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Sensitivity of Nuclear-Cytoplasmic Index and Nuclear- Cytoplasmic Relation in Computer Aided Cytoscreening Diagnosis

Oszutowska-Mazurek D., Mazurek P., Derda K, Sycz K., Waker-Wójciuk G

Przegląd Elektrotechniczny

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2015

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R. 91, nr 2

56-58

EN

Analysis of cytological images stained with the use of Papanicolaou process is important for women health diagnosis. Computer Aided Diagnosis allows fast detection of atypical cells using analysis of area of cell nuclei and corresponding cytoplasm area. Two techniques based on Nucleus-Cytoplasmic Index (NCI), and Nucleus-Cytoplasmic Relation (NCR) are compared. Sensitivity analysis of these methods is provided.

PL

Analiza obrazów cytologicznych wybarwianych metodą Papanicolaou jest istotna w diagnostyce zdrowia kobiet. Komputerowo wspomagana diagnostyka umożliwia szybką detekcję atypowych komórek wykorzystując pole jądra komórki i cytoplazmy. Porównano dwie techniki bazujące na indeksie jądrowo-cytoplazmatycznym (NCI) oraz relacji jądrowo-cytoplazmatycznej (NCR). Przedstawiono analizę wrażliwości metod.

2

From the slit-island method to the Ising model: Analysis of irregular grayscale objects

Mazurek P., Oszutowska-Mazurek D.

International Journal of Applied Mathematics and Computer Science

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2014

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Vol. 24, no. 1

49--63

EN

The Slit Island Method (SIM) is a technique for the estimation of the fractal dimension of an object by determining the area– perimeter relations for successive slits. The SIM could be applied for image analysis of irregular grayscale objects and their classification using the fractal dimension. It is known that this technique is not functional in some cases. It is emphasized in this paper that for specific objects a negative or an infinite fractal dimension could be obtained. The transformation of the input image data from unipolar to bipolar gives a possibility of reformulated image analysis using the Ising model context. The polynomial approximation of the obtained area-perimeter curve allows object classification. The proposed technique is applied to the images of cervical cell nuclei (Papanicolaou smears) for the preclassification of the correct and atypical cells.

3

Analiza wpływu segmentacji jąder komórkowych w rozmazach Papanicolaou na pomiar wymiaru fraktalnego

Mazurek P., Oszutowska-Mazurek D.

Pomiary Automatyka Kontrola

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2012

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R. 58, nr 6

498-501

PL

W artykule badano wrażliwość metody pryzm trójkątnych (TPM - Triangular Prism Method) do estymacji wymiaru fraktalnego dla różnych rozmiarów jądra komórkowego w cytologii ginekologicznej wybarwionej metodą Papanicolaou. Zmiana obszaru analizy pozwala na zmniejszenie wpływu algorytmu segmentacji na wynik. Wykorzystanie kanału zielonego gęstości optycznej i zmian wymiaru fraktalnego dla par skal: 1-2 oraz 2-3 pozwala na otrzymanie wyników dla systemu klasyfikacji z małą wrażliwością na segmentację.

EN

In the paper the influence of segmentation algorithms on estimation for the fractal dimension is analyzed. The Papanicolaou smears are very complex images and their automatic analysis is very hard. Segmentation algorithms of cell nuclei should support blurred and noised edges between cytoplasm and cell nucleus. The estimation of the cell nuclei image parameters is necessary, but the edge related parameters are not sufficient. The classification of the cells (correct/atypical) needs surface related parameters. Fractal based estimators are important for classification. The Papanicolaou images are colourful but only the green channel is important [9]. The TPM (Triangular Prism Method) is applied to the square area (2N+1 edge size) [5]. Multiple box selection variants occur and the multiple TPM analysis is applied and the mean value is calculated. The fractal dimension is calculated for a pair of scales (1-2, 2-3, 3-4). The correct and atypical cell nuclei are known and the analysis is separated. The histograms of difference between the known and reduced cell area are shown (Figs. 6-11). The atypical cells are less sensitive due to a larger size of the analysis area in comparison to the correct ones. Two scales (1-2) and (2-3) are useful, especially for smaller reduction parameter (erosion up to 9 pixels of original cell nuclei). Both scales are used in the classification system [9]. The fractal dimension changes are less than +/- 1%.