Wyniki wyszukiwania - BazTech

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Molekularne sondy fluorescencyjne w biologii, biochemii i biotechnologii

Kukuła K., Kamińska I., Ortyl J., Chachaj-Brekiesz A.

Technical Issues

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2015

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nr 4

19--25

EN

Nowadays molecular fluorescent probes are widely used in many fields of science, primarily in biology, medicine and biotechnology. Particular emphasis on the development of technologies to cancer cells diagnosis and detection of genetic mutations led to numerous modification of luminescent fluorophores, which has resulted in a wide range of such compounds. Scientists from around the world are continuously trying to experience the mechanism of cells function and metabolic pathways. Certainly, it is affected by the fact that any analytical techniques using fluorescence operate easily and nondestructively. A phenomenon of fluorescence is observable by chromophore which absorbs energy at a certain wavelength and is able to emit electromagnetic radiation at another wavelength.

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Próba oceny kierunków i tempa zmian infrastruktury transportowej w krajach nowoprzyjętych i aspirujących do UE

Kukuła K., Strojny J.

Badania Operacyjne i Decyzje

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2006

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nr 3-4

99-112

PL

Przedstawiono zróżnicowanie poziomu i kierunki rozwoju infrastruktury transportowej w nowych krajach członkowskich UE oraz w państwach aspirujących do tej organizacji. Rozważa się również tempo i kierunki transformacji badanego zjawiska. Zastosowanie metody statystycznej ma na celu dokonanie charakterystyki wielowymiarowego problemu poprzez utworzenie zmiennej agregowanej. Metodologia tworzenia tego miernika obejmuje proces normalizacji bazowych charakterystyk (unitaryzacja zerowana). Rezultatem badania jest dokonanie typologii badanych krajów ze względu na poziom rozwoju studiowanego problemu. Nastepnie wykonano prognozę kierunków rozwoju infrastruktury w poszczególnych krajach. Obejmuje ona oszacowanie czasowe wyrównywania dysproporcji rozwojowych.

EN

The paper presents the diversity of the transport infrastructure development in new EU members and countries aspiring to that organisation. Directions and the tempo of this phenomenon transformation are considered, as well. A statistical analysis is employed to characterise multivariate phenomenon by creation of an aggregate variable. The methodology of building up this measure encompasses normalisation process of diagnostic characteristics (zeroed unitarisation). The outcome of the research is a typology of countries according to the developmental level of an issue under investigation. Subsequently the research focuses on the prognosis of directions of the infrastructure transformation in individual countries. The forecast includes the time estimate of developmental disproportions equalization.

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Stream habitat conditions and fish fauna within the occurrence range of Wałecki barbel, Barbus cyclolepis waleckii Rolik, 1970 (Teleostei: Cyprinidae) in Polish part of the Carpathian Mts

Amirowicz A., Kukuła K.

Polish Journal of Ecology

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2005

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Vol. 53, nr 4

503-522

EN

Existing data on Wałecki barbel are very scarce and need a complement to the quantitative description of its habitat conditions. This fish is a subspecies of the Pontic species B. cyclolepis Heckel which occurs in some coastal rivers of the Black Sea or, as evidenced by some results of studies on mitochondrial DNA, it is a hybrid originating from natural crossing between the common barbel Barbus barbus (L.) and spotted barbel B. carpathicus Kotlik, Tsigenopoulos, Rab et Berrebi. Until now the Wałecki barbel was reported from 26 sites in the river basins of the Upper Vistula and the Dniester in SE Poland (49°09'-49°49' N, 21°28'-22°50' E, altitude 200-590 m). Seventeen of these sites were studied in autumn 2002 and summer 2003 to collect data on habitat parameters of stream channels. The main characteristics of their morphology (i.e. length, width, depth, and gradient) were calculated on the basis of a set of field measurements. Current velocity was measured using float method. The composition of bottom substratum was estimated using a simple scale based on Wentworth scale. Additionally, two biotic characters were included, i.e. the occurrence of macroscopic aquatic vegetation and the species composition of fish community. According to the obtained results the habitat conditions appropriate for Wałecki barbel may be defined as follows: 1) The stream should be medium-sized, at least 10-m or better 20-m wide. 2) The mean channel depth should exceed 20 cm, while the maximum depth should be greater than 50 cm. The channel morphology with the zone of medium depth (i.e. that extending over the half of bottom) in range between at least 15 and 40 cm seems to be favorable. 3) The mean stream velocity of 0.30 m s-1 or more is desired. It seems that the zone of fast current (0.50-1.00 m s-1) sharing a considerable portion of the channel is an important habitat feature. 4) Most of the bottom area should be covered by medium-sized particles (2-200 mm) with the mode within range of fine pebbles (20.50 mm). 5) In general, the stream should be rather oligotrophic than eutrophic with poorly developed macroscopic algae and the absence of vascular plants, and with flow fluctuations periodically disturbing structure of bottom substratum. 6) The abundant occurrence of spotted barbel, especially within lower part of its distribution area and in the zone of its co-occurrence with common barbel may be regarded as a good indicator of the stream habitat appropriate for Wałecki barbel. 7) Other frequent and abundant species accompanying Wałecki barbel are minnow Phoxinus phoxinus (L.), stone loach Barbatula barbatula (L.), chub Leuciscus cephalus (L.) and schneider Alburnoides bipunctatus (Bloch).

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Skład chemiczny wody potoku Wołosaty i jego dopływów

Granops M., Kukuła K., Płużański A.

Zeszyty Naukowe Politechniki Rzeszowskiej. Budownictwo i Inżynieria Środowiska

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2000

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z. 32[180], cz.2

87-92

EN

The paper presents water chemistry of the Wołosaty river and the tributaries in 1983-85 and 1995-1997 in the National Park of Bieszczady.