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1 2025

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Fluorescencja multispektralna i metody chemometryczne w zastosowaniach (bio)analitycznych

Głowacz Klaudia, Skorupska Sandra, Grabowska-Jadach Ilona, Ciosek-Skibińska Patrycja

Wiadomości Chemiczne

2025

[Z] 79, 11-12

1079--1096

The design of methods based on chemical “fingerprints” (a set of analytical signals) and chemometric tools is an important trend of novel (bio)analysis, aimed at reducing multistep sample preparation and the use of complex analytical instrumentation, while increasing the accessibility of (bio)analytical methods. The excitation-emission matrix (EEM) acquired during the multispectral fluorescence measurements is a potentially rich source of chemical information, serving as a unique, fluorescent "fingerprint" of the sample under investigation. This work summarizes the research on the generation and analysis of chemical “fingerprints”, conducted as part of the doctoral thesis of Klaudia Głowacz, PhD, Eng., under the supervision of Prof. Patrycja Ciosek-Skibińska, PhD, DSc, Eng. The research aimed to develop (bio)analytical methods based on fluorescence excitation-emission spectra and chemometric methods for various applications, including monitoring cell viability in adherent cell cultures, detecting neurotransmitters, amino acids, and oligopeptides, as well as amyloid-β derivatives associated with Alzheimer's disease. This article will discuss in detail the results of research on the development of analytical strategies for selected biological samples – cell cultures – to determine cell viability.