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1

Two-stage bacteriophage production process supported by the electromagnetic field

Rakoczy Rafał, Konopacki Maciej, Kordas Marian, Grygorcewicz Bartłomiej

Chemical and Process Engineering

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2022

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Vol. 43, nr 4

491-–506

EN

The practical applications of bacteriophages are associated with the problems related to the intensification, optimization of process production of this biomaterial and the search for new methods of production. The production of bacteriophages requires a fine balance between the dynamic growth of the bacteriophage and the host. The electromagnetic field (EMF) is a promising biotechnological method for the process production of bacteriophages. This study evaluates the use of various types of EMF to enhance the process. It was found that the process production of bacteriophages is divided into two stages. In the first stage, the influence of various types of EMF on the proliferation process of bacteria (host) was analyzed. Secondly, the process production of bacteriophage was implemented for the optimal infection conditions under the action of the various types of EMF. Moreover, the study demonstrated that the most effective bacteriophage production was the process with the application of the rotating magnetic field (RMF), pulsed magnetic field (PMF) and the static magnetic field (SMF) with negative polarity.

2

Growth optimization of Klebsiella pneumoniae in magnetically assisted bioreactor

Konopacki Maciej, Augustyniak Adrian, Grygorcewicz Bartłomiej, Dołęgowska Barbara, Kordas Marian, Rakoczy Rafał

Chemical and Process Engineering

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2022

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Vol. 43, nr 3

289–-304

EN

In recent years, infections are more often caused by pathogens with high multi-drug resistance, classified as the “ESKAPE” microorganisms. Therefore, investigation of these pathogens, e.g., Klebsiella pneumoniae, often requires biomass production for treatment testing such as antibiotics or bacteriophages. Moreover, K. pneumoniae can be successfully applied as a biocatalyst for other industrial applications, increasing the need for this bacteria biomass. In the current study, we proposed a novel magnetically assisted bioreactor for the cultivation of K. pneumoniae cells in the presence of an external alternating magnetic field (AMF). High efficiency of the production requires optimal bacteria growth conditions, e.g., temperature and field frequency. Therefore, we performed an optimization procedure using a central composite design for these two parameters in a wide range. As an objective function, we utilized a novel, previously described growth factor that considers both biomass and bacteria growth kinetics. Thus, based on the response surface, we could specify the optimal growth conditions. Moreover, we analysed the impact of the AMF on bacteria proliferation, which indicated positive field frequency windows, where the highest stimulatory effect of AMF on bacteria proliferation occurred. Obtained results proved that the magnetically assisted bioreactor could be successfully employed for K. pneumoniae cultivation.

3

Dynamic modelling of bacteriophage production process

Konopacki Maciej, Grygorcewicz Bartłomiej, Gliźniewicz Marta, Miłek Dominika, Kordas Marian, Rakoczy Rafał

Chemical and Process Engineering

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2022

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Vol. 43, nr 4

471–-482

EN

Bacteriophages, viruses that can infect bacteria, are promising alternatives for antibiotic treatment caused by antibiotic-resistant bacteria strains. For that reason, the production of bacteriophages is extensively studied. Mathematical modelling can lead to the improvement of bioprocess by identification of critical process parameters and their impact on the demanded product. Dynamic modelling considers a system (i.e. bioreactor or bioprocess) as a dynamic object focusing on changes in the initial and final parameters (such as biomass concentration and product formation) in time, so-called signals and treats the studied system as a “black box” that processes signals. This work aimed to develop a mathematical model that describes bacteriophage production process. As result, we created a dynamic model that can estimate the number of bacteriophages released from cells as plaque-forming units at specific time points based on the changes in the bacteria host-cell concentration. Moreover, the proposed model allowed us to analyze the impact of the initial virus concentration given by multiplicity of infection (MOI) on the amount of produced bacteriophages.

