Wyniki wyszukiwania - BazTech

1

Zmiany rozkładu przestrzennego widm dyfrakcyjnych kolonii bakterii wywołanych działaniem wybranych czynników fizykochemicznych

Linard M., Buzalewicz I., Ulatowska-Jarża A.

Acta Bio-Optica et Informatica Medica. Inżynieria Biomedyczna

|

2017

|

Vol. 23, nr 1

1--12

PL

Problemy identyfikacji bakterii oraz zwalczania zakażeń bakteryjnych należą do jednych z najważniejszych zagadnień współczesnej nauki w kontekście przeciwdziałania wzrastającej lekooporności bakterii. Istnieje zatem potrzeba opracowania nowych, szybszych i bardziej powszechnych metod detekcji oraz identyfikacji bakterii, środków i czynników bakteriobójczych, jak również technik pozwalających na charakteryzację efektywności ich działania. W niniejszej publikacji zbadano korelację pomiędzy zmianami rozkładów przestrzennych widm dyfrakcyjnych Fresnela kolonii bakterii wywołanych działaniem wybranych czynników fizykochemicznych: niskiej temperatury, alkoholi oraz promieniowania UV. Przeprowadzona analiza jakościowa oraz ilościowa uzyskanych wyników wykazała bezpośredni związek pomiędzy zmianami rozkładu przestrzennego widm dyfrakcyjnych kolonii a działaniem czynników hamujących rozwój kolonii bakterii. Uzyskane wyniki świadczą o możliwości wykorzystania analizy widm dyfrakcyjnych kolonii bakterii do oceny efektywności działania materiałów, środków chemicznych i czynników fizycznych o działaniu antybakteryjnym.

EN

The problem of bacteria identification and limitation of the bacteria contamination risk are the most important issues of contemporary science in the context of increasing bacterial drug resistance. Therefore, there is a need to develop new, faster, and more common methods of bacteria detection and identification, antibacterial agents and factors, as well as techniques to characterize their effectiveness against bacteria. In this paper the correlation between the spatial changes of bacterial colonies Fresnel diffraction patterns and the colonies treatment by selected physicochemical factors: low temperature, alcohols, and UV radiation was investigated. The performed qualitative and quantitative analysis of obtained results confirmed that there is a direct relation between the bacterial colonies diffraction patterns spatial changes and the factors influencing bacterial colonies growth. Obtained preliminary results demonstrated the possibility of the application of the diffraction patterns analysis to characterize the efficiency of different physicochemical factors with antibacterial activity.

2

Photoactivated titania-based nanomaterials for potential application as cardiovascular stent coatings

Kopaczyńska M., Sobieszczańska B., Ulatowska-Jarża A., Hołowacz I., Buzalewicz I., Wasyluk Ł., Tofail S. A. M., Biały D., Wawrzyńska M., Podbielska H.

Biocybernetics and Biomedical Engineering

|

2014

|

Vol. 34, no. 3

189--197

EN

Intravascular stenting of atherosclerotic coronary arteries is a life-saving, widely used procedure in interventional cardiology. Adverse clinical outcomes such as restenosis high-light the importance of meeting the excellent biocompatibility by cardiovascular implants. Many attempts have been made to improve the safety profile of implant surface. We for the first time developed the photoactive intravascular titania-based nanomaterials for the application as cardiovascular stent coating. Photoactive biomaterial deposited on the cardiovascular stent surface demonstrated promising features, making it an excellent substrate for endothelial cells growth and proliferation. The biocompatibility of these coatings has been compared with 316L stainless steel surfaces typically used in commercial coronary stents production. The results of the study proved that the innovative titania- based coatings have better biocompatibility characteristics than the 316L stainless steel and in regard of its antithrombotic potential provided protection against restenosis. Further-more, the titania coating supported endothelial cells attachment and proliferation, and induced prolonged plasma recalcification time in comparison with stainless steel surface. Innovative photoactive titania coating can be an important factor to prevent the process of the restenosis in the place of implantation.

