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Simultaneous determination of four major constituents in Citri Grandis Exocarpium by HPLC-DAD

Yu X. X., Liu Q. D., Wu J. W., Liang Z. K., Zhao M. Q., Xu X. J.

Acta Chromatographica

2016

Vol. 28, no. 1

129--143

Citri Grandis Exocarpium (CGE) is a traditional Chinese medicine with a variety of biological activities. For efficient quality control of CGE, a simple, rapid, and accurate high-performance liquid chromatographic (HPLC) method was developed for simultaneous determination of four main compounds (naringin, rhoifolin, meranzin hydrate, and isoimperatorin) in this herb. These four compounds were separated on a C18 column by gradient elution with methanol and water. The flow rate was 1.0 mL•min−1, and the detection wavelength was 324 nm. The recoveries of the method ranged from 96.32% to 103.71%, and good linear relationships (r2 > 0.9998) over relative wide concentration ranges were obtained. Then this validated method was successfully applied to the analysis of nine batches of CGE samples.

Isolation and Purification of Isochlorogenic Acid A from Lonicera japonica Thunb Using High-Speed Counter-Current Chromatography

Yu X. X., Wu J.-W., Liang Z. K., Zhao M., Xu X. J.

Acta Chromatographica

2015

Vol. 27, no. 3

541--549

A high-speed counter-current chromatography (HSCCC) method was established for the isolation and purification of isochlorogenic acid A from Lonicera japonica Thunb. The two-phase solvent system was composed of n-hexane:ethyl acetate: isopropanol:water (2:3:2:5, v/v/v/v). From 150 mg of the ethyl acetate fraction of L. japonica Thunb, 19.65 mg of isochlorogenic acid A was obtained in a one-step HSCCC separation, with a purity of 99.1%, as determined by high-performance liquid chromatography (HPLC). The structure was further identified by ultraviolet (UV), mass spectrometry (MS) and nuclear magnetic resonance (NMR).