Wyniki wyszukiwania - BazTech

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Impact of human serum on HAp-glucan composite

Borkowski L., Lübek T., Jojczuk M., Nogalski A., Belcarz A., Pałka K., Hajnos M., Ginalska G.

Engineering of Biomaterials

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2018

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Vol. 21, no. 148

10

2

Effect of a low pH on HAp/glucan composite

Borkowski L., Kiernicka M., Belcarz A., Pałka K., Hajnos M., Ginalska G.

Engineering of Biomaterials

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2016

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Vol. 19, no. 138 spec. iss.

22

3

Oznaczenie zawartości jonów Zn2+ i Ca2+ w roślinach

Szatanik-Kloc A., Boguta P., Sokołowska Z., Hajnos M., Alekseeva T., Alekseev A.

LAB Laboratoria, Aparatura, Badania

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2011

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R. 16, nr 1

16-18

PL

Do oznaczenia zawartości cynku i wapnia w ekstraktach z korzeni i części naziemnych żyta ozimego odmiany Rostockie, wykorzystano Absorpcyjny Atomowy Spektrometr AAS 3300. Badane rośliny pochodziły z uprawy hydroponicznej. W fazie rozwoju strzelanie w źdźbło, do pożywki dodatkowo zaaplikowano fitotoksyczne ilości jonów Zn2+ (jako ZnC12) w stężeniach 20, 200, 400 mg • dm3 pożywki. Zaobserwowano istotny wzrost zawartości cynku w korzeniach i w częściach nadziemnych badanych roślin. Jednocześnie fitotoksyczne stężenia cynku w pożywce wpłynęły istotnie na obniżenie zawartości jonów wapnia w korzeniach i w częściach nadziemnych żyta.

4

Exploration of the possibility of combined HS-GC-MS and 13C NMR spectroscopic investigation of the composition of the essential oils of selected sage (Salvia) species

Sajewicz M., Matlengiewicz M., Rzepa J., Wojtal Ł., Hajnos M., Waksmundzka-Hajnos M., Kowalska T.

Acta Chromatographica

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2011

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Vol. 23, no. 1

181--190

EN

The objective of this study was to explore the possibility of combining headspace gas chromatography with mass spectrometric detection (HS-GC-MS) and 13C NMR spectroscopy to enhance the detectability of components of the essential oils of medicinal plants of the Salvia genus. Preliminary investigations were performed with two sage species, Salvia lavandulifolia and Salvia triloba, known for particularly abundant yields of their respective essential oils. By use of HS-GC-MS, characteristic fingerprints of the volatile fractions were obtained for plant species from two different vegetation seasons (2007 and 2008). Partial identification of the components of these volatile fractions was performed, and comparison of the chromatographic fingerprints confirmed seasonal fingerprint similarity within the same species. Preliminary 13C NMR measurements resulted in well shaped spectra with an abundance of regularly distributed signals. This suggested the possibility of using 13C NMR spectroscopy in phytochemical research, in parallel with HS-GC-MS, to enhance the detectability of volatile components of plant species belonging to this genus.

5

Multidimensional TLC procedure for separation of complex natural mixtures spanning a wide polarity range; Application for fingerprint construction and for investigation of systematic relationships within the Peucedanum genus

Cieśla Ł., Skalicka-Woźniak K., Hajnos M., Hawrył M., Waksmundzka-Hajnos M.

Acta Chromatographica

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2009

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Vol. 21, no. 4

641--657

EN

Identification of closely related plant species is not a trivial task, and is often difficult on the basis of morphological or anatomical features. To differentiate among analyzed species, a chromatographic fingerprint is usually constructed. This is not easy for very complex samples, however, especially those containing substances spanning a wide polarity range. In such circumstances more than one fingerprint is constructed, e.g. one for polar and the other for apolar constituents. In this paper a new method has been used for resolution of a mixture of 17 coumarins and flavonoids and, subsequently, construction of a fingerprint of closely related species belonging to the Peucedanum genus. This is the first time multidimensional planar chromatography has been applied for such purpose. Distinctive fingerprints are produced for every analyzed plant species, without the need to construct two fingerprints and without the application of more sophisticated multidimensional methods (e.g. hyphenation of HPLC and TLC, HPLC-MS, etc).

6

GC-MS study of the performance of different techniques for isolating the volatile fraction from sage ( Salvia L.) species, and comparison of seasonal differences in the composition of this fraction

Sajewicz M., Rzepa J., Hajnos M., Wojtal Ł., Staszek D, Kowalska T., Waksmundzka-Hajnos M.

Acta Chromatographica

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2009

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Vol. 21, no. 3

453--471

EN

Application of chemotaxonomy to the plant kingdom can be regarded as a future challenge to the traditional binomial classification system. Construction of a classification system based on the chemical composition of plants has only recently become possible with the development of sophisticated chromatographic and hyphenated techniques. Successful chemotaxonomy can, however, be achieved only if sufficient experimental evidence is collected confirming the reproducibility of the chemical composition of plant species (irrespective of the specimen, its growing season, location of the collection site, etc.). The objective of this study was to compare, by use of gas chromatography with mass spectrometric detection (GC-MS) the efficiency of isolation of volatile compounds from five different Salvia L. (sage) species ( S. lavandulifolia, S. staminea, S. hians, S. triloba , and S. nemorosa ) by use of four different techniques [head-space extraction at 70 and 80°C, vapour distillation in a Deryng apparatus, and accelerated solvent extraction (ASE)]. We also compared the composition of the volatile fractions of these species collected during two different seasons (2007 and 2008). It was established that the composition of the volatile fractions is very dependent on the species considered and, in a much less pronounced way, on the growing season. This statement is valid irrespective of the technique used to isolate the volatile compounds from the plant matrix. The seasonal reproducibility of the compounds regarded as chemotaxonomic markers and chemotaxonomic advice compounds (and the repeated absence of such compounds from a species) makes the volatile fraction suitable for chemotaxonomic evaluation of sage. Finally, it was shown that head-space extraction of the volatile fraction at 70°C was the best extraction technique for the purpose of this study.

7

Comparative analysis of the chromatographic fingerprints of twenty different sage ( Salvia L.) species

Daszykowski M., Sajewicz M., Rzepa J., Hajnos M., Staszek D, Wojtal Ł., Kowalska T., Waksmundzka-Hajnos M., Walczak B.

Acta Chromatographica

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2009

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Vol. 21, no. 4

513--530

EN

Comparative analysis of twenty different sage ( Salvia L.) species grown in Poland has been performed on the basis of two types of chromatographic fingerprints. For efficient preprocessing and comparison of these fingerprints, chemometric methods were used. The main emphasis was on preprocessing of herbal fingerprints and selecting a suitable preprocessing strategy for exploring differences among them. After successful preprocessing of the fingerprints, principal component analysis was used to reveal chemical differences among the samples. An outcome of the comparative analysis was to pinpoint specific regions of the fingerprints indicative of differences among the samples. In fingerprints of the volatile fraction from the sage ( Salvia L.) species, obtained from head-space gas chromatography coupled with mass spectrometry, important regions were identified and associated with the presence of camphene, limonene, and eucalyptol in these samples.