4

Heat transfer investigations in a liquid that is mixed by means of a multi-ribbon mixer

Borowski Tomasz, Sołoducha Dawid, Musik Daniel, Wójcik Krzysztof, Chyla Mariusz, Ziętarska Katarzyna, Friedrich Małgorzata, Kiełbus-Rąpała Anna, Kordas Marian, Konopacki Maciej, Rakoczy Rafał

Polish Journal of Chemical Technology

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2021

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Vol. 23, nr 2

66--72

EN

The objective of this paper is to present the investigations of the heat transfer process carried out by means of the multi-ribbon mixer. It is shown that the heat transfer process for the synergic effect of the mixing process and the flowing liquid through the mixer has significantly higher values of the heat transfer coefficients than the mixer with motionless impellers. The empirical correlations between the heat transfer coefficient and the operational parameters obtained in this work can provide guidance for the design and operation of an apparatus equipped with the multi-ribbon impeller. These empirical correlations can be used to predict the heat transfer coefficient for the multi-ribbon mixer.

5

Studies of a mixing process induced by a rotating magnetic field with the application of magnetic particles

Rakoczy Rafał, Kordas Marian, Markowska-Szczupak Agata, Konopacki Maciej, Augustyniak Adrian, Jabłońska Joanna, Paszkiewicz Oliwia, Dubrowska Kamila, Story Grzegorz, Story Anna, Ziętarska Katarzyna, Sołoducha Dawid, Borowski Tomasz, Roszak Marta, Grygorcewicz Bartłomiej, Dołęgowska Barbara

Chemical and Process Engineering

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2021

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Vol. 42, nr 2

157-–172

EN

We demonstrate in this study that a rotating magnetic field (RMF) and spinning magnetic particles using this kind of magnetic field give rise to a motion mechanism capable of triggering mixing effect in liquids. In this experimental work two mixing mechanisms were used, magnetohydrodynamics due to the Lorentz force and mixing due to magnetic particles under the action of RMF, acted upon by the Kelvin force. To evidence these mechanisms, we report mixing time measured during the neutralization process (weak acid-strong base) under the action of RMF with and without magnetic particles. The efficiency of the mixing process was enhanced by a maximum of 6.5% and 12.8% owing to the application of RMF and the synergistic effect of magnetic field and magnetic particles, respectively

6

Mathematical modeling of ethanol production by Saccharomyces cerevisiae in batch culture with non-structured model

Konopacka Anna, Konopacki Maciej, Kordas Marian, Rakoczy Rafał

Chemical and Process Engineering

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2019

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Vol. 40, nr 3

281–-291

EN

In this study, batch fermentation of glucose to ethanol by Saccharomyces cerevisiae (ATCC 7754) was carried out using 2.5 dm3 BioFlo®115 bioreactor. The main objective of this study was to investigate the kinetics of ethanol fermentation by means of the non-structured model. The fermentation process was carried out for 72 h. Samples were collected every 4 h and then yeast growth concentration of ethanol and glucose were measured. The mathematical model was composed of three equations, which represented the changes of biomass, substrate and ethanol concentrations. The mathematical model of bioprocess was solved by means of Matlab/SimulinkTM environment. The obtained results from the proposed model showed good agreement with the experimental data, thus it was concluded that this model can be used for the mathematical modeling of ethanol production.

7

Intensification of free-convection heat transfer in magnetically assisted bioreactor

Konopacki Maciej, Kordas Marian, Rakoczy Rafał

Chemical and Process Engineering

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2019

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Vol. 40, nr 3

293-–304

EN

The effect of rotating magnetic field on the heat transfer process in a magnetically assisted bioreactor was studied experimentally. Experimental investigations are provided for the explanation of the influence of the rotating magnetic field on natural convection. The heat transfer coefficients and the Nusselt numbers were determined as a function of the product of Grashof and Prandtl dimensionless numbers. Moreover, the comparison of the thermal performance between the tested set-up and a vertical cylinder was carried out. The relative enhancement of heat transfer was characterized by the rate of the relative heat transfer intensification. The study showed that along with the intensity of the magnetic field the heat transfer increased.