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Selection and optimization of staining techniques of Chinese hamster lung fibroblast V79 cell preparations

Rudnicka K., Krzyżewski Z.

Environmental Biotechnology

2011

Vol. 7, No. 2

84-87

We present the results of optimization of fixation and staining of the Chinese hamster lung fibroblast V79 cell line. The experiment included four methods of fixation and three methods of staining and enabled the selection of the best visualization technique for V79 cell lines culture. The Schiff method of colouring with Light Green contrasted well the cell nucleus and the cytoplasm, but the staining was light-sensitive and transient. The Giemsa staining made cell preparations suitable for microscopic evaluation, although the difference between the nucleus and the cytoplasm was not always easily recognisable.