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Simultaneous quantification of five lignans from Schisandra chinensis in various tissues of rats

Su Lian-Lin, Cheng Xue, Ding Xi-Yan, Mao Chun-Qin, Lu Tu-Lin, Hao Min, Li B.-W., Qin Sirui

Acta Chromatographica

2019

Vol. 31, no. 2

113--119

In this research study, a rapid, sensitive, and specific high-performance liquid chromatography–electrospray ionization–tandem mass spectrometry (HPLC–ESI–MS/MS) method was established and validated, in regard to the simultaneous quantification of five sedative and hypnotic lignans (schisandrin, schisandrol B, schisantherin A, deoxyschisandrin, and schisandrin B) in various tissues of rats (including heart, liver, spleen, lung, and kidney). The purpose of the study was to clarify the tissue distribution of the total lignans extract of Schisandra chinensis (SC). Then, the analytes were separated on a MERCK Purospher STAR LP C18 column (250 mm × 4.6 mm, 5 μm), with a mobile phase consisting of 0.05% (v/v) formic acid acetonitrile, and 0.05% (v/v) formic acid water, and a flow rate of 1 mL/min. All of the calibration curves of the five components showed good linearity (r > 0.9950), with ranges of 4.8 to 1920 ng/mL for analytes. The intra-day and inter-day precisions (relative standard deviation [RSD] %) were within 13.76% for all of the analytes. The average recoveries of the five analytes were greater than 85.23%, and the mean value of the matrix effect ranged from 82.3% to 93.4%. The five analytes were confirmed to be stable during the storage, preparation, and analytic procedures. The major target tissues of the total lignans extract of the SC in the rats were the livers and kidneys.

Topographic differences play a limited role on species distribution in a sub-tropical forest in South China

Gong G.-Q., Huang Z.-L., Ye W.-H., Cao H.-L., Lian J.-Y., Lin G.-J., Wei S.-G., Li L., Bin Y., Kong B., Li B.-W.

Polish Journal of Ecology

2013

Vol. 61, nr 3

477--491

Habitat association test has been regarded as an effective way to evaluate the relative role of niche differentiation in a community. Topographical attributes are often selected as surrogates of niche processes. However, the inference for relative role of niche differentiation is likely problematic if the spatial dependence of plant community on underlying spatially structured topographic variables is not accounted for. In this study, we used the torus shift and principal component analysis (PCA) to resolve this problem, and tested the habitat associations by adjusted-SD test in a sub-tropical evergreen broad-leaved forest in south China. The result revealed that minority of 100 common species were specialist, among which 8 species associated with first two PC axes (accounted for 98% of total eigenvalue and contributed mainly by mean elevation and aspect) and 22 species associated with last two PC axes (contributed by slope and convexity). In conclusion, we found the niche processes represented by topographical attributes play a limited role in the Dinghushan sub-tropical evergreen broad-leaved forest plot. Further study should include more habitat variables to examine the habitat associations.