Przedstawiono wyniki rozpoznania hydrograficznego i hydrochemicznego siedmiu grup wypływów wód podziemnych w okolicach Lęborka. Punktowe wypływy występują skupiskowo - w systemach owalnych nisz, zalesionych zboczy dolin rzecznych, w obrębie rozcięć erozyjnych, często o charakterze antropogenicznym. Cechy chemiczne wód źródlanych nawiązują do rodzaju skał zlewni, a zwiększo¬ne stężenia azotu azotanowego w części z nich są związane z zagospodarowaniem zlewni.
EN
This paper presents the results of hydrographic and hydrochemical Identification of seven groups of underground water outflows in the area of Lębork. The outflow points occur in clusters - in oval niche systems, on wooded slopes of river valleys, within the bounds of erosion dissections, frequently of anthropogenic origin. Chemical characteristics of spring waters are related to the type of rocks occuring in the catchment basin, and increased concentrations of nitrate nitrogen in some of them are a consequence of the catchment basin development.
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The research presented here was conducted in order to determine the influence of exogenous traumatic acid (TA) on the growth, metabolism, and antioxidative activity of vascular water plant, Wolffia arrhiza Wimm. The research was concerned with TA influences, in concentrations of 10^-8 M – 10^-4 M, on fresh W. arrhiza, and on primary metabolites, such as monosaccharides, proteins, chlorophylls a and b, and carotenoids. It was determined that TA causes a substantial increase in these metabolites compared to the control, especially at concentrations of 10^-7 – 10^-6 M. Polyacrylamide gel electrophoresis of proteins (SDS-PAGE) was conducted in order to specify in more detail the qualitative changes in proteins, whose synthesis is stimulated by TA. Under the influence of TA W. arrhiza cells saw an induction of de novo synthesis of 3 proteins with molecular weights of 10, 58, and 90 kDa. It was proven that 10^-7 – 10^-6 M concentrations of TA also increase photosynthesis intensity and the activity of antioxidative enzymes (ascorbate peroxidase, catalase, glutathione reductase, and superoxide dismutase). In cells treated with exogenous TA, lipids' peroxidation decreases (expressed as a drop in malonyl dialdehyde) and sulfhydryl (SH) groups in proteins increase. Based on our research, TA plays an important role in the regulation of growth and metabolism in W. arrhiza. Our results also show that TA possibly participates in the activation of antioxidant enzymes and its probable participation in the metabolic responses of lower water plants to oxidative stresses.
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The aim of this work is to detect how 3,5-diacetamido-2,4,6-triiodosodium benzoate (sodium amidotrizoate), a synthetic derivative of the anti-auxin triiodonezoic acid (TIBA), influences the growth and content of primary metabolites in the green alga Chlorella vulgaris Beijerinck. It strongly suppressed cell number by 6–20%, chlorophyll a by 6–14%, chlorophyll b by 20–24%, total carotenoids by 7–13% and monosaccharides by 7–8% at the concentration of 10^-4 M, in comparison to the control. On the other hand, sodium amidotrizoate applied at lower concentrations (10^-7 – 10^-6 M) acted as a weak stimulator of analyzed biochemical compounds in algal cells. No statistically significant effect was detected with the application of sodium amidotrizoate at a concentration of 10^-8 M. It seems that sodium amidotrizoate plays an important role in algal growth and development and probably possesses anti-auxin activity, like TIBA.
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The effect of exogenous traumatic acid, (2E)-dodecene-1,12-dioic acid (TA), at concentrations of 10-6 - 10-3 M on the growth and development of the unicellular green alga Chlorella vulgaris was examined over 7 days. TA plays an important role in algal growth and development. Treatment with 10-5 M TA resulted in the greatest increase in cell numbers, by 77%, chlorophyll a content, by 126%, chlorophyll b, by 45%, total carotenoids, by 65%, monosaccharides, by 111%, and water-soluble proteins, by 37%, in relation to the control. After three days of treatment with 10-5 M TA cultures were analysed by SDS-PAGE, which showed the presence of new polypeptides with molecular weights 13-103 kDa.
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