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Determination of the fatty acids in fish tissue and feed — comparison of different methods and statistical evaluation

Trbović D., Polak T., Demšar L., Parunović N., Dimitrijević M., Nikolić D., Đorđević V.

Acta Chromatographica

2018

Vol. 30, no. 3

175--179

With the aim to reinforce laboratory competence in the field of testing the quality of fish from aquaculture, a study on the precision of fatty acid (FA) analyses in fish meat and fish feed was undertaken. Different methods were performed in laboratories. In situ transesterification method and extraction of lipids from the fish were followed by capillary gas chromatography with flame ionization detection. The reproducibility (R) values of the majority of FAs were less than 3% of their absolute values. Differences in calculating ionization detector response factors and/or autoxidation caused by faulty sample-handling could lead to variation in quantification of FAs in fish, especially for FA C22:6n-3. Statistical analysis showed a significant correlation between the two laboratories' quantifications of FAs in fish and fish feed (Pearson's correlation coefficient; r = 0.987, r = 0.994, and r = 0.997; for fish Z [trout], fish Š [rainbow trout], and fish feed, respectively). Overall, adequate accuracy was obtained in this study. The proposed method provides a fast and efficient means of identifying fish and feed for quality control purposes.

Optimum conditions for copper extraction from the flotation waste using factorial experimental design

Mihajlovic I., Štrbac N., Dordević P., Mitovski A., Nikolić D., Zivkovic Z.

Environment Protection Engineering

2012

Vol. 38, nr 4

171--184

Copper flotation waste, generated as a byproduct of pyrometallurgical copper production, contains a significant amount of Cu together with trace amounts of other toxic elements such as Fe, Sn, Sb, As, and Pb. It is usually disposed in uncovered tailing ponds in the vicinity of a copper smelter plant. Heavy metals released into the water and soil can cause a number of environmental problems. The amount of copper in copper flotation waste is high enough to be extracted economically using appropriate methods. In this study, the leaching characteristics of copper flotation waste from the Bor Copper Mine, Serbia, were investigated to assess the feasibility of copper extraction.