were determined on the basis of the tree-parametric cumulative distribution function of Weibull using the local shear stress Cf and Stanton number St distributions along the plate which are experimentally measured. For the unsteady flow with wakes the extended Reynolds analogy coefficient s is equal to 1.10 while for the flow without unsteadiness it is equal to 1.0. Finally, using the cumulative distribution function of Weibull the formula was proposed for the Prandtl number distribution in the region of laminar-turbulent transition.

PL

W pracy przedstawiono rezultaty badań rozszerzonej analogii Reynoldsa w warstwie przyściennej na grzanej płycie w różnych warunkach jej opływu. Na podstawie pomiarów lokalnego współczynnika oporu Cf oraz liczby Stantona wzdłuż płyty w strumieniach o różnym poziomie turbulencji wyznaczono rozkład współczynnika intermitencji jako trójparametrowej dystrybuanty rozkładu Weibulla. Dla niestacjonarnego przepływu ze śladami spływowymi wartość współczynnika rozszerzonej analogii Reynoldsa wynosi 1.10, zaś w pozostałych badanych przypadkach wartość tego współczynnika wynosi 1. Ponadto, zaproponowano zastosowanie rozkładu współczynnika intermitencji w postaci dystrybuanty trójparametrowego rozkładu Weibulla do wyznaczenia rozkładu liczby Prandtla w obszarze przejścia laminarno-turbulentnego w warstwie przyściennej dla przepływów o różnym poziomie niestacjonarności.

6

Analogia wymiany ciepła i pędu przy niestacjonarnym opływie płyty

Kaiser M.

Zeszyty Naukowe Instytutu Maszyn Przepływowych Polskiej Akademii Nauk w Gdańsku

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2006

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nr 543/1502

1-144

7

Extended Reynolds Analogy Coefficients of a Heated Flat Plate in Instationary Flow

Wierciński Z., Kaiser M.

Zeszyty Naukowe. Cieplne Maszyny Przepływowe - Turbomachinery / Politechnika Łódzka

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2005

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nr 128, vol. 2

581-588

EN

The investigation of the extended Reynolds analogy of the transitional boundary layer on the heated flat plate with different onflow condition was carried out. The coefficients of intermittency were determined on the basis of the tree-parametric cumulative distribution function of Weibull using the local shear stress Cf and Stanton number St distributions along the plate. For the unsteady flow with wakes the extended Reynolds number coefficient s is equal to 1.10 while for the flow without unsteadiness it is equal to 1.0. Finally, the formula was proposed for the Prandtl number distribution in the region of laminar-turbulent transition.

8

Transient laminar-turbulent transition of thermal boundary layer after the change of incidence angle

Wierciński Z., Kaiser M.

Transactions of the Institute of Fluid-Flow Machinery

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2003

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nr 114

237-252

EN

Convective heat transfer problems occur in many technical application. Unsteady transition in the heated boundary layer is one of them. Heat transfer investigation of a flat plate in a low subsonic wind tunnel for flow velocity Uo=15 and 20 m/s was performed. The changes in time of the Stanton number distribution along the plate were observed after the incidence angle had been changed from - 0.8 to 0°. While for the incidence angle i= -0.8° the flow in boundary layer was laminar, for angle -0.67#176 the transition was observed, and for the angle -0.23° the flow was turbulent. After the sudden change of angle the transient bahaviour of the heat transfer between these three states were observed, e.g. for the change from -0.8 to -0.6° from laminar to transitional, so after the time of about 30 min the transition was perfect. This time is needed to achieve a new internal thermal balance of the plate and transition in thermal boundary is established.

9

Flow field behind the wake generator

Kaiser M., Wierciński Z.

Transactions of the Institute of Fluid-Flow Machinery

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2003

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nr 112

3-20

EN

The investigation of the non-stationary flow behind the wake generator is presented. The mean velocity and rms velocity fluctuations are measured. The coordinates of points where the wakes from the windward side of the generator are intercepted by the wakes (rods) from the leeward of the generator are determined. The co-ordinates of these points depend on the diameter of the generator, the velocity of the oncoming flow and the frequency of rotation, i. e. the Strouhal number of the generator. The results of calculation are compared with the measurements and a good compatibility has been obtained. At the points of the wake cutting an increased fluctuations level is measured. The results of these investigations can be used in the study of the boundary layer transition induced by wakes with the influence of the wake cutting effect. This phenomenon is known in the turbomachinery as the so called clocking effect where they generate regions of enhanced turbulence level.