3

Wykorzystanie metody VELOXY do dezynfekcji małej aparatury medycznej – doniesienie wstępne

Suder M., Ulatowska-Jarża A

Acta Bio-Optica et Informatica Medica. Inżynieria Biomedyczna

|

2013

|

Vol. 19, nr 3

133--139

PL

W niniejszej pracy opisano wykorzystanie metody VELOXY (VEry Low OXYgen – metoda dezynfekcji przy niskim stężeniu tlenu), stosowanej zazwyczaj do ochrony dóbr dziedzictwa kulturowego, do dezynfekcji małej aparatury medycznej. Potencjalnie metoda ta mogłaby pozwolić na skuteczne zabezpieczenie antymikrobowe aparatury medycznej, takiej jak np. glukometry i inne drobne aparaty medyczne, wypożyczane czasowo pacjentom. Zaletą tej metody jest długoterminowe i bezproblemowe przechowywanie zabezpieczonej mikrobiologicznie aparatury medycznej. Przeprowadzone badania miały na celu ilościowe określenie wpływu różnych środków dezynfekcyjnych na zabezpieczenie antymikrobowe glukometrów.

EN

In this paper, the VELOXY method (VEry Low OXYgen – method based on the extremely low oxygen concentration), usually used for the protection of cultural heritage, was applied for the disinfection of small medical equipment. Potentially, this method may be exploited for antimicrobial protection of the small medical appliances, temporarily available for the patient, as for example glucometers. The advantage of this method may be long-term microbiologically secure storage of medical equipment. The aim of the presented study was to quantify the antimicrobial effects of various disinfectants.

4

Sol-gel coated fiberoptic applicator for photodynamic medicine - optical and AFM characterization

Kopaczyńska M., Hołowacz I., Ulatowska-Jarża A., Buzalewicz I., Podbielska H.

Biocybernetics and Biomedical Engineering

|

2012

|

Vol. 32, no. 1

41-50

EN

The application of spectroscopic study, microscopic and AFM imaging for examination of fiberoptic applicators is presented. The potential carriers of photoactive agents for photodynamic medicine in form of sol-gel coatings of fiberoptic applicators, are proposed. Optical and morphological properties of the proposed sol-gel coatings doped with photosensitizer Photolon, are characterized. The influence of pH and oxygen changes on entrapped Photolon properties, was examined, as well. The morphology of the applicator coating was examined by using atomic force microscopy. The light distribution from an applicator was studied by means of computer aided image analysis.

5

Badanie fluorescencji światłouczulacza fotofonu w komórkach He-La

Czernielewski L., Przybyło M., Ulatowska-Jarża A., Podbielska H.

Acta Bio-Optica et Informatica Medica. Inżynieria Biomedyczna

|

2012

|

Vol. 18, nr 1

55-58

PL

W pracy badano wnikanie światłouczulacza fotolonu do komórek He-La. Przeprowadzono badania spektroskopowe fotolonu w obecności i bez formaldehydu, stosowanego do utrwalania komórek. Badania mikroskopowe przeprowadzono za pomocą mikroskopu konfokalnego. Komórki inkubowano ze światłouczulaczem przez 4 i 12 godzin. Natężenie fluorescencji fotolonu w komórkach zależy od stężenia roztworu i czasu inkubacji. W wyniku przeprowadzonych pomiarów mikroskopowych wykazano, że czas inkubacji niezbędny do zaobserwowania związku wewnątrz komórek wynosi co najmniej 4 godziny, a minimalne stężenie światłouczulacza 1 mg/ml (1,67 × 10-6 M).

EN

The entrapment of the photosensitizer Photolon into the He-La cells, was studied. The fluorescence of photosensitizer was examined without and in the presence of formaldehyde. The study was performed by means of confocal microscopy of cells incubated for 4 hours and 12 hours. It was stated that 4 hours is the shortest time of incubation allowing the observation of Photolon in the He-La cells and the minimal concentration of photosensitizer is 1mg/ml (1,67 × 10-6 M).

6

Nanotechnology for biomedical applications - enhancement of photodynamic activity by nanomaterials

Ulatowska-Jarża A., Pucińska J., Wysocka-Król K., Hołowacz I., Podbielska H.

Bulletin of the Polish Academy of Sciences. Technical Sciences

|

2011

|

Vol. 59, nr 3

253-261

EN

Over the last two decades nanotechnology has become one of the most dynamically evolving field of research. The unique properties of nanomaterials, not disclosing at microscale, are examined and exploited to extend our understanding of the interactions taking place at atomic or molecular level. Those findings affect research in many areas, like e.g. alternative energy sources, electronics, physics and medicine. In this paper, the possibility of using nanomaterials for the enhancement of photodynamic activity, is discussed. A brief review on drug-delivery facilitating nanomaterials, regarding their characteristic features, is presented. An exemplary application of silver-doped nanomaterials for enhancement of photodynamic properties of two photosensitizers: Photolon and Protoporphyrin IX, is described. Influence of silver-doped nanomaterials addition on the fluorescence intensity of photosensitizers immobilized in silica-titania (SiO2-TiO2) sol was examined via VIS spectroscopy. Influence of sonication on the fluorescence enhancement was also investigated. It was demonstrated that the fluorescence enhancement of photosensitizers depends on the concentration of both: photosensitizer and silver-doped nanoparticles.

