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Computer-assisted optimization of liquid-liquid extraction for HPLC analysis of domperidone and pantoprazole in human plasma

Sivakumar T., Manavalan R., Valliappan K.

Acta Chromatographica

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2008

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Vol. 20, no. 4

549-562

EN

This paper deals with optimization of a liquid-liquid extraction procedure for simultaneous HPLC analysis of domperidone and pantoprazole in human plasma. Central composite design and Derringer’s desirability function were used to optimize the concentration of KOH and the volume of ethyl acetate as the main factors affecting the liquid-liquid extraction procedure. After extraction, the analytes were separated quantitatively on a C 18 column with 10 mM pH 7.0 phosphate buffer-methanol-acetonitrile 48.46:20:31.54 ( υ / υ ) as mobile phase at a flow rate of 1.20 mL min -1 and with UV detection at 285 nm. It was concluded that extraction recovery of both the analytes was affected by KOH concentration and that recovery of pantoprazole was affected by ethyl acetate (extraction solvent) volume. Extraction recovery under optimum extraction conditions was 93.52% for domperidone and 92.72% for pantoprazole. The optimized extraction method was validated. Linearity was established for six levels in the ranges 10–1000 ng mL -1 for pantoprazole and 15–1000 ng mL -1 for domperidone. The lower limit of quantitation (LLOQ) and detection (LOD) were estimated as 9.84 and 5.91 ng mL -1 , respectively, for pantoprazole and 14.56 and 8.79 ng mL -1 for domperidone. The optimized method was linear, specific, accurate, and precise; the high recovery (>92%) and low relative standard deviation (<2.5%) enable reliable quantification of these analytes in spiked human plasma.

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Global optimization using Derringer's desirability function: enantioselective determination of ketoprofen in formulations and in biological matrices

Sivakumar T., Manavalan R., Valliappan K.

Acta Chromatographica

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2007

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No. 19

29-47

EN

A reversed-phase high-performance liquid chromatographic method for separation of the enantiomers of ketoprofen in formulations and in plas-ma matrices has been developed and optimized. A central composite design was used to develop response models and Derringer’s desirability function was then used for simultaneous optimization of chiral resolution and analysis time. This procedure enabled discovery of two separate sets of opti-mum conditions for analysis of quality-control samples and plasma samples within the experimental domain. The optimum conditions predicted for quality-control samples were acetonitrile–dipotassium hydrogen phosphate buffer (10 mΜ, pH 6.5)–triethylamine 56:44:0.05 (/ν) as νmobile phase and 1.13 mL min-1 as flow rate. Use of these conditions enabled baseline separation of the enantiomers of ketoprofen in approximately 4.2 min, which is quicker than the previously optimized method. The method was suitable for routine analysis of the enantiomers of ketoprofen in a commercial pharmaceutical preparation and in plasma samples.

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Development and validation of a reversed-phase HPLC method for simultaneous determination of domperidone and pantoprazole in pharmaceutical dosage forms

Sivakumar T., Manavalan R., Valliappan K.

Acta Chromatographica

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2007

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No. 18

130-142

EN

A simple reversed-phase high-performance liquid chromatographic (RP-HPLC) method has been developed and validated for simultaneous determination of domperidone and pantoprazole in capsules. The compounds were separated on an ODS analytical column with a mixture of methanol, acetonitrile, and triethylamine solution (10 mM, pH 7.0 ± 0.05 adjusted with 85% phosphoric acid) in the ratio 20:33:47 (v/v) as mobile phase at a flow rate of 1.0 mL min-1. UV detection was performed at 285 nm. The method was validated for accuracy, precision, specificity, linearity, and sensitivity. The developed and validated method was successfully used for quantitative analysis of Pantop-D capsules. Total chromatographic analysis time per sample was approximately 10 min with pantoprazole, acetophenone (internal standard), and domperidone eluting with retention times of 4.34, 5.52, and 9.46 min, respectively. Validation studies revealed the method is specific, rapid, reliable, and reproducible. Calibration plots were linear over the concentration ranges 0.5–5 µg mL-1 and 1–10 žg mL-1 for domperidone and pantoprazole, respectively. The LODs were 15.3 and 3.0 ng mL-1 and the LOQs were 51.0 and 10.1 ng mL-1 for domperidone and pantoprazole, respectively. The high recovery and low relative standard deviation confirm the suitability of the method for determination of domperidone and pantoprazole in capsules.