10

Struktura przepływu za generatorem śladów spływowych

Kaiser M.

Zeszyty Naukowe Instytutu Maszyn Przepływowych Polskiej Akademii Nauk w Gdańsku

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2002

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nr 530/1489

1-41

11

Zastosowanie foliowej sondy dwuwłóknowej do pomiarów turbulencji

Kaiser M., Wierciński Z.

Zeszyty Naukowe. Cieplne Maszyny Przepływowe - Turbomachinery / Politechnika Łódzka

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2000

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nr 117, vol. 2

73-79

PL

Termoanemometryczne sondy foliowe, przede wszystkim ze względu na ich trwałość, znajdują coraz częstsze zastosowanie. Jednakże ich właściwości dynamiczne nie są poznane dostatecznie dobrze. Badania przedstawione w niniejszej pracy wskazują na wysoki poziom szumów własnych tej sondy spowodowany śladem aerodynamicznym i czujnika. Badania przeprowadzono w strumieniu o niskim poziomie turbulencji i , porównano z pomiarami turbulencji wykonanymi zwykłą jednowłóknową sondą termoanemometryczną. Szum aerodynamiczny sondy foliowej jest ponad 25 razy większy od szumu sondy z grzanym drutem. Fakt ten należy uwzględnić przy wykorzystaniu tych sond do pomiarów turbulencji.

EN

Application of the split-fiber probes in the experiments steadily rises mostly due to , their durability. Their dynamics characteristics are rather not well recognized. Investigations presented in this paper point at the high level of aerodynamics noise of these probes, caused mainly by the How around they. Measurements were made in stream of the very low level of turbulence and compared with results of the hot-wire probe. The aerodynamic noise (velocity fluctuations caused by the probe) and its spectra were measured. The aerodynamic noise of the split-fiber probes is more than 25 times greater than that of the hot-wire probe. This fact is to be taken into consideration for measurements of turbulence by means of split-fiber probes.

12

Solution to reliability of machinery for brown coal surface mines trough introduction of adequate maintenance system

Helebrant F., Kaiser M.

Prace Naukowe Instytutu Górnictwa Politechniki Wrocławskiej. Konferencje

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1998

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Vol. 82, nr 21

171-180

EN

Solving the system of maintenance by means of the system theory enables us to examine behaviour of complicated system by disintegration into particular elements and to study their interrelations and relations to the surrounding which helps us solve the following fields of operating maintenance: strategy and conception, organization maintenance system, forms and methods of check-inspectional and revisional activities, forms and methods of repair control.

PL

Dział remontu jest jednym z podstawowych procesów obsługi każdej produkcji. Należy do procesów dwuznacznych. Po jednej stronie dochodzi do zużycia części, po drugiej - dochodzi do usuwania działań zużycia i wydłużania czasu eksploatacji i wyższej sprawności. Jest to jedna z dziedzin, które wpływają na obniżenie kosztów produkcji. Rozwiązanie funkcjonowania remontu według teorii systemowych pozwala analizować systemy złożone za pomocą rozkładu na poszczególne jednostki, analizować powiązania pomiędzy tymi jednostkami systemu oraz względem otoczenia. W wyniku można otrzymać następujące rozwiązania z zakresu remontu: - strategia i koncepcję, - systemy organizacji remontu, - formy i metody kierowania napraw,- systemy informacji i kierowania remontu, - podanie miejsca i zadania trybologii, trybotechniki oraz rozwoju technicznego, - ustanowienie ilości i składu działów remontu według profesji i tym samym rozwiązać problematykę funkcjonowania techniki wydobywczej z punktu widzenia odpowiedniej działalności działu remontu. Application of linguistic variables to determination of belt wear degree - method of scheduling conveyor belts for replacement. Zastosowanie zmiennych lingwistycznych do określenia stopnia zużycia taśmy - metoda szeregowania taśm przenośnikowych do wymiany.