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1 2013

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Development and validation of a reversed-phase liquid chromatography method for the quantification of meloxicam in liposomes

Montejo C., Civera C., Barcia E., Negro S., Fernández-Carballido A.

Acta Chromatographica

2013

Vol. 25, no. 4

639--653

In the present study, we have developed and validated an analytical method for the determination of meloxicam in liposomes using high-performance liquid chromatography-ultraviolet (HPLC-UV). Chromatographic separation was carried out on an Ascentis RP amide C16 column selecting a mobile phase composed of acetonitrile-0.3% formic acid solution (40:60, v/v) adjusted at pH 2.8. The mobile phase flow rate selected was 0.5 mL min -1 and UV detection at 355 nm. Piroxicam was chosen as internal standard. All the analyses were performed at temperatures of 40.0 ± 0.5°C. The calibration curve was linear over the range 18–420 ng mL -1. Relative standard deviation (RSD) for precision was <1.03%. Accuracy ranged between 98.53% and 101.41% with RSD lower than 1.5%. LOD and LOQ were 5 ng mL -1 and 15 ng mL -1, respectively. The method was simple, rapid, and easy to apply, making it very suitable for routine analysis of meloxicam in liposomes. The method could also be used with reliability for the determination of meloxicam in other pharmaceutical dosage forms.