7

Silica-based versus silica-titania sol-gel materials - comparison of the physical properties: surface tension, gelation time, refractive index and optical transmittance

Ulatowska-Jarża A., Hołowacz I., Wysocka K., Podbielska H.

Optica Applicata

|

2009

|

Vol. 39, nr 1

211--220

EN

Knowledge of the physical parameters and optical constants of sols and sol-gel materials is important in view of their possible applications. In this work, silica and silica-titania sols were examined. Gelation time, surface tension, refractive index and optical transmittance were measured. The sol-gel materials were produced from the silica precursor Si(OC2H5)4 mixed with the titanium dioxide precursor Ti(OC2H5)4 and 96% ethyl alcohol. It was stated that the surface tension of pure silica sols is much higher than in case of silica-titania sols. The refractive index of fresh samples is lower than of stored sols. The transmittance is highest for the fresh samples and it decreases with gelation time; however, this effect is stronger for silica-titania materials. By manipulating with the solvent content and titania addition, the required refractive index may be obtained.

8

Fluorescence properties of sol-gel materials doped with photosensitizers

Hołowacz I., Ulatowska-Jarża A., Wysocka K., Głuchowski W., Stręk W., Podbielska H.

Optica Applicata

|

2008

|

Vol. 38, nr 1

49-56

EN

Sol-gel materials may found plenty of applications, e.g., as carriers for various substances, which can be exploited for sensing purposes or as drug releasing carriers. In this work, the fluorescence properties of two photosensitizers immobilized in silica based sol-gel materials were examined. The sol-gel materials were produced from silica precursor TEOS and 96% ethyl alcohol as solvent. Materials with various ratios R (solvent to precursor molar ratio) 5, 15, 20, 32, 40, and 50, were prepared. Photolon and protoporphyrine PPIX were used as dopants in concentrations 0.001%, 0.005%, 0.01% and 0.05%. The excitation wavelength from a blue laser was 415 nm. The highest luminescence was observed for the material prepared with R = 20, whereas for R = 5 it was the lowest one. The fluorescence quenching for highest photosensitizer concentration was observed, as well.

9

Porównanie antybakteryjnej aktywności fotodynamicznej in vitro protoporfiryny i fotolonu

Ulatowska-Jarża A., Borkowska J., Kościelniak K., Hołowacz I., Wieliczko A., Stręk W., Podbielska H.

Acta Bio-Optica et Informatica Medica. Inżynieria Biomedyczna

|

2007

|

Vol. 13, nr 4

361-367

PL

Celem pracy było zbadanie wrażliwości bakterii Salmonella enteritidis i Staphylococcus aureus na oddziaływanie foto-dynamiczne in vitro przy zastosowaniu dwóch różnych fo-touczulaczy: protoporfiryny PPIX i fotolonu w stężeniach 0,5%, 3,5%, 5,2%, 10%. Bakterie gram-ujemne (Salmonella enteritidis) są bardziej czule na promieniowanie laserowe o długości fali 662 nm niż bakterie gram-dodatnie (Staphylococcus aureus). Aktywność fotodynamiczna zależy od stężenia fotouczulacza i czasu naświetlania. Fotolon w wyższych stężeniach działa słabiej niż w niższych, natomiast protoporfiryna w wyższych stężeniach działa bardziej foto-cytotoksycznie.

EN

The aim of this work was to examine the in vitro sensitivity of Salmonella Enteritidis and Staphylococcus aureus to photodynamic activity of Protoporphyrine PPIX and fotolon in various concentrations: 0,5%, 3,5%, 5,2%, 10%. The Gram- bacteria (Salmonella Enteritidis) were more sensitive to 662nm laser radiation than Gram-I- bacteria (Staphylococcus aureus). Photodynamic activity depends on the concentration of photosensitizer and exposure time. Fotolon in higher concentration exhibits weaker photodynamic activity, whereas Protoporphyrine in higher concentrations is more photocytotoxic.

10

Viscosity, surface tension and refractive index of tetraethylorthosilicate-based sol-gel materials depending on ethanol content

Hołowacz I., Podbielska H., Bauer J., Ulatowska-Jarża A.

