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Study of the Conditions for Accelerating the Composting Process when Adding Microbial Communities

Sokolova Valeriia, Krusir Galina, Sagdeeva Olga, Gnizdovskyi Oleksandr, Malovanyy Myroslav

Journal of Ecological Engineering

2021

Vol. 22, nr 3

11--17

The study of the composting process is a very urgent task. The rapid growth in food production leads to the formation of waste, which should be safely disposed of in a natural way – composting. Composting the vegetable component of food waste can significantly reduce the proportion of waste falling into landfills and utilize the food waste from the hotel and restaurant industry. Additional enrichment of the compost mixture of food waste with the «Baikal EM» microbiological additive accelerates the maturation process and enhances the efficiency of the finished compost. Such compost does not contain viable weed seeds or pathogenic microflora and can be used as a fertilizer. In contrast to the control, a sufficient level of germination index is observed for the studied samples and they are not phytotoxic. The finished compost can be used as a fertilizer in agriculture for growing crops. The results obtained indicate that the index of germination of radish seeds increases along with the duration of fermentation of the composted mixture. Communities of microorganisms contained in the «Baikal EM» additive accelerate the natural process of composting, taking an active part in the destruction of the components of the vegetable mixture. The pH value of the finished compost, both under thermophilic and mesophilic conditions, is close to neutral indicators. High values of CO2 emissions from reactors were recorded in the second week under thermophilic and for 3 weeks under mesophilic conditions. The finished compost that fermented under thermophilic and mesophilic conditions can be used as fertilizer, but considering that additional energy is required to create thermophilic conditions, it is advisable to compost the food waste mixture with the addition of the microbiological additive «Baikal EM» under mesophilic conditions.

A Promising Way to Dispose of Fatty Waste by Hydrolysis and Study of the Conditions for Immobilization of Rhizopus Japonicus Lipase on Carriers

Skliar Viktoriia, Krusir Galina, Shunko Hanna, Zakharchuk Valentyna, Malovanyy Myroslav

Journal of Ecological Engineering

2020

Vol. 21, nr 2

201--208

The hydrogenation process in oil and fat production is accompanied by the formation of a large amount of waste, the majority of which includes spent catalysts and spent sorbents. The bioconversion of lipids through the use of immobilized enzyme preparations expands the possibilities and is one of the most powerful resource potentials of environmental biotechnology. The adsorption methods are most effective, which is due to the ease of the binding process, the low cost of the carrier and the absence of toxic substances. Immobilization of adsorption provides a large surface area, which is important for the lipolytic enzymes performing catalysis at the interface. In most cases, adsorption slightly reduces the activity of lipases and, which is extremely important, does not affect their specificity. It was shown that the use of activated carbon with a grain size of 2.0–2.8 as a carrier for immobilization of lipase, leads to maximum preservation of the initial lipolytic activity. The weight ratio of carrier/ enzyme, optimal in terms of preservation of lipolytic activity, was 1 g of biopolymer carrier per 500 mg of lipase (1: 0.5) with preservation of 36.33% of the initial activity of the native enzyme. From the obtained experimental data, it follows that the rational conditions for the immobilization of Rhizopus japonicus is GM 1.5, temperature 25°С, duration of immobilization 15 minutes, the size of particles of activated carbon as a matrix is 2.0–2.8 mm. The lipolytic activity of the enzyme immobilized under these conditions is preserved by more than 30% compared with the native one, which is a high indicator of the preservation of activity.