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Bioaccumulation of Chromium, Iron, and the Expression of TNF-α and Caspase-3 in Mudskipper (Periophthalmus spp.) from Ambon Island Waters, Indonesia

Liline Sintje, Rumahlatu Dominggus, Salmanu Sriyanti I. A., Pattipeilohy Mery, Sangur Kristin

Journal of Ecological Engineering

2022

Vol. 23, nr 7

90--104

TNF-α protein and caspase-3 in mudskipper muscle can be used as a biomarker to evaluate the environmental conditions. Therefore, this study aimed to analyze the heavy metal contents, specifically Cr and Fe, in the sediments from the mangrove area, as well as the expression of TNF-α and caspase-3 in mudskipper muscle. The samples used were the sediments and mudskippers from the waters of Poka, Waai, and Rutong Villages. The heavy metals were analyzed using Atomic Absorption Spectrophotometry (AAS), while the TNF-α and caspase-3 expressions were assessed with immunohistochemical staining. Observations were carried out using an optical microscope with 1000x magnification. The results showed that the proportion of Fe accumulated in mudskipper at the three locations was greater than Cr, while Cr was higher in the sediment compared to Fe. Moreover, the TNF-α and caspase-3 expressions appeared in muscle organs in varying amounts. This indicates that Cr and Fe under excessive conditions can be toxic to mudskipper causing muscle inflammation and cell death, which leads to the expression of TNF-α and caspase-3.

Mudskipper as an Indicator Species for Lead, Cadmium and Cuprum Heavy Metal Pollution in the Mangrove, Ambon, Indonesia

Sangur Kristin, Leiwakabessy Fredy, Tuaputty Hasan, Tuwankotta Laura Vintan, Samloy Sisilya Vionetha, Ratila Costantina, Salakory Olivia Basye, Matulessy Chimberly, Rumahlatu Dominggus

Journal of Ecological Engineering

2021

Vol. 22, nr 4

1--19

The heavy metal pollution in seawater and sediment can damage mangrove ecosystems and accumulate in mudskipper (Periophthalmus spp.) that lives in mangrove forests. The accumulation of heavy metal in mudskipper can affect the structure of its gill tissue, liver, and muscles. This research aims at (1) analyzing the levels of lead, cadmium and cuprum heavy metals in mudskipper, sediment, and seawater and (2) analyzing the damage to the gill, liver, and muscle tissue of mudskipper due to the exposure to heavy metals. The mudskipper samples were collected from the mangrove forests of Poka, Waai, and Rutong from May to June in 2019. The heavy metal analysis was conducted using the AAS method, while the gill, liver and muscle tissue staining was carried out with the HE (Hematoxylin Eosin) staining method with 40x magnification. The data were analyzed using descriptive analysis and correlational analysis. The results of the analysis showed that based on the mangrove locations, the order of Pb and Cu accumulation in mudskipper was Rutong> Poka> Waai; while the order of the Cd accumulation was Rutong> Waai> Poka. The changes in the gill tissue structure due to the exposure to heavy metals were in the form of teleangiectasia, secondary lamellar edema, hyperplasia, epithelial desquamation, clubbing, and primary lamellar edema. The changes in the liver tissue structure due to the exposure to heavy metals involved hemorrhage and fat degeneration. The changes in the muscle tissue structure due to the exposure to heavy metals include changes in muscle fibers, edema, and necrosis. The highest accumulation of Pb and Cd was found in gills and muscles from seawater, while low level of heavy metal Cu was found in the liver.