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Analysis of gallic acid and ellagic acid in leaves of Elaeagnus angustifolia L. from different habitats and times in Xinjiang by HPLC with cluster analysis

Sun Yun, Liu Jiajia, Bayertai, Tang Shasha, Zhou Xiaoying

Acta Chromatographica

2021

Vol. 33, no. 2

195--201

Objective: To determine the levels of gallic acid and ellagic acid by using high-performance liquid chromatography (HPLC) with R software hierarchical cluster analysisin Elaeagnus angustifolia L. gathered from different locations in Xinjiang. Methods: A chromatographic column Diamonsil C18 with a size of 4.63250 mm and 5 mm was used with methanol as A and 0.1% phosphoric acid aqueous solution as B as the mobile phase. The flow rate was 1 mL/min for the gradient elution and the injection volume was 5 mL. HPLC was performed with a detection wavelength of 260 nm and chromatographic column of 35 8C. In addition, R software hierarchical clustering method was used for studying the levels of gallic acid and ellagic acid in E. angustifolia L. from 10 areas. Results: Gallic acid and ellagic acid showed a good linear relationship between 7.375 and 236 mg/mL with a correlation coefficient of 0.9999, and between 3.625 and 116 mg/mL with a correlation coefficient of 0.9999 respectively. The average recovery values were 103.98 and 101.57%, and the Relative Standard Deviation (RSD) values were 1.92 and 1.47%. Conclusion: Differences in the levels of gallic acid and ellagic acid in E. angustifolia L. leaves from different areas in Xinjiang showed that both were the highest in Kuitun.