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A simple and sensitive RP-HPLC method for simultaneous estimation of torsemide and spironolactone in combined tablet dosage form

Deshpande P. B, Gandhi S. V., Gaikwad N. V, Khandagle K. S

Acta Chromatographica

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2012

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Vol. 24, no. 1

15--22

EN

Torsemide (TSM) is a loop diuretic used in the treatment of edema, and spironolactone (SPL) is a potassium-sparing diuretic used in hypokalemia. Both are potential diuretics used in combination to treat congestive heart failure. This paper describes a simple, sensitive, accurate, and validated reverse-phase high-performance liquid chromatographic (RP-HPLC) method for the simultaneous quantification of these compounds as the bulk drug and in tablet dosage forms. Separation was carried out on Jasco HPLC system equipped with Neosphere C8 column (150 × 4.6 mm i.d.) and UV/VIS detector using acetonitrile:10 mM potassium dihydrogen phosphate buffer in ratio of 60:40 (υ/υ) as the mobile phase, and detection was carried out at 240 nm. Results were linear in the range of 2–12 μg mL-1 for TSM and 5–30 μg mL-1 for SPL. The method was successfully applied for the analysis of drugs in pharmaceutical formulation. Results of the analysis were validated statistically and by recovery studies.

2

A validated densitometric TLC method for analysis of cefuroxime axetil and potassium clavulanate in combined tablet dosage forms

Sengar M. R, Gandhi S. V., Patil U. P., Rajmane V. S, Bothara K. G.

Acta Chromatographica

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2010

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Vol. 22, no. 1

91--97

EN

A simple, specific, accurate, and precise high-performance thin-layer chromatographic method for analysis of cefuroxime axetil and potassium clavulanate in a combined tablet dosage form is reported. The compounds were separated on aluminium foil plates precoated with silica gel 60 F 254 , with chloroform-methanol-toluene 4:3:3 ( v / v ) as mobile phase. Densitometric evaluation of the separated bands was performed at 225 nm. The two drugs were satisfactorily separated with R F 0.77 ± 0.0114 and 0.29 ± 0.0114 for cefuroxime axetil and potassium clavulanate, respectively. Response was a linear function of amount over the calibration ranges 500–2500 and 2000–10 000 ng per band, respectively. The method was successfully validated and used for analysis of the drugs in a pharmaceutical formulation. Recovery was 100.05 ± 0.98% for cefuroxime axetil and 99.94 ± 0.538% for potassium clavulanate (mean ± RSD).

3

A validated densitometric method for analysis of aceclofenac and paracetamol as the bulk drugs and in combined tablet dosage forms

Gandhi S. V., Barhate N. S., Patel B. R., Panchal D. D., Bothara K. G.

Acta Chromatographica

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2008

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Vol. 20, no. 2

175-182

EN

A simple, specific, accurate, and precise high-performance thin-layer chromatographic method for analysis of aceclofenac and paracetamol in combined tablet dosage forms is reported in this paper. The method uses aluminium plates coated with silica gel 60 F254 as stationary phase and ethyl acetate-n-butanol-glacial acetic acid 7.5:2.5:0.005 (v/v) as mobile phase. Densitometric evaluation of the separated bands was performed at 270 nm. The two drugs were satisfactorily resolved with RF values 0.29 š 0.019 and 0.74 š 0.025 for aceclofenac and paracetamol, respectively. The respective calibration plots were linear over the ranges 50-1000 and 200-1500 ng per band. Intra-day variation, as RSD (%), was 0.420 š 0.282 for aceclofenac and 0.354 š 0.212 for paracetamol. Interday variation, as RSD (%) š SE, was 0.57 š 0.41 for aceclofenac and 0.90 š 1.09 for paracetamol. The method, which was validated in accordance with ICH guidelines, can be used for analysis of ten or more formulations on a single plate and is a rapid and costeffective quality-control tool for routine simultaneous analysis of aceclofenac and paracetamol in combined dosage forms.