Wyniki wyszukiwania - BazTech

1

Electrostatic microencapsulation of living cells

Lewińska D., Bukowski J., Kożuchowski M., Kinasiewicz J., Weryński A.

Biocybernetics and Biomedical Engineering

|

2008

|

Vol. 28, no. 2

69-84

EN

Microencapsulation of different biologically active material for diverse applications have received increasing interest over the last 20 years. Microencapsulation of living cells seems to be a very promising and prospective technology, especially useful in biotechnology and medical applications. One of the most convenient and precise method for this purpose is an electrostatic technique. Electrostatic droplet generation could be performed using single- or multi-nozzle devices, significantly improving efficiency of the process. The usage of an impulse voltage generator allows to manufacture spherical and uniform microbeads with sizes from 0.2 to 3.0 mm of very narrow size distribution. Proposed two-liquid droplet electrostatic formation technique provides preparation of core/shell microbeads, where all cells are immobilized deeply inside a matrix and surrounded with cell-free polysaccharide layer. Such a solution prevents from cell protrusion out of the capsule. Applied electrostatic field is safe for encapsulated living cells and does not cause any cell dysfunction.

2

3D matrigel culture improves differentiated functions of HepG2 cells in vitro

Kinasiewicz J., Kawiak J., Weryński A.

Biocybernetics and Biomedical Engineering

|

2006

|

Vol. 26, no. 4

47-54

EN

In our study, the influence of culture conditions (monolayer and 3D Matrigel) of hepatocyte cell line (buman hepatoma cell line, HepG2) on their metabolic activity was assessed. Albumin synthesis by HepG2 cells was significantly higher in Matrigel culture as compared to monolayer culture of the cells. 3D culture improved urea production by HepG2 cells. The detoxification experiments revealed that HepG2 cells do not metabolize lidocaine neither in monolayer nor in 3D culture. 3D culture significantly improved cell polarity analyzed by F-actin staining and carboxyfluorescein excretion. In conclusion, the 3D Matrigel culture improves highly differentiated hepatic functions of HepG2 cells.

3

The influence of transplantation of encapsulated porcine islets on glycemia in diabetic mice

Kinasiewicz J., Sabat M., Godlewska E., Orłowski T.

Biocybernetics and Biomedical Engineering

|

2004

|

Vol. 24, no. 4

67-70

EN

Transplantation of pancreatic islets is a promising therapy of type I diabetes. Human donor shortage may be alleviated by transplantation of porcine islets. Unfortunately to prevent graft rejection immunosuppresion or immunoisolation is mandatory. In this paper we demostrate, that xenotransplantation of porcine islets microencapsulated in algiate/poly-L-lysine/alginate microcapsules to streptozotocin diabetic mice can reverse hypoglykemia but unfortunatelly the fenomenon of primary graft nonfunction (PNF) can frequently be noted.