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Investigation of human mesenchymal stem cells culture on nanofibrous polyurethane scaffolds and films

Çelebi-Saltık B., Öteyaka M.Ö.

Journal of Achievements in Materials and Manufacturing Engineering

2014

Vol. 64, nr 2

53--61

Purpose: of this study was to evaluate whether electrospun porous nanofibrous scaffold of polyurethane (PU) with low and high beads accommodate the viability and growth of human bone marrow mesenchymal stem cells (hBM MSCs) in comparison with flat surface (Polypropylen). Design/methodology/approach: To our knowledge, the influence of the beads density on nanofibrous scaffold has never been investigated. For this purpose, we electrospun PU to fabric two porous nanofiber scaffolds with less and high density beads to enhance cells attachment and proliferation of hBM MSCs. Moreover, those surfaces were compared to a flat surface (PP). The samples were studied using scanning electron microscopy (SEM), Attenuated Total Reflectance Fourier Transform Infrared (ATR-FTIR) and static contact angle measurement. Findings: The characterization of the samples revealed that hydrophilic surface of high quantity nanofiber with fewer beads scaffolds (LBNF-PU) had less nanofiber with higher quantity of beads that were overlapped on each other firmly compared to low quantity nanofiber with more beads scaffolds (HBNF-PU). MSCs cell morphology on both HBNF-PU and LBNF-PU nanofibrous scaffolds and flat surface was different; it was observed elongated cell shape for LBNF-PU and flat surface and rounded cell shape for HBNF-PU. Live/dead studies confirmed cell viabilities on flat and nanostructured surfaces. Cells expansion on Polypropylen and nanofibrous scaffolds were increased until 7 days of culture. Research limitations/implications: The randomly nanofiber scaffold limited the growth of human bone marrow mesenchymal stem cells (hBM MSCs). The aligned nanofiber scaffold will be evaluated at next investigation. Originality/value: Nanofibrous scaffold have recently draw attention for potential applications in small vascular replacement. Human bone marrow mesenchymal stem cells (hBM MSCs) growth on porous nanofibrous scaffolds is a promising strategy for tissue engineering. The influence of the beads density on nanofibrous scaffold has never been investigated. For this purpose, we electrospun PU to fabric two porous nanofiber scaffolds with less and high density beads to enhance cells attachment and proliferation of hBM MSCs.