Wyniki wyszukiwania - BazTech

1

The biocompatibility of membranes for immunoisolation

Granicka L., Kawiak J., Weryński A.

Biocybernetics and Biomedical Engineering

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2008

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Vol. 28, no. 2

59-68

EN

The assessment of biocompatibility of systems consisting of a host, permiselective membrane and a biological material, encapsulated within may be standardized for biological material immunoisolation purposes. The procedures for evaluation of the membrane non-toxicity against the biological material, the membrane physical and chemical stability post implantation and the biomaterial ability to perform with the host are presented.

2

The antibiotic induction of apoptotic - like changes in bacteria E. coli - GFP encapsulated in hollow fibers

Kawiak J., Granicka L., Żołnierowicz J., Weryński A.

Biocybernetics and Biomedical Engineering

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2008

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Vol. 28, no. 2

95-100

EN

Encapsulation of bacteria in a semipermeable polymer membrane gives a lot of technological possibilities. Our aim was to evaluate the performance of bacteria encapsulated in hollow fibers when treated with chosen antibiotic. The antibiotic application may cause release of biologically active substances for which production the bacteria may be genetically modified. The encapsulated in HF bacteria Escherichia coli transfected with pQE-GFP (green fluorescent protein) plasmid were incubated with addition of gentamycin or tetracycline. The encapsulated in hollow fibers E. coli culture with addition of tetracycline proves the tetracycline impact on the bacteria viability increasing the necrotic bacteria share. Polypropylene modified membranes allow to avoid permeation of the bacteria through the membrane wall. E. coli encapsulated in HF may be used in future, in systems releasing the therapeutic factor.

3

Repair of articular cartilage full thickness defects with cultured chondrocytes placed on polysulphonic membrane - experimental studies in rabbits

Płończak M., Czubak J., Hoser G., Chwojnowski A., Kawiak J., Dudziński K., Czumińska K.

Biocybernetics and Biomedical Engineering

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2008

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Vol. 28, no. 2

87-93

EN

Autologous osteochondral transplantation is one of the methods. that can be used to create hyaline or hyaline - like repair in the defect area. The purpose of the present study was to repair the full - thickness articular cartilage defects in nine rabbits' knee joints with autologous cultured chondrocytes. An articular cartilage defect was created on the patellar groove of the femur. The defect was filled with the chondrocytes cultured in vitro and placed into the knee on a polysulphonic membrane. 8 weeks after the operation the reparative tissue was analyzed macroscopically and histologically. The surfaces of the reparative tissue were smooth, and the defects were filled with mature hyaline cartilage in five cases. In two cases the reparative hyaline cartilage was immature and there was worse integration of the grafted tissue into the adjacent normal cartilage. The surface of the grafted area was irregular, the reparative tissue was desintegrated and incompletely differentiated. The results suggest that transplantation of the autologous chondrocytes cultured in vitro and placed into the knee on the polysulphonic membrane is effective in repairing of the articular cartilage defect.

4

3D matrigel culture improves differentiated functions of HepG2 cells in vitro

Kinasiewicz J., Kawiak J., Weryński A.

Biocybernetics and Biomedical Engineering

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2006

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Vol. 26, no. 4

47-54

EN

In our study, the influence of culture conditions (monolayer and 3D Matrigel) of hepatocyte cell line (buman hepatoma cell line, HepG2) on their metabolic activity was assessed. Albumin synthesis by HepG2 cells was significantly higher in Matrigel culture as compared to monolayer culture of the cells. 3D culture improved urea production by HepG2 cells. The detoxification experiments revealed that HepG2 cells do not metabolize lidocaine neither in monolayer nor in 3D culture. 3D culture significantly improved cell polarity analyzed by F-actin staining and carboxyfluorescein excretion. In conclusion, the 3D Matrigel culture improves highly differentiated hepatic functions of HepG2 cells.

5

BIAL - Registration and Monitoring System for Patients with chronic lymphocitic leukemia

Ładyżyński P., Migalska-Musiał K., Foltyński P., Kawiak J., Hus I., Sieklucka M., Roliński J., Dmoszyńska A., Wójcicki J.M.

Biocybernetics and Biomedical Engineering

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2005

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Vol. 25, no. 3

57-63

EN

B-cell chronic lymphocitic leukemia (B-CLL) is the most common type of leukemia in adults. The aim of the work was to design and implement a computer registration and monitoring system for patients with B-CLL. The developed BIAL system comprises modules allowing for registration of the data related to patient's identification, patient's general state (e.g. objective examination, phenotype, etc.), laboratory examinations (biochemical, immunological and cytogenetical), applied treatment (chemo- and/or immunotherapy) and evaluation of immunotherapy on an animal model. Selected biochemical and immunological parameters can be presented on charts, to facilitate evaluation of patient's state, the course and outcome of the treatment. The BIAL system was preliminary verified basing on the data registration of 8 B-CLL patients treated with chemo- or immunotherapy in the Clinic of Hematooncology and Bone Marrow Transplantation MU in Lublin. It was found to be comprehensive yet easy to learn and use. The system should facilitate the current treatment of the B-CLL patients and should make a foundation for the future multi-patients data analysis leading to elaboration of the criteria for selection of the optimal treatment regimen for patients with B-CLL.

6

Polypropylene surface modified hollow fibers for immunoisolation of cells

Granicka L. H., Chwojnowski A., Kawiak J.

Biocybernetics and Biomedical Engineering

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2004

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Vol. 24, no. 2

59-67

EN

Membranes preventing the tissue overgrowth as well as toxic influence of the environment on cells encapsulated within them were elaborated. The permiselective polypropylene hollow fibers were modified using different methods of silanization, and the effect of silanization on the membrane transport properties as well as on the tissue overgrowth was assessed, in the light of their perspective application in encapsulation of the cells. The membrane's pressure silanization has been found to result in obtaining membranes allowing to decrease and/ or delimit the diffusive transport through the membrane of large solutes without a simultaneous transport change for small solutes. The applied different types of membrane's silanization allowed to avoid a massive tissue overgrowth during the implantation. The membranes have been found to comply with the requirements for membranes applied in immunoisolation.

7

Badanie wpływu silanizacji na własności transportowe membran do immunoizolacji

Granicka L., Kaczmarek-Baranek Ż., Chwojnowski A., Kawiak J.

Zeszyty Naukowe. Inżynieria Środowiska / Politechnika Śląska

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2002

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z. 47

37-44

PL

Modyfikowano powierzchnię polipropylenowych, półprzepuszczalnych membran kapilarnych przy zastosowaniu różnych sposobów silanizacji, badając wpływ pokrywania powierzchni membran na ich własności transportowe z punktu widzenia przyszłych zastosowań in vivo. Badano też obrastanie tkanką łączną membran modyfikowanych różnymi sposobami we wszczepach in vivo. Stwierdzono, że badane membrany spełniają wymogi membran do immunoizolacji komórek.

EN

The permiselective polypropylene hollow fiber's surface was modified using different ways of silanization and the influence of silanization on membrane transport properties was assessed. The tissue overgrowth of different modified membranes was evaluated in vivo. It was found that the membranes comply with the requirements for membranes applied for immunoisolation.