Wyniki wyszukiwania - BazTech

Ograniczanie wyników

2 Acta Chromatographica

Znaleziono wyników: 2

Liczba wyników na stronie

Wyniki wyszukiwania

Sortuj według:

Ogranicz wyniki do:

Optimum separation condition of five bioactive compounds by HCI program in HPLC

Tian M., Li S., Row K. H.

Acta Chromatographica

2012

Vol. 24, no. 1

65--74

An efficient method used to separate five bioactive compounds from Gelidium amansii was optimized by the HCI software. The optimum composition of mobile phase for high-performance liquid chromatographic (HPLC) separation was obtained. The elution profiles were calculated by the polynomial theory based on the retention factor ln k = A + BF + CF2 (F was the volume percentage of acetonitrile with 1.0% acetic acid); then, the theory was applied to calculate the elution profile in both isocratic and gradient modes by modifying different mobile phase conditions with HCI program. The calculated results of mobile phase condition suggested that acetonitrile-water (containing 1.0% acetic acid) with a linear gradient elution of 0∼30 min from 15:85 to 50:50 (v/v) was the optimized component. In the experimental conditions, the agreement between the experimental elution profiles and the calculated values of eluted concentration was relatively good.

Retention behaviour of N-CBZ-D-phenylalanine and D-tryptophan: effect of ionic liquid as mobile-phase modifier

Polyakova Y., Row K. H.

Acta Chromatographica

2006

No. 17

210-221

Studies have been performed to investigate the effect of an ionic liquid, 1-butyl-3-methylimidazolium tetrafluoroborate, as mobile-phase modifier on the retention behaviour of N-carbobenzyloxy-D-phenylalanine and D-tryptophan on a reversed-phase silica-based column. Retention of these amino acids was determined with water–acetonitrile mobile phases and with mobile phases containing 2, 5, or 10 mmol L-1 ionic liquid. The effect of the amount of ionic liquid modifier on retention was also studied, and interpreted on the basis of intermolecular interactions. The ionic liquid showed promise as an additive in high-performance liquid chromatography.