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Przygotowanie ekstraktów polisacharydowych pyłku chryzantemy i ich wpływ na komórki in vitro raka jelita grubego

Fang Z., Luo R., Jiang M., Jiang G., Fu M., Chen S., Lai X.

Przemysł Chemiczny

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2018

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T. 97, nr 10

1733--1736

PL

Polisacharydy zawarte w pyłku chryzantem ekstrahowano wodą i wytrącano przy użyciu alkoholu. Cukier o charakterze obojętnym i 2 cukry o charakterze kwasowym rozdzielono, stosując chromatografię jonowymienną. Skład monosacharydów oznaczono metodą wysokosprawnej chromatografii cieczowej. Działanie przeciwnowotworowe polisacharydów oceniano, badając ich wpływ na proliferację komórek nowotworowych. Hamowanie proliferacji HCT116 nie było znaczące, gdy stężenie wynosiło 5 mg/mL (p > 0,05), jednak działanie to w stosunku do komórek nowotworowych HCT116 i HT-29 było znaczące przy tym samym stężeniu (p < 0,05). Nieprzetworzone wyciągi polisacharydowe miały większy wpływ na proliferację komórek in vitro raka jelita grubego niż poszczególne polisacharydy.

EN

Polysaccharides were extd. from Dendranthema indicum var. Asteraceae bee pollens with H2O (yield 7.9%), pptd. with EtOH and sepd. by ion-exchange chromatog. into a neutral sugar and 2 acidic ones. The monosaccharide compn. was detd. by high performance liq. chromatog. They contained rhamnose, galacturonic acid, glucose, galactose and arabinose. The anticancer activity of the polysaccharides was evaluated by studying their effect on proliferation of HCT116 and HT-29 cancer cells. The inhibitory effect of individual sugars on the proliferation of HCT116 was not significant when their concns. were 5 mg/mL, but the total polysaccharides showed a significant effect on proliferation of HCT116 and HT-29 tumor cells at the same concn

2

Preparation and characterization of (Co0:3Zn0:7)(Ti1–xSnx)Nb2O8 microwave dielectric ceramics

Tang B., Zhang X., Fang Z., Liu Q., Zhang S.

Materials Science Poland

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2017

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Vol. 35, No. 2

405--411

EN

Zn0.7Co0.3 (Ti1-xSnx)Nb2O8 (x = 0.1, 0.15, 0.2, 0.25, 0.3, 0.35) microwave ceramics were prepared by traditional solidstate reaction method. The influences of Sn substituted for Ti on the phase constitution, crystal structure and microwave dielectric properties of Zn0.7Co0.3 (Ti1-xSnx)Nb2O8 ceramics were discussed. The XRD patterns revealed the main phase of ZnTiNb2O8 and little content of Zn0.17Ti0.5Nb0.33O2 secondary phase. With further substitution of Sn, the lattice constant, volume and apparent density of the ceramics increased, the ceramic structure reached a maximal compactness at x = 0.2 which was shown on SEM. Tremendous improvement of Q × f and a declining trend of Ɛr and τf were obtained with increasing x value. Appropriate substitution value (x = 0.10) would ensure excellent microwave dielectric properties (Ɛr = 34.1, Q × f = 40562 GHz, τf =-5 ppm/°C) of the ceramics sintered at 1080 °C.

3

Fabrication and properties of SiNO continuous fiber reinforced BN wave-transparent composites

Cao F., Fang Z., Chen F., Shen Q., Zhang C.

Materials Science Poland

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2012

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Vol. 30, No. 2

137--142

EN

SiNO continuous fiber reinforced boron nitride (BN) wave-transparent composites (SiNOf /BN) have been fabricated by a precursor infiltration pyrolysis (PIP) method using borazine as the precursor. The densification behavior, microstructures, mechanical properties, and dielectric properties of the composites have been investigated. After four PIP cycles, the density of the composites had increased from 1.1 g-cm-3 to 1.81 g-cm-3. A flexural strength of 128.9 MPa and an elastic modulus of 23.5 GPa were achieved. The obtained composites have relatively high density and the fracture faces show distinct fiber pull-out and interface de-bonding features. The dielectric properties of the SiNOf /BN composites, including the dielectric constant of 3.61 and the dielectric loss angle tangent of 5.7-0-3, are excellent for application as wave-transparent materials.

