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1
Content available The growth of Saos-2 cells on DLC layers doped with titanium
Travnickova M., Filova E., Jelinek M., Kocourek T., Bacakova L.
Engineering of Biomaterials
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2016
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Vol. 19, no. 138 spec. iss.
67
2
Content available Micro- and nanopatterned surfaces for guided adhesion, growth and phenotypic maturation of cells
Bacakova L., Filova E., Grausova L., Vandrovcova M., Parizek M., Novotna K., Svorcik V., Vacik J., Rypacek F., Kromka A., Heitz J., Shard A.
Engineering of Biomaterials
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2009
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Vol. 12, no. 89-91
18--21
EN
Micropatterned surfaces were created by UV light-irradiation of polytetrafluoroethylene through a metallic mask, by successive plasma polymerization of acrylic acid and 1,7-octadiene, or by creation of prominences and grooves by deposition of fullerenes C60 through a metallic mask. All these surface types were capable of inducing regionally-selective adhesion, proliferation and phenotypic maturation of vascular endothelial cells, vascular smooth muscle cells or human bone-derived MG 63 cells. Nanopatterned surfaces created by tethering GRGDSG oligopeptides through polyethylene oxide chains on a polymeric surface promoted spreading, formation of focal adhesion plaques and DNA synthesis in vascular smooth muscle cells. Surfaces nanopatterned with nanocrystalline diamond gave good support for the adhesion, growth and metabolic activity of osteoblast-like MG 63 cells.
3
Content available Endothelial cells on pet vascular prostheses impregnated with polyester-based copolymers and coated with cell-adhesive protein assemblies
Chlupac J., Filova E., Riedel T., Brynda E., Pamuła E., Lisa V., Bacakova L.
Engineering of Biomaterials
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2008
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Vol. 11, no. 81-84
108--111
EN
Arterial bypass surgery with synthetic vascular prostheses achieves poor patency rates compared to autogenous natural materials, and this is a challenge for tissue engineering research concerning small caliber vascular grafts. Modifications of the prosthetic surface followed by endothelial cell seeding may reduce thrombogenicity and intimal hyperplasia. Planar polyethylene terephthalate (PET) vascular prosthetic samples were impregnated with the copolymer poly(glycolide-L-lactide) (PGL) or with the terpolymer poly(glycolide-L-lactide-(e)caprolactone) (PGLCap) in order to lower the permeability of the knitted fabrics and ensure a less adhesive background. Subsequent modification with adhesive protein assemblies composed of collagen type I (Co) in conjunction with laminin (LM), fibronectin (FN) or fibrin (Fb) gel was performed to enhance cell adhesion. Bovine pulmonary artery endothelial cells (EC) of the CPAE line were seeded on to the coatings and subjected to static tissue culture conditions for 7 days. Impregnation of the PET prostheses decreased the initial adhesion and proliferation of the EC. After coating with the protein assemblies, the impregnated PET provided better substrates for cell culture than the protein-coated PET, on which the EC population started decreasing after 4 days of culture. The cells proliferated better on the CoFN, CoFb and CoFbFN coatings than on the Co and CoLM coatings. Impregnation type and adhesive matrix protein deposition may play an important role in successful endothelialization, healing and clinical performance of vascular grafts.
4
Content available Human endothelium on vascular prostheses modified by extracellular matrix proteins in a flow experiment
Chlupac J., Filova E., Riedel T., Brynda E., Remy-Zolghadri M., Bareille R., Fernandez P., Daculsi R., Bordenave L., Bacakova L.
Inżynieria Biomateriałów
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2006
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R. 9, nr 58-60
10-13
EN
Artificial vascular prostheses are used for bypass surgery. Thrombogenicity often cause graft occlusion. Targeted surface alterations including cell seeding may improve the haemocompatibility. Knitted commercial tubular PET (polyethylene terephtalate) vascular prostheses with collagen impregnation were modified by adsorption of laminin (LM) or coating with fibrin network (FB) on the luminal surface. Human endothelial cells were harvested, cultured and seeded at the density of 150x10\3 cells/cm square on all grafts. The cell lining was continuously visualized and quantified. The retention was 21%, 37% and only 2% of the seeding density on the unmodified (UM), LM- and FB-coated grafts, respectively. These seeded prostheses were exposed to a laminar shear stress (SS) 15 dynes/cm square for 40 minutes (UM, LM, FB) and 120 min (UM, LM) in a chamber simulating blood circulation. The SS was excluded in static (ST) control grafts. After 40 min-SS the cell numbers were78%, 27% and 72% for the UM, LM and FB prosthesis compared to the ST. The cell densities were 61% and 57% on the UM and LM after 120 min-SS. To conclude, the endothelium formed a confluent layer whereas laminin immobilization improved endothelial adhesion but not the flow resistance. Reverse effect was observed on fibrin coating.
