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Testing of Bacterial and Fungal Isolates from Rumen Fluid Used in Inoculants in the Fermentation of Feed from Agro-Industrial Waste

Mustabi Jamila, Zulkharnaim, Kuswinanti Tutik, Sirajuddin Sitti Nurani, Al-Tawaha Abdel Razzaq

Journal of Ecological Engineering

2022

Vol. 23, nr 3

92--99

The addition of the inoculant can accelerate the fermentation process and improve the quality of the feed material in fermentation. This study aimed to isolate, test and select the rumen microorganisms that have high ability to degrade the fiber and improve the quality of materials used in inoculants to ferment agricultural wastes as alternative feed. Microbial isolate was extracted from cattle rumen fluid. The isolates type of fungus were grown on Potato Dextrose Agar (PDA) while the bacteria was grown on Nutrient Agar (NA) medium. Microbes of fungi were tested and selected based on their ability to produce ligninase, cellulase and hemicellulase enzymes. Bacteria were grown on MRS broth media as a confirmation for LAB colonies, then tested for gram staining, catalase test, growth on different NaCl levels (4 and 6.5%) and growth at various pH (3, 4, and 5). The results showed that five fungal isolates had a growth diameter of 0.1–0.37 mm on lignin, cellulose and hemicellulose media after seven days. Eleven bacterial isolates were indicated as lactic acid bacteria; almost all isolates could grow on NaCl 6.5%, but only four bacterial isolates could grow on NaCl 4% and five bacterial isolates could grow at pH 3. In conclusion, three isolates of fungi have a remarkable ability to degrade lignin. Five isolates of lactic acid bacteria have a comprehensive ability as a preservative in fermentation, so eight microbial isolates from rumen fluid can be used as inoculants in fermenting the feed material from agro-industrial waste.

Growth, Yield and Biochemical Responses in Barley to DAP and Chitosan Application Under Water Stress

Al-Tawaha Abdel Rahman M., Jahan Nusrat, Odat Nidal, Al-Ramamneh Ezz Al-Dein, Al-Tawaha Abdel Razzaq, Abu-Zaitoon Yousef M., Fandi Khalid, Alhawatema Mohammad, Amanullah, Rauf Abdur, Wedyan Mohammad, Shariati Mohammad Ali, Qaisi Ali M., Imran, Tawaha Khaled, Turk Munir, Khanum Samia

Journal of Ecological Engineering

2020

Vol. 21, nr 6

86--93

The present investigation was aimed at enhancing the quality and productivity of barley by the fertilizer and chitosan application. The field experiments were conducted in the northern region of Jordan, under the rain fed conditions in the main growing seasons of 2014/2015 and 2015/2016. The experiment was conducted in a well-designed split-plot having three replications and two fertilizer levels (0 and 100 kg•ha-1 DAP (Diammonium phosphate 46% P2O5). Chitosan, in three different concentrations (0, 5, and 10 g•L-1), was randomly applied to all fertilized plots as subplot treatments. The results revealed the highest seed dry weight (5.8 g per plant) in the plants treated with 100 kg•ha-1 of DAP, while the lowest (5.2 g per plant) was recorded in the control which exhibited an increase of about 10%. However, other parameters, namely the number of grains, number of spikes, and number of grains were also found to be influenced by the chitosan treatment. Significant variation (P < 0.01) were also high between the lines in the presence and absence of chitosan application. The highest number of grain yield, number of spikes, and grains/spike were found by the foliar treatment of 10 g•L-1 chitosan to barley plants at the tillering stage. Similarly, the grain quality, particularly with respect to protein and starch, was found to be enhanced significantly over control. The highest protein (12.6%) and starch (62.3%) were obtained with 100 kg•ha-1 DAP fertilizer level mixed with 10 g•L-1 chitosan. Hence, based on results, it can be concluded that the fertilizer level 100 kg•ha-1 DAP combined with 10 g•L-1 chitosan is economically best and recommendable for improving the quality and productivity of barley in the northern region of Jordan.