Optica Applicata

|

2005

|

Vol. 35, nr 4

691-699

EN

The sol-gel-derived materials can be exploited for the number of applications, including biomaterials and measuring techniques. One of the most important applications involves production of sol-gel coatings. In this study, silica based sol-gel materials were prepared by way of acid hydrolysis with alcohol as a solvent. Tetraethylorthosilicate (TEOS) was used as a precursor. Different samples were tested; with molar ratios R = 5, 10, 15, 20, 32 and 50, denoting the number of ethanol moles to the number of TEOS moles. The viscosity and surface tension of liquid hydrolizates were measured depending on the ethanol content in sol-gel. The thickness of a coating layer was determined, as well. The refractive index was measured 10 days long, up to the point of gelation. It was demonstrated that viscosity, surface tension and refractive index are lower for higher R value (for higher alcohol content), whereas the sol-gel layers produced with higher R values are thicker than these ones produced with lower alcohol content. It is also demonstrated that R value influences the performance of fiberoptic sol-gel applicators for interstitial laser therapy.

11

Influence of heating temperature on structural properties of sol-gel materials

Bauer J., Ulatowska-Jarża A., Podbielska H., Lechna-Marczyńska M., Bindig U., Muller G., Beuthan J.

Optica Applicata

|

2005

|

Vol. 35, nr 4

791-797

EN

In this paper, the influence of heating temperature on the properties of sol-gel materials was investigated . The aim of the study was to examine the production repeatability. All samples were made with factor R = 15, denoting the ratio of the number of precursor (TEOS) moles to solvent (water) moles. The samples were prepared in acid hydrolysis route on water, whereas two groups were considered: in one group the hydrolysis was terminated by addition of NH3źH2O and in the other there was no addition of base. The samples were stored for 1 month at room temperature and then exposed to high temperatures: 1000, 1200 and 1350°C. They were examined by means of scanning electron microscopy (SEM). The electron microphotographs were recorded, digitized and analyzed. The Fisher linear discriminant analysis for evaluation of the microscopic images was exploited. It was shown that the repeatability varied between 83.24% and 95.48%. The analysis demonstrated that the best results were achieved for neutralized samples made at 1000°C.

12

Spectroscopic properties of a chlorophyll-based photosensitive dye entrapped in sol-gel fibre-optic applicators

Ulatowska-Jarża A., Bindig U., Podbielska H., Hołowacz I., Stręk W., Müller G., Eichler H. J.

Materials Science Poland

|

2005

|

Vol. 23, No. 1

111--122

EN

One of the promising modalities of modern medical treatment is interstitial laser therapy, where a special fibre-optic applicator is used to ensure a proper curing light distribution in the pathological lesion. Such an applicator can act as a light diffuser, and simultaneously it can serve as a carrier of the therapeutic medium, e.g. a photosensitive dye for photodynamic therapy in situ. For applicator construction, silica based sol-gels coatings are proposed in this paper. The sol-gel applicators were prepared from the silicate precursor TEOS (tetraethylorthosilicate) mixed with ethyl alcohol in acid-catalysed hydrolysis. A suitable amount of surfactant (Triton X-100) was used. The carrier matrices were produced with a solvent to precursor molar ratio of 20. In these studies, optical fibres from Laser Components were used (core diameter 400 nm, HCS, low OH). The external jacket was mechanically removed at a distance of 25 mm. The modified dip-coating method was exploited to cover the bare fibres with sol-gel material. Two types of applicators were produced, silica sol-gels with an addition of chlorophyll-derived sensibilisator (Photolon) in two various concentrations. It was proved that the immobilization of Photolon in a silica sol-gel does not destroy its chemical activity and does not disturb contact with the external environment.

13

Repeatability of sol-gel biocoatings on optical fibers examined by statistical pattern recognition methodology

Bauer J., Podbielska H., Ulatowska-Jarża A., Holowacz I., Müller G., Beuthan J.

Inżynieria Biomateriałów

|

2005

|

R. 8, nr 47-53

227--228

14

Sol-gel technology for biomedical engineering

Podbielska H., Ulatowska-Jarża A.

Bulletin of the Polish Academy of Sciences. Technical Sciences

|

2005

|

Vol. 53, nr 3

261-271

EN

Sol-gel derived silica possess many promising features, including low-temperature preparation procedure, porosity, chemical and physical stability. Applications exploiting porous materials to encapsulate sensor molecules, enzymes and many other compounds, are developing rapidly. In this paper some potential applications, with emphasis on biomedical and environmental ones, are reviewed. The material preparation procedure is described and practical remarks on silica-based sol-gels are included. It is reported that sol-gels with entrapped various molecules may be used in construction of implants and coatings with bioactive properties. It is shown how to exploit the sol-gel production route for construction of sol-gel coated fiberoptic applicators for lasertherapy. The applications of bioactive materials are discussed, as well. 11 is demonstrated that it is possible to immobilize photosensitive compounds in sol-gel matrix without loosing their photoactivity. Some examples of sol-gel based biosensors are demonstrated, as well, showing their potential for detecting various gases, toxic substances, acidity, humidity, enzymes and biologically active agents.

