The potential use of commercial enzyme and pH control has been investigated for enhancing dairy manure fermentation and modeling dissolved organic matter and orthophosphate (PO4-P) dynamics of fermenters. Anaerobic lab-scale batch fermenters (initial total solids concentration (TSo) = 3.8 wt. %) were fed with separated dairy manure solids and operated under pH controls (5 and 9.5). The enzyme-supplemented alkaline fermenters clearly outperformed the acidic fermenters in terms of chemical oxygen demand (COD) solubilization: ca. 50% vs. 20%, respectively. Soluble PO4-P in the acidic fermenters was comparably higher but constituted less than 20% of total phosphorus. Better soluble COD (>80%) and soluble PO4-P (>70%) yields were noted for the dilute fermenters (TSo = 0.6–0.8 wt. %). An existing model was retrofitted, calibrated and validated for simulating dynamics of soluble COD, volatile fatty acids, and soluble orthophosphate under various pH and enzyme conditions.
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