5
Content available Adhesion and growth of vascular smooth muscle cells on protein assemblies for biomaterial coating
Filova E., Brynda E., Houska M., Riedel T., Bacakova L.
Inżynieria Biomateriałów
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2005
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R. 8, nr 47-53
9--12
EN
We investigated adhesion and growth of vascular smooth muscle cells (VSMC) on proteins assemblies prepared by coating of thin fibrin net with ultrathin layer of collagen I, fibronectin or laminin. The assemblies were deposited on polystyrene (PS) non-modified with plasma discharge, i.e. a treatment commonly used for creation of surfaces suitable for cell cultivation. All samples with the fibrin assemblies improved initial attachment and spreading of VSMC as well as their subsequent growth in comparison with the pristine PS. The adhesion area of VSMC increased on fibrin coated with collagen I and fibronectin, while on laminin it remained similar as that on the pure fibrin. Thin 3D fibrin net markedly changed the morphology of VSMC from polygonal to star-like shape with protrusions. Cells all tested samples formed vinculin-containing focal adhesion plaques and α-actin cytoskeleton, an important marker of VSMC differentiation. The protein assemblies can be used for improvement of cell adhesion and growth on various artificial materials used in tissue engineering, e.g. for construction of bioartificial vascular prostheses.
6
Content available The influence of chemical structure of aliphatic polyesters on adhesion and growth of osteoblast-like MG63 cells
Pamuła E., Bacakova L., Buczyńska J., Filova E., Noskova L., Dobrzyński P., Bero M.
Inżynieria Biomateriałów
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2004
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R. 7, nr 37
14-17
EN
Degradable copolymers of glycolide and L-lactide (PGLA), glycolide and epsylon-caprolactone (PGCap) and terpolymer of glycolide, L-lactide and epyslon-caprolactone (PGLCap) were synthesized by ring opening polymerization using zirconium acetylacetonate (Zr(acac)4) as a biocompatible initiator. The structure and physicochemical surface properties of the materials were studied by NMR spectroscopy, gel permeation chromatography, X-ray photoelectron spectroscopy and senssile drop. The interaction of polymeric films produced by slip-casting with osteoblast-like MG63 cells were tested in vitro. The number of adhering cells, their shape and the size of cell-material contact area were evaluated from day 1 to 7 after seeding. It was found that the cell behaviour on PGLA and PGLCap was very similar as on control tissue culture polystyrene (TCPS). On PGCap, however, the number of initially adhering cells was significantly lower (by 84% than on TCPS) and cell spreading area smaller (by 50% than on TCPS). On day 7 after seeding, these cells reached the lowest population density (by 30% smaller than TCPS). We hypothesize that the lower cell adhesion and growth of MG63 cells on PGCap was caused by the highest hydrophobicity of this material among all studies samples.
7
Content available Adhesion and proliferation of vascular smooth muscle cells on polylactide-polyethylene oxide copolymers with different content and length of polyethylene oxide chains
Filova E., Bacakova L., Lisa V., Kubies D., Machova L., Lapcikova M., Rypacek F.
Inżynieria Biomateriałów
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2004
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R. 7, nr 38-42
19-21
EN
We evaluated antiadhesive effects of polymer surfaces prepared from PDLLA-PEO copolymers using PEO with a different molecular weight and different PEO content in comparison with the native poly(L-lactide) (PLLA) surface. All PDLLA-PEO copolymers significantly decreased number of initially adhered cells (by 23-55% in comparison with pure PLLA) as well as spreading area 24 hours after seeding (by 39-79%). Cell proliferation, estimated by cell number on the 6 day after seeding and bromodeoxyuridine (BrdU) labeling index, was significantly lower on PEO-containing copolymers (by 58-96% and 21-35% respectively) compared to pure PLLA surface. Immunofluorescence staining of vinculin showed that the ability of VSMC to form local adhesion plaques was markedly reduced on surfaces with the highest content of PEO (33 and 44%). Thus, these copolymers are promising for creation of surfaces preventing uncontrolled adsorption of proteins and adhesion of cells. Consecutively, binding of defined ligands for cell adhesion receptors would enable to control cell behaviour on these materials, which could be used for construction of vascular prostheses.
8
Content available Vascular smooth muscle cells in cultures on lactide based polymers for potential construction of artificial vessel wall
Filova E., Bacakova L., Lisa V., Machova L., Lapcikova M., Kubies D., Proks V., Rypacek F.
Inżynieria Biomateriałów
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2003
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R. 6, nr 30-33
9-11
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