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Wykorzystanie chromatografii preparatywnej do wyodrębniania galaktozylowych pochodnych hydroksykwasów

Wojciechowska Aleksandra, Klewicki Robert, Sójka Michał, Klewicka Elżbieta

Przemysł Chemiczny

2020

T. 99, nr 2

293--297

Przedstawiono wyniki badań procesu wyodrębniania galaktozylowych pochodnych hydroksykwasów, uzyskanych w wyniku enzymatycznej galaktozylacji. Wykorzystano kolumnę preparatywną, do której wprowadzano mieszaninę poreakcyjną po wstępnym odsoleniu. W celu oddzielenia sacharydów frakcje z rozdziału chromatograficznego dodatkowo oczyszczano, wykorzystując jonity. Stwierdzono, że metoda ta może być stosowana do pozyskiwania produktów galaktozylacji hydroksykwasów o czystości ok. 80%.

Three hydroxyacids (gluconic, glucoheptonic or ascorbic) were reacted with β-galactosidase at 37°C, pH 6.9-7.0 and for 9 h in presence of an enzyme. The transgalactosilationproducts were sepd. in a column filled with a com. cation exchanger at 40°C and using water as the mobile phase. To obtained saccharide-free products, cationic and anionic exchangers were used. An increase in purity up to 80% was achieved for gluconic and glucoheptonic acid derivatives.

Cell walls polysaccharides of rose hips

Balcerak Bogumił, Milala Joanna, Kosmala Monika, Matysiak Bożena, Klewicka Elżbieta

Biotechnology and Food Science

2019

Vol. 83, nr 2

141--156

The content of alcohol insoluble solids (AIS), polysaccharide composition of cell walls and uronic acids content calculated as galacturonic acid in rose hips (Rosa villosa (Rosa pomifera Herrm) ‘Karpatia', Rosa canina L., Rosa rugosa Thunb.). Rose hips were extracted by means of pectin sequential extraction. Rose achenes contained more alcohol insoluble solids than flesh. The main saccharide building cell walls of rose fruit was glucose from cellulose. The achenes were rich in xylose and the flesh was rich in arabinose. The content of galacturonic acid in flesh was 2 to 3 times higher than in the case of achenes. The largest polysaccharide fraction both in whole fruit and achenes was Concentrated Alkali-Soluble Polysaccharides fraction, while in flesh it was Water Soluble Pectins fraction. Chelating Agent Soluble Pectins fraction was the smallest in the flesh, achenes and the whole hypanthium. Differences in the polysaccharide composition, the content of AIS and galacturonic acid were found, depending on the species of rose hips. In addition, differences in composition were found in the case of wild growing and controlled cultivation hips. Rose hips achenes contain more total dietary fiber than the flesh, but the flesh contains more pectin fraction (soluble dietary fiber) while the achenes contain more cellulose (insoluble dietary fiber).