Przedstawiono wyniki badania składu chemicznego i hydrolizy enzymatycznej przeprowadzonej dla słomy kukurydzianej poddanej obróbce wstępnej metodą LHW w różnych temperaturach. Uzyskane wyniki pozwoliły stwierdzić, że wraz z rosnącą temperaturą procesu wzrastała zawartość otrzymywanej w hydrolizie enzymatycznej glukozy. Z kolei w przypadku ksylozy jej zawartość stopniowo malała. Stwierdzono również, że proces obróbki wstępnej metodą LHW miał korzystny wpływ na skład chemiczny słomy kukurydzianej jako potencjalnego substratu do produkcji bioetanolu.
EN
The shredded corn stover (chip size 0.43-1.0 mm) was treated with liq. hot H2O at 160-205°C and then treated with a com. enzyme in an aq. environment. The glucose and xylose contents in the hydrolyzates were detd. with high performance liq. chromatog. An increase of temp. in the thermal treatment resulted in an increase in glucose and decrease in xylose contents.
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The aim of this work was to present a short review of studies concerning corn stover chemical composition. Analysis of corn stover composition was focused on its elemental and chemical substances composition. Impact of many different factors determining the discrepancies in obtained results was discussed. The importance of corn stover studying as a potential raw material for the production of bioethanol was underlined. Various methods of pretreatment have been mentioned, with particular emphasis on biological methods, which are cheap and ecological.
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A beneficial influence of the steam explosion pretreatment on simple sugars yield after enzymatic hydrolysis was observed. The highest average glucose content (25.8%) in the hydrolysate after steam explosion pretreatment at 190 oC was achieved. In turn, an application of steam explosion pretreatment at 205 oC resulted in a decrease of enzymatic hydrolysis efficiency and 21.8% of glucose was obtained only. In case of xylose, a similar correlations were observed. The highest average xylose content (4.0%) at 190 oC was obtained and the decrease of xylose content (to the level of 2.4%) after enzymatic hydrolysis of biomass pretreated at 205 oC was observed. Probably, the decrease of the sugars content was caused by a condensation reactions of lignin and polysaccharides. Other explanation of the obtained results may be formation of inhibitors, which could hinder enzymatic hydrolysis. This reason may be especially important, because in these studies to enzymatic hydrolysis process unwashed solid fraction was used. Obtained results were correlated to the chemical composition of the studied wood. The partial hemicelluloses degradation and dissolution led to decrease in its content and at the same time increased the content of extractives. Lignin content stayed roughly the same for both untreated material and steam explosion pretreated at 160 oC and 175 oC. However, its content increased rapidly after steam explosion pretreatment at 190 oC and 205 oC. Cellulose content was not a subject to significant changes, although its apparent increase, when compared to cellulose content in untreated wood, was probably due to decline in hemicelluloses content.
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