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Evaluation of the microbial, cytotoxic and physico-chemical properties of the stainless steel crowns used in pediatric dentistry

Sztyler Klaudia, Pajączkowska Magdalena, Nowicka Joanna, Rusak Agnieszka, Chodaczek Grzegorz, Nikodem Anna, Wiglusz Rafał J., Watras Adam, Dobrzyński Maciej

Acta of Bioengineering and Biomechanics

2022

Vol. 24, no. 4

127--137

Preformed stainless steel crowns are used in pediatric dentistry to obtain full crown restoration of primary molar teeth. They are consider the best restoration in terms of durability and effectiveness. The purpose of this study is to evaluate microbial, cytological and physio-chemical properties to determine whereas stainless steel crown are biocompatible, safe for surrounding tissue and helpful in avoiding micro-organisms influence on the tooth tissue. Based on the results, it was determined that stainless steel crowns used in pediatric dentistry represent no cytotoxic risk to the surrounding tissues, have a low probability of developing hypersensitivity to the coronal material and also that their biological properties make them suitable to use in pediatric dentistry for the reconstruction of damaged primary molar tissue.

Assessment of cytotoxic and antimicrobial activity of selected gingival haemostatic agents – in vitro study

Szymonowicz Maria, Rusak Agnieszka, Pajączkowska Magdalena, Nowicka Joanna, Wiśniewska Kamila, Żywicka Bogusława, Żywicka Bogusława, Rybak Zbigniew, Dobrzyński Maciej

Acta of Bioengineering and Biomechanics

2020

Vol. 22, no. 3

185--198

Purpose: The present research aimed to determine whether and how the aluminium chloride – based materials affect the cell line of the bacterial line and fungi. Methods: Cytotoxicity of haemostatic astringents: Alustat (liquid), Alustat (gel), Alustat (foam), Alustin, Hemostat, Racestyptine and Traxodent containing AlCl3 was conducted on L929 cell line with the use of MTT and SRB assays. The antimicrobial activity (CFU and MIC) against C. albicans, S. mutans, L. rhamnosus was determined. Results: In the MTT results, cell viability for all agents were very low. In SRB, the lowest cytotoxicity was demonstrated for Hemostat and Alustat (foam), Traxodent and Racestyptine. Total reduction of the CFU of S. mutans was observed. Alustat (gel) and Alustat (liquid) completely inhibited the growth of C. albicans, S. mutans and L. rhamnosus. Conclusions: The viability of L929 cells obtained in the SRB assay is more reliable than that obtained in the MTT assay, in the case of gingival haemostatic agents.