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1
Content available Influence of enzymatic pretreatment on yield and chemical composition of Rosmarinus officinalis essential oil
Dzięcioł Małgorzata
Polish Journal of Chemical Technology
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2022
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Vol. 24, nr 4
61--66
EN
Effect of enzymatic pretreatment before hydrodistillation process on yield and composition of Rosmarinus officinalis essential oil was studied. Results obtained by using two selected commercial enzymes applied in food and beverage industry were compared. Control process with non-enzymatic pretreatment in analogous conditions was also performed for proper interpretation of results. Application of gas chromatography with mass selective detector (GC-MS) enabled analysis and comparison of essential oils composition. Moreover, total phenolic content (TPC) was determined spectrophotometrically in post-processing hydrolates, which are also valuable products e.g. for cosmetic applications. Modifications of isolation process by pretreatment with selected enzymes resulted in significant increase in essential oil yields in comparison to conventional hydrodistillation and control process with non-enzymatic pretreatment in analogous conditions. No substantial changes in the composition of obtained essential oils were observed. In post-processing hydrolates higher values of total phenolic content (TPC) were found both after enzymatic and non-enzymatic pretreatment.
2
Content available Effect of chemical modification of hydrodistillation on yield, composition and biological activity of Rosmarinus officinalis essential oil
Dzięcioł Małgorzata
Polish Journal of Chemical Technology
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2021
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Vol. 23, nr 3
49--53
EN
Studies on the chemical modifications of Rosmarinus officinalis essential oil hydrodistillation process (HD) by using 5% citric acid (CA-HD) and 5% trisodium citrate (TSC-HD) ase water phase were performed. Composition of essential oils obtained in conventional and modified conditions was analyzed by gas chromatography with mass selective detector method (GC-MS) and compared. Antioxidant activity of all essential oils was determined spectrophotometrically by using DPPH radical scavenging method. It was found that applied modifications of hydrodistillation process enhanced yields and antioxidant activity and the best results were obtained using 5% citric acid as a modifier. Effect of this modification on fungicidal activity of essential oils against 8 various fungi strains (Alternaria alternata, Botrytis cinerea, Fusarium culmorum, Phythophtora cactorum, Rhizoctonia solani, Phythophtora infestans, Sclerotinia sclerotiorum and Ascosphaera apis) was also determined and in most cases enhanced activity was observed.
3
Content available Influence of extraction technique on yield and antioxidant activity of extracts from Moringa oleifera leaf
Dzięcioł Małgorzata
Polish Journal of Chemical Technology
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2020
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Vol. 22, nr 4
31--35
EN
The article presents research on the exploring of extraction process of biologically active substances from the leaves of the Moringa oleifera tree using ethanol. Ethanolic extracts were obtained using three different techniques: maceration with shaking, ultrasound-assisted extraction and extraction in Soxhlet apparatus, in different time variants: 1, 2 and 4-hours. After solvent evaporating and drying, the yields of dry extracts obtained in particular processes were calculated. The antioxidant activity of extracts was analyzed spectrophotometrically using DPPH radical scavenging method, and total phenolic content (TPC) was determined by Folin-Ciocalteu method. By means of gas chromatography with mass selective detector (GC-MS), 11 biologically active compounds present in ethanolic extracts were identified, among which α-tocopherol had the greatest share. Based on the results, the influence of the extraction technique and time on the yield and antioxidant activity of M. oleifera leaf extracts were discussed.
4
Content available Comparison of the composition and fungicidal activity of essential oils from fennel fruits cultivated in Poland and Egypt
Wodnicka Alicja, Huzar Elżbieta, Dzięcioł Małgorzata, Krawczyk Maria
Polish Journal of Chemical Technology
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2019
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Vol. 21, nr 2
38--42
EN
The yield, composition and fungicidal activity of essential oils obtained from fennel fruits cultivated in Poland (FEOPOL) and Egypt (FEO-EG) were compared. The influence of the duration of hydrodistillation using a Clevenger apparatus on the essential oil yield was studied. The composition of the fennel essential oils was determined by GC-MS method. Studies have shown that FEO-POL and FEO-EG are two distinct chemotypes, which differ in yield and composition. The fennel fruits cultivated in Poland contained 4.14% of essential oil with trans-anethole as a main component. The plant material from Egypt was characterised by low content of essential oil (1.32%) with a predominant share of estragole. The fungicidal activity was tested in vitro against ten species of pathogenic fungi. The best result for FEO-POL was achieved against Sclerotinia sclerotiorum, Rhizoctonia solani and Botrytis cinerea. Antifungal activity of FEO-EG against tested fungi was weak or none.
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