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PL
W artykule przedstawiono opis elektronicznych i programistycznych rozwiązań sprzętowego, mikrofalowego generatora losowych ciągów binarnych o przepływności wyjściowej 1 Gbit/s. Szczególną uwagę poświęcono problemom programowej obsługi procesów przetwarzania ciągów o tak dużej przepływności. Opisano autorską metodę pomiarów i weryfikacji entropii generowanych ciągów oraz przedstawiono warunkowy, kryptograficzny dowód ich bezpieczeństwa.
EN
The article presents a description of electronic and programming solutions for a hardware, microwave random binary sequences generator with an output bit rate of 1 Gbps. Particular attention was paid to the problems of programmatic handling of string processing processes with such a high throughput. Have been described the method of measuring and verifying the entropy of the generated strings and presents a conditional, cryptographic proof of their security.
PL
W artykule przedstawiono analizę możliwości sprzętowej generacji doskonale losowych ciągów binarnych z przepływnością 1 Gbit/s, wykorzystującą układy i sygnały mikrofalowe. W analizie użyto powszechnie znanych modeli generacji ciągów losowych, jednak z wykorzystaniem niestosowanych dotąd w tym celu technologii, w tym zastosowania do kreacji ciągów układów programowalnych FPGA. Analizy udokumentowano wynikami pomiarów rzeczywistych układów i sygnałów w funkcji czasu i częstotliwości za pomocą oscyloskopu i analizatora widma w paśmie 2 GHz.
EN
The article presents an analysis of the hardware capabilities of generating truly random binary strings with a bit rate of 1 Gbps, using microwave circuits and signals. The analysis uses commonly known models of random sequence generation, but with the use of technologies that have not been used for this purpose so far, including the use of FPGA programmable circuits for the creation of sequences. The analyzes were documented with the results of measurements of real systems and signals as a function of time and frequency using an oscilloscope and a spectrum analyzer in the 2 GHz band.
EN
The use of bone cement in procedures such as vertebroplasty and kyphoplasty can reduce pain and mechanically support the spine. This study aimed to evaluate whether air entrapped within bone cement affected its distribution in a vertebral body model. The study included 3D printed anatomical models of vertebrae together with their internal trabecular structure. Aeration was achieved by mixing the bone cement using three different altered procedures, whilst the control sample was prepared according to the manufacturer’s instructions. The further two samples were prepared by reducing or increasing the number of cycles required to mix the bone cement. A test rig was used to administer the prepared bone cement and introduce it into the vertebral model. Each time the injection was stopped when either the cement started to flow out of the vertebral model or when the entire cement volume was administered. The computer tomography (CT) scanning was performed to evaluate aerification and its influence on the bone cement distribution in each of the patient-specific models. The large number of small pores visible within the cement, especially in the cannula vicinity, indicated that the cement should not be treated as a homogenous liquid. These results suggest that a high level of aerification can influence the further cement distribution.
4
Content available remote Metoda i układ powielania ciągów losowych
PL
Przedmiotem pracy są metoda i układ powielania ciągów losowych. Powielanie polega na pseudolosowym próbkowaniu odpowiednio licznej próby ciągu prawdziwie losowego ze źródła w postaci generatora sprzętowego, a następnie na wymianie wykorzystanej próby na następną i skasowaniu próby zużytej. W wyniku powielania otrzymuje się ciągi wynikowe o zadowalających właściwościach probabilistycznych i parametrach statystycznych. Metoda będąca przedmiotem pracy pozwala na powielanie ciągów z generatorów sprzętowych do krotności sięgających 1000.
EN
The subject of the work is the method and system for duplicating random sequences. Reproduction consists in pseudo-random sampling of a sufficiently large sample of a truly random sequence from a source in the form of a hardware generator, and then on the exchange of the used sample for the next one and deletion of the used sample. As a result of duplication, result sequences with satisfactory probabilistic properties and statistical parameters are obtained. The method allows duplication of sequences from hardware generators up to 1000 times.
EN
The study involved the use of a bacterial strain isolated from environmental samples which produce the biopolymer in the form of pellets in the submerged culture. This material (bacterial exopolysaccharide) is produced by bacteria of the Komogateibacter xylinus which are prevalent in the environment. The aim of this study was to characterize bacterial exopolysaccharides and commercial dextran-based “microcarriers” in terms of their roughness and cell culture effects, including the morphology and viability of the human hybridoma vascular endothelial cell line EA.hy926. The pellets were characterized using scanning electron microscopy (SEM) and atomic for¬ce microscopy (AFM). The resulting structures were used for cell culture of adherent cells (anchorage¬-dependent cells). At the same time, the cultures with commercial, dextran-based “microcarriers” were carried out for comparative purposes. After com¬pletion of the cell culture (24 hours of culture), the cellulose and commercial “carriers” were analyzed using SEM and AFM. Finally, the obtained cell dens¬ities (fluorescence labelling) and their morphological characteristics (SEM) were compared. The obtained results strongly support the applicability of bacterial exopolysaccharide (EPS) in tissue engineering to build innovative 3D scaffolds for cell culture, the more so that it is technologically possible to produce EPS as spatially complex structure
EN
Nowadays nanostructures are more and more often designed as carriers for drug delivery, especially to improve the drug pharmacokinetics and pharmaco-dynamics. Numerous kinds of nanostructures are considered a good prospect for medical applications thanks to their small size, acceptable biocompatibility and toxicity. Due to the fact that nanotechnology is a new field of science, every nano-scale product must be thoroughly examined regarding its toxicity to the human body. This study provides new insights into effects of exposing endothelial cells to the selected nanostructures. Dendrimers of the fourth generation (PAMAMs), multi-walled carbon nanotubes (MWCNTs) and silver nanoparticles (SNPs) were used to evaluate nanostructures influence on endothelial cells in vitro. The nanostructures were evaluated via transmission electron microscopy and dynamic light scattering technique. The cells previously exposed to the nanostructures were observed and analyzed via the atomic force microscopy and scanning electron microscopy to obtain a quantitative evaluation of the cells morphology. The presence of multi-walled carbon nanotubes and silver nanoparticles on the cells surface was confirmed by the scanning electron microscopy. Our results confirm that the surface association and/or uptake of nanostructures by the cells resulting from physicochemical and biological processes, affect the cells morphology. Morphological changes can be induced by the membrane proteins interaction with nanomaterials, which trigger a sequence of intracel-lular biological processes.
PL
Artykuł przedstawia najnowsze trendy w dziedzinie nauk o materiałach i związkach tej dziedziny nauki z naukami o życiu. Prezentuje także potencjalną możliwość wykorzystania komórki jako specyficznego sensora rozpoznającego produkty inżynierii materiałowej i nanotechnologii.
EN
This paper informs about the latest trends in th e field of materials science and the relationships of this field of science with life sciences. It also presents the potential of using a cell as a specific sensor that recognizes the products of materials engineering and nanotechnology.
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