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The objective of this paper was to evaluate the effects of high temperatures on biochemical parameters of hens. Heat stress generally causes a decrease in feed intake. The hypothesis that feed intake, egg production, egg quality measures, acid-base status (pH, pCO2, HCO3 –) of hens would be influenced by the high temperature period was tested. Twenty four Isa Brown hens at 60 weeks of age were used in this experiment and hens were randomly separated into 2 equal groups. Each group contains 12 hens. First control group was housed at 20.1÷21.2°C and 60÷65% relative humidity. Experimental group was housed for 2 weeks at 20.5°C and 60÷65% relative humidity in experimental box, and then exposed to 27°C and 60% for 4 week, and then allowed to recover for 2 week at 20.5°C and 60% relative humidity. All production parameters and egg quality were significantly affected by heat stress. Feed intake during first week heat period was significantly reduced (18%). In three next weeks, feed intake was increasing with reduction 14.5%, 8% and 9.5%, respectively. Blood pH values were significantly lower from 3rd week of exposure. Blood pH had these values: 1st week 7.372, 2nd week 7.371, 3rd week 7.380 and 4th week 7.289.
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