4

Soil seed banks in lakeshore wetlands : relation to the extant vegetation

Peng Y., Wu N., Gao X., Fang Z., Xiao W.

Polish Journal of Ecology

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2010

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Vol. 58, nr 3

449-457

EN

Seed bank composition and germination characteristics are necessary for modeling wetland vegetation composition. Yet there are few studies about the seed bank difference between plant communities in lakeshore wetlands. Seed banks are also known to play important roles in the vegetation restoration process. Environmental factors such as water level, temperature, or nutrient levels can affect vegetation composition and seed bank composition. The relationships between environmental factors and seed banks of wetlands in the field are still unknown. The Jiuzhaigou Nature Reserve is located at the eastern edge of Qinghai-Xizang Plateau. The lakeshore wetland vegetation is dominated by Phragmites australis (Cav.)Trin. ex Steud., Typha latifolia L., Carex pamirensis C. B. Clarke, Equisetum fluviatile L. The wetlands in Jiuzhaigou (118 lakes) are under strong pressure such as trampling and enhanced construction for travel. Plant restoration is necessary for protection of these areas. We investigated the soil seed bank in six lakeshore wetlands in this area using the seedling emergence method. Sediment samples (0-2 cm, 2-5 cm, 5-10 cm intervals from surface to bottom) were taken with core (diameter 5 cm) in April and from each replicate samples were spread on two plastic trays. Trays were randomly arranged in the greenhouse and watered daily. Seedlings were counted weekly after emergence, and removed as soon as they could be identified. Our study showed that seed density in all soil layers samples was negatively correlated to water depth. Water depth can explain 45% variance of species richness in surface layer in sediment. Species richness in extant vegetation can explain 45, 48, 25% variance of species richness in total 10 cm and in 2-5 cm and 5-10 cm layer sediment respectively. Dominant species cover in extant vegetation, site altitude, total nitrogen, total phosphorus and total organic carbon in soil showed no correlation with species richness in seed bank. Mean seed densities in wetlands ranged from 0 to 15945 m[^-2]. A total of 23 species germinated in seed bank, while 85 species were found in extant vegetation. The dominant species in seed bank and extant vegetation showed great difference. The total number of species and seedlings that germinated in different layers was not significantly different. But the second layer had the greatest seed density. The relationship between seed bank and extant vegetation differed from one species to another. We should use different restoration strategies for different plant communities.

5

Study on an improved grey dynamic programming model

Shi H., Liu S., Fang Z., Du H.

Systems Science

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2009

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Vol. 35, no 1

75-79

EN

The purpose of our study is to present an improved grey dynamic programming model. Considering the uncertainty of information, the conception of grey dynamic programming is proposed in this paper. It also studies the programming model when the profit values are interval numbers and figure out the optimal solution by defining the superiority, inferiority and equipollence position degree. A case study is stated in the end to prove the applicability of the model. Our approach consists in treating profit value as positive interval grey number and we get the optimal strategy solution by means of defining standard interval grey number. As a result, an improved grey dynamic programming model is put forward. The algorithms of interval grey numbers and grey algebraic system of interval grey number should be improved further. The model is useful to realize optimal control in the process of uncertain manufacture such as production plan, storage, source distribution, investment and sorting problems. An algorithm based on position degree for the grey dynamic programming model is put forward.

6

Phylogenetic diversity of bacteria and Archaea associated with the marine sponge Pachychalina sp.

Cheng N., Fang Z., Huang H., Fang Z., Wu X., Bao S.

Polish Journal of Ecology

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2008

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Vol. 56, nr 3

505-510

EN

Molecular techniques were employed to document the microbial diversity associated with the marine sponge Pachychalina sp. from South China Sea in March 2003. Using the total microbial DNA as template, bacterial and archaeal 16S rDNAs were amplified by PCR with universal primers. Amplified products were cloned, sequenced and secondarily amplified by PCR. Then the secondarily amplified products were purified to be further characterized by amplified rDNA restriction analysis (ARDRA). According to the enzyme restriction mapping, the apparent difference among them were disclosed. 22 bacterial cloned partial sequences were acquired and most of them were related to proteobacterium. Also, 7 archaeal cloned partial sequences were acquired and a phylogenetic tree was built up.Result shows the prolific bacterial and archaeal diversity of marine sponge Pachychalina sp.