Wyniki wyszukiwania - BazTech

Ograniczanie wyników

2 Journal of Ecological Engineering

Znaleziono wyników: 2

Liczba wyników na stronie

Wyniki wyszukiwania

Sortuj według:

Ogranicz wyniki do:

Comparative Testing of Multibioagent Inoculants to Control Bipolaris spicifera R15 on Rice Plant

Ali Hamdia Z., Abdulrahman Abdulrahman A., Abdullah Ali A., Saood Hutham M.

Journal of Ecological Engineering

2021

Vol. 22, nr 11

168--177

The present research deals with greenhouse studies on the efficacy of Cladosporium halotolerans CIR 18_ITS and Meyerozyma guilliermondii MIR 15_ITS compared with a compatible Trichoderma isolate T.4679 to control the phytopathogenic Bipolaris spicifera R15 fungus. An experiment was carried out under controlled conditions in a greenhouse with sterilised soil, and 13 parameters were evaluated. The greenhouse results triggered significant differences [p<0.05] on rice plants after two-month post planting in all treatments compared with the untreated control due to pre-inoculation with three multibiocontrol agents. In addition, results showed the significant interaction amongst three multibiocontrol agents on the growth parameters of the rice plant, fresh weight of shoot and root, dry weight of shoot, root, shoot and root length and greater efficiency of reducing disease severity when treated with the Trichoderma isolate T.4679, M. guilliermondii MIR 15_ITS and C. halotolerans CIR 18_ITS individually or in combination with each other. The greenhouse experiment exhibited that C. halotolerans CIR 18_ITS alone, M. guilliermondii MIR 15_ITS alone, C. halotolerans CIR 18_ITS + C. halotolerans CIR 18_ITS and M. guilliermondii MIR 15_ITS + Trichoderma isolate T.4679 + C. halotolerans CIR 18_ITS + C. halotolerans CIR 18_ITS have greater efficiency of reducing disease infection and severity by approximately 11.11% and 6.67%, respectively, amongst all treatments mentioned.

First Report on Fusarium brachygibbosum Isolate FIR 16_ITS Isolated from Iraqi Wheat Plant

Ali Hamdia Z., Hameed Mahdi S., Abdulrahman Abdulrahman A., Saood Hutham M.

Journal of Ecological Engineering

2020

Vol. 21, nr 3

81--86

In this study, the Fusarium Brachygibbosum fungus isolate FIR 16_ITS was isolated for the first time from the infected wheat plants in Iraq, during the winter of 2018, in the AL-Tewatha region – 20 km south east of Baghdad. In order to verify the identity of the species of the F. brachygibbosum isolate FIR 16_ITS with the growth symptoms on the wheat plants, the wheat seeds and tissue plants were obtained and used as a source for the re-isolation of the infection agent. F. brachygibbosum isolate FIR 16_ITS was hardly identified using visual approach. However, a molecular technique is important in verifying the species of F. brachygibbosum isolate FIR 16_ITS. The isolate obtained was used for the extraction of DNA and later used for the molecular identification and phylogenetic analyses based on rDNA-internal transcribed region (ITS) primer sets. The authors successfully obtained the amplified products of the ITS-rDNA region, and a sequence analysis indicated that the Fusarium sp. isolate FIR 16_ITS species screened in this study belonged to the F. brachygibbosum species. It was recorded in the NCBI under the following information NCBI; BioSample; Accession no.; SAMN11408139; ID: 11408139 and NCBI; BioProject; Accession no.; PRJNA532637; ID: 679434 (Locus Tag Prefix; E7750).