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1
Content available remote Biphasic monolithic osteochondral scaffolds obtained by diffusion-limited enzymatic mineralization of gellan gum hydrogel
Pietryga Krzysztof, Reczyńska-Kolman Katarzyna, Reseland Janne E., Haugen Håvard, Larreta-Garde Véronique, Pamuła Elżbieta
Biocybernetics and Biomedical Engineering
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2023
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Vol. 43, no. 1
189--205
EN
Biphasic monolithic materials for the treatment of osteochondral defects were produced from polysaccharide hydrogel, gellan gum (GG). GG was enzymatically mineralized by alkaline phosphatase (ALP) in the presence of calcium glycerophosphate (CaGP). The desired distribution of the calcium phosphate (CaP) mineral phase was achieved by limiting the availability of CaGP to specific parts of the GG sample. Therefore, mineralization of GG was facilitated by the diffusion of CaGP, causing the formation of the CaP gradient. The distribution of CaP was analyzed along the cross section of the GG. The formation of a CaP gradient was mainly affected by the mineralization time and the ALP concentration. The formation of CaP was confirmed by Fourier transform infrared spectroscopy (FTIR), Raman spectroscopy and mapping, as well as energy-dispersive X-ray spectroscopy (EDX) mapping of the interphase. The microstructure of mineralized and non-mineralized parts of the material was characterized by scanning electron microscopy (SEM) and atomic force microscopy (AFM) showing sub-micrometer CaP crystal formation, resulting in increased surface roughness. Compression tests and rheometric analyzes showed a 10-fold increase in stiffness of the GG mineralized part. Concomitantly, micromechanical tests performed by AFM showed an increase of Young’s modulus from 17.8 to more than 200 kPa. In vitro evaluation of biphasic scaffolds was performed in contact with osteoblast-like MG-63 cells. The mineralized parts of GG were preferentially colonized by the cells over the non-mineralized parts. The results showed that osteochondral scaffolds of the desired structure and properties can be made from GG using a diffusion-limited enzymatic mineralization method.
2
Content available Poly(sebacic anhydride) microparticles loaded with curcumin for pulmonary purposes
Kwiecień Konrad, Reczyńska-Kolman Katarzyna, Niewolik Daria, Jaszcz Katarzyna, Pamuła Elżbieta
Engineering of Biomaterials
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2021
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Vol. 24, no. 162
7--12
EN
Microparticles (MPs) made of fast biodegrading biomaterials, loaded with drugs, are considered a superior treatment method for pulmonary infections. One of the promising biomaterials for obtaining such a drug delivery system (DDS) is poly(sebacic anhydride) (PSA) due to its favourable degradation kinetics and mechanism. In this paper, we present a study of manufacturing MPs from PSA loaded with curcumin (CU) for pulmonary purposes. MPs were manufactured by oil-in-water emulsification; their morphology and size distribution were evaluated using optical microscopy, while the encapsulation efficiency and drug loading were obtained by the fluorometric assay. The cytotoxicity of the MPs, both the empty ones and loaded with CU, was analysed by in vitro tests with BEAS-2B human lung epithelial cells. To this end, metabolic activity by AlamarBlue assay and fluorescent staining (DAPI/ eosin) of the cells were performed. The MPs produced were round, regular in shape with diameters in the range of 1-5 µm and of yellow colour originating from CU. The CU encapsulation efficiency ranged from 42% to 55% and decreased with a higher CU ratio. The drug loading ranged from 4% to 11% and increased at a higher CU ratio. Both empty and CU-loaded MPs did not show a cytotoxic effect at concentrations up to 10 µg/ml.
3
Content available Poly(sebacic anhydride) microparticles loaded with curcumin for pulmonary purposes
Kwiecień Konrad, Reczyńska-Kolman Katarzyna, Niewolik Daria, Jaszcz Katarzyna, Pamuła Elżbieta
Engineering of Biomaterials
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2021
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Vol. 24, no. 163
43
4
Content available Modification of titanium implant surface for animals
Kazek-Kęsik Alicja, Sadkowska Alicja, Reczyńska-Kolman Katarzyna, Kalemba-Rec Izabela, Śmiga-Matuszowicz Monika, Pamuła Elżbieta, Simka Wojciech
Engineering of Biomaterials
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2021
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Vol. 24, no. 163
16
5
Content available Inhalable drug delivery system based on poly(sebacic acid) and azithromycin for the treatment of bacterial infections
Reczyńska-Kolman Katarzyna, Kwiecień Konrad, Knap Karolina, Niewolnik Daria, Kazek-Kęsik Alicja, Płonka Joanna, Jaszcz Katarzyna, Pamuła Elżbieta
Engineering of Biomaterials
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2021
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Vol. 24, no. 163
86
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