The paper deals with the influence of oils on surface water pollution. Two mineral oils of petroleum origin, one synthetic oil and one vegetable oil were tested. Properties of the selected oil types were assessed by ecotoxicological tests. The acute toxicity test on Daphnia magna showed that out of the tested oils the most toxic for these aquatic organisms were the petroleum oils. In the phytotoxicity test on Sinapis alba, the toxic effect of mineral oils in comparison with synthetic and vegetable oil was more significant. Oils create oil stains visible under the microscope. It was difficult to differentiate them from Scenedesmus subspicatus. The calculation of growth rate was not relevant and a significant loss of cells was detected. It follows from the summary of the tests results that vegetable oil is the least aggressive for the aquatic environment and there are no significant differences between synthetic and mineral oils.
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