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1
Content available Wettability of poly(ester-ether)s for tissue engineering
Kozłowska A., Piegat A., Fray M. el, Kubies D., Chanova E., Pop-Georgievski O., Rypacek F.
Engineering of Biomaterials
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2010
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Vol. 13, no. 92
5--7
2
Content available Preparation of fibrin networks on polymers containing nano-filler
Kozłowska A., Piegat A., Skrobot J., Strzałkowska D., Fray M. el, Kubies D., Riedel T., Pop-Georgievski O., Rypacek F.
Engineering of Biomaterials
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2010
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Vol. 13, no. 92
8--10
3
Content available Micro- and nanopatterned surfaces for guided adhesion, growth and phenotypic maturation of cells
Bacakova L., Filova E., Grausova L., Vandrovcova M., Parizek M., Novotna K., Svorcik V., Vacik J., Rypacek F., Kromka A., Heitz J., Shard A.
Engineering of Biomaterials
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2009
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Vol. 12, no. 89-91
18--21
EN
Micropatterned surfaces were created by UV light-irradiation of polytetrafluoroethylene through a metallic mask, by successive plasma polymerization of acrylic acid and 1,7-octadiene, or by creation of prominences and grooves by deposition of fullerenes C60 through a metallic mask. All these surface types were capable of inducing regionally-selective adhesion, proliferation and phenotypic maturation of vascular endothelial cells, vascular smooth muscle cells or human bone-derived MG 63 cells. Nanopatterned surfaces created by tethering GRGDSG oligopeptides through polyethylene oxide chains on a polymeric surface promoted spreading, formation of focal adhesion plaques and DNA synthesis in vascular smooth muscle cells. Surfaces nanopatterned with nanocrystalline diamond gave good support for the adhesion, growth and metabolic activity of osteoblast-like MG 63 cells.
4
Content available Adhesion and proliferation of vascular smooth muscle cells on polylactide-polyethylene oxide copolymers with different content and length of polyethylene oxide chains
Filova E., Bacakova L., Lisa V., Kubies D., Machova L., Lapcikova M., Rypacek F.
Inżynieria Biomateriałów
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2004
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R. 7, nr 38-42
19-21
EN
We evaluated antiadhesive effects of polymer surfaces prepared from PDLLA-PEO copolymers using PEO with a different molecular weight and different PEO content in comparison with the native poly(L-lactide) (PLLA) surface. All PDLLA-PEO copolymers significantly decreased number of initially adhered cells (by 23-55% in comparison with pure PLLA) as well as spreading area 24 hours after seeding (by 39-79%). Cell proliferation, estimated by cell number on the 6 day after seeding and bromodeoxyuridine (BrdU) labeling index, was significantly lower on PEO-containing copolymers (by 58-96% and 21-35% respectively) compared to pure PLLA surface. Immunofluorescence staining of vinculin showed that the ability of VSMC to form local adhesion plaques was markedly reduced on surfaces with the highest content of PEO (33 and 44%). Thus, these copolymers are promising for creation of surfaces preventing uncontrolled adsorption of proteins and adhesion of cells. Consecutively, binding of defined ligands for cell adhesion receptors would enable to control cell behaviour on these materials, which could be used for construction of vascular prostheses.
5
Content available Vascular smooth muscle cells in cultures on lactide based polymers for potential construction of artificial vessel wall
Filova E., Bacakova L., Lisa V., Machova L., Lapcikova M., Kubies D., Proks V., Rypacek F.
Inżynieria Biomateriałów
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2003
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R. 6, nr 30-33
9-11
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