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Multidimensional TLC procedure for separation of complex natural mixtures spanning a wide polarity range; Application for fingerprint construction and for investigation of systematic relationships within the Peucedanum genus

Cieśla Ł., Skalicka-Woźniak K., Hajnos M., Hawrył M., Waksmundzka-Hajnos M.

Acta Chromatographica

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2009

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Vol. 21, no. 4

641--657

EN

Identification of closely related plant species is not a trivial task, and is often difficult on the basis of morphological or anatomical features. To differentiate among analyzed species, a chromatographic fingerprint is usually constructed. This is not easy for very complex samples, however, especially those containing substances spanning a wide polarity range. In such circumstances more than one fingerprint is constructed, e.g. one for polar and the other for apolar constituents. In this paper a new method has been used for resolution of a mixture of 17 coumarins and flavonoids and, subsequently, construction of a fingerprint of closely related species belonging to the Peucedanum genus. This is the first time multidimensional planar chromatography has been applied for such purpose. Distinctive fingerprints are produced for every analyzed plant species, without the need to construct two fingerprints and without the application of more sophisticated multidimensional methods (e.g. hyphenation of HPLC and TLC, HPLC-MS, etc).

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Computer-assisted searching for coumarins in Peucedanum alsaticum L. and Peucedanum cervaria (L.) Lap.

Skalicka-Woźniak K., Markowski W., Świeboda R., Głowniak K.

Acta Chromatographica

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2009

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Vol. 21, no. 4

531--546

EN

The objective of the work was continuation of our study on identification of target coumarins in extracts from Peucedanum alsaticum L. and Peucedanum cervaria (L.) Lap., by use of ChromSword, on the basis of relationships between retention data and descriptors such as partial molecular volume of structural fragments in water and energies of electrostatic interactions of bond dipoles with water (QSRR). The coumarins were mainly identified by comparing UV spectra from gradient runs with spectra from the literature. The presence of columbianadin, ostruthin, and 8-methoxypeucedanin in the fruits of P. cervaria (L.) Lap. and oxypeucedanin and isoimperatorin in the fruits of P. alsaticum was confirmed. The optimum conditions obtained from DryLab simulation were used for both RP-HPLC and RP-UPLC.

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Variation of the volatile content of the fruits of Peucedanum alsaticum L.

Skalicka-Woźniak K., Łoś R., Głowniak K., Malm A.

Acta Chromatographica

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2008

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Vol. 20, no. 1

119-133

EN

Volatile compounds from fruits of Peucedanum alsaticum L. were isolated by hydrodistillation (HD) and headspace solid-phase microextraction (HS-SPME), and then analyzed by GC-MS. The predominant volatile compounds were α-pinene, sabinene, limonene, and β-phellandrene (more than 60% of the oil). The β-pinene, β-myrcene, camphene, β-caryophyllene, bornyl acetate, and germacrene D content was also high. The in-vitro antibacterial activity of the essential oil and some of its components against ten reference strains of Gram-positive and Gram-negative bacteria was evaluated by the agar dilution method. The essential oil and the compounds were more active against Gram-positive than Gram-negative bacteria.

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Screening for phenolic acids in five species of Iris collected in Mongolia

Machalska A., Skalicka-Woźniak K., Widelski J., Głowniak K., Purevsuren G., Oyun Z., Khishgee D., Urjin B.

Acta Chromatographica

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2008

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Vol. 20, no. 2

259-267

EN

Five species of Iris commonly used in Mongolian traditional medicine (Iris dichotoma Pall., Iris flavissima Pall., Iris bungei Maxim., Iris lactea Pall., and Iris tenuifolia Pall.) were analyzed for the presence of phenolic acids. This was the first study of the phenolic acid content of these species. Samples containing the phenolic acids were prepared by the method of Świątek and then analysed by HPLC with UV-visible diodearray detection (DAD). Identification was performed by comparing retention times with those of standards. Quantitative determination was performed at the absorbance maximum of each phenolic acid (320 nm for ferulic, p-coumaric, and caffeic acids, 280 nm for trans-cinnamic, syringic, and gallic acids, and 254 nm for vanillic, m-hydroxybenzoic, phydroxybenzoic, and protocatechuic acids). As the result of our study ten phenolic acids, both free and liberated by alkaline and acid hydrolysis, were identified by HPLC. Chromatographic investigation revealed the presence of vanillic acid, protocatechuic acid, trans-cinnamic acid, p-hydroxybenzoic acid, p-coumaric acid, ferulic acid, gallic acid, syringic acid, m-hydroxybenzoic acid, and caffeic acid. Quantitative analysis of these acids was also performed. Finally, the presence or absence of some phenolic acids after alkaline or acid hydrolysis was also observed.