Aspergillus niger isolated from palm dates was examined to produce citric acid. The isolates from zahdi dates (A. niger S11) had the best yield of citric acid with a straightforward and efficient isolation technique without the need for purification of the isolates. The best results of citric acid obtained in the static air, using Czapek dox liquid medium, were 37.116 g/l, 33.742% yield, and 0.0859 g/L•h productivity, and in the 1 vvm air were 47.248 g/L, 42.953% yield, and 0.1094 g/L•h productivity, under temperature 30 °C, mixing speed 150 rpm, pH 4, sugar concentration 11%, and inoculum size 5%. Yield and concentration were 23.139% and 25.453 g/L using Date syrup.
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