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1 2011

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Determination of trace quantities of dutasteride in water and wastewater by solid phase extraction and high-performance liquid chromatography

Wasilewska A., Madajczyk M., Szymański A., Urbaniak W.

Ars Separatoria Acta

2011

No. 8

39-49

Steroid compounds are a kind of pollutants which are increasingly more frequently found in environmental samples. Due to the ever more increasing level of such pollutants and their biological activity, they are of interest to analysts. Our study involved the development of a simple and fast method for the assay of dutasteride in treated industrial waste water using high-performance liquid chromatography. As the steroid concentration in water is much lower than the limit of detection of the UV detector used, the compound assayed in the test water was pre-concentrated using solid-phase extraction. Our procedure has high accuracy and a precision of 2.6%, and the recovery of the analyte from water is 78.6%. The procedure enables the assay of dutasteride at a level of 2 microg/L in water.