15

Termowizyjna ocena światłowodowych aplikatorów zol-żelowych do interstycjalnej termoterapii laserowej

Hołowacz I., Bindig U., Liebold K., Podbielska H., Ulatowska-Jarża A., Muller G., Scheller E.

Acta Bio-Optica et Informatica Medica. Inżynieria Biomedyczna

|

2005

|

Vol. 11, nr 3-4

14-18

PL

W pracy badano możliwości koagulacyjne aplikatorów zol-żelowych wykonanych na bazie TEOS-u (tetraethyloksysilan) zmieszanego z alkoholem etylowym w procesie hydrolizy kwaśnej. Zol nałożono w postaci ośmiowarstwowego filmu na odpłaszczony rdzeń światłowodu. Wykonano 3 rodzaje aplikatorów o długości 1 cm, 2 cm i 3 cm. Wszystkie aplikatory przetestowano w układzie z kamerą termowizyjną AGEMA 900 i rejestrowano rozkład temperatury w aplikatorze podłączonym za pomocą włókna światłowodowego do lasera półprzewodnikowego Ceralas D, emitującego promieniowanie o długości fali 980 nm i mocy maksymalnej 25 W. Na podstawie termogramów wyznaczono maksymalną temperaturę w zależności od mocy wyjściowej lasera i długości aplikatora. Wykazano, że maksymalne gęstości mocy na 1 cm długości aktywnej aplikatorów (1 cm, 2 cm, 3 cm) wynosiły odpowiednio: 8,73 W/cm, 4,75 W/cm, 2,75 W/cm. Przeprowadzono również badania koagulacji białka jaja kurzego. Wykazano, że objętość skoagulowanego obszaru zależy od mocy promieniowania laserowego. Pokazano również, że na tę objętość ma wpływ pozycja aplikatora. Aplikator umieszczony poziomo w odległości 1 cm od powierzchni białka w naczyniu, z powodu szybszego chłodzenia, prowadził do uzyskania mniejszego obszaru koagulacji (5 cm2), o kształcie spłaszczonego dysku w stosunku do aplikatora umieszczonego pionowo w naczyniu - 11 cm2 w kształcie elipsoidy obrotowej, dla tych samych warunków eksperymentu: 20 minut ekspozycji na promieniowanie lasera 20 W.

EN

The sol-gel based applicators coagulation performance was tested. The applicators were made from TEOS (tetraethyloksysilan) mixed with ethanol in acid hydrolysis. The sol was deposited on fiberoptic core, after removing of fiberoptic clad. The fiber was placed in the container with hole In bottom and the liquid flowed out, leaving the coating on the core. This procedure was repeated 8 times, thus the 8-layers coating was produced. Three types of applicators were prepared of various lengths: 1 cm, 2 cm and 3 cm. The Ceralas laser, emitting 980 nm wavelength with maximal 25 W output power was used In the experiment. AGEMA 900 thermovision camera registered the thermograms of applicators depending on the output power of the laser and applicator length. The maximal temperature was determined from thermograms. It was found that the maximal power densities on the unit length of applicators were 8,73 W/cm, 4,75 W/cm, 2,75 W/cm, for 1 cm, 2 cm and 3 cm long applicators, respectively. The coagulation experiments were performed, as well. As a medium white of hen eggs was used. It was shown that the coagulated volume depends on powerl density and the position of applicator. For applicator placed vertically in the container with egg white the maximal coagulated volume was 11 cm2 and had a form of ellipsoid. In case of applicator placed horizontally (parallel to the egg white surface, 1 cm beneath) the coagulated volume was 5 cm2 and had a form of flattened disc. In the same experimental condition: 20 minute exposure, 20W output power. It was caused by more rapid cooling of the white surface in the second case.

16

Photosensitizers for PDT entrapped in sol-gel applicators

Ulatowska-Jarża A., Bindig U., Podbielska H., Hołowacz I., Müller G., Eichler H., Stręk W.

Acta Bio-Optica et Informatica Medica. Inżynieria Biomedyczna

|

2004

|

Vol. 10, nr 1-2

29-29

17

Mutual correlation of sol-gel optical properties and repeatability of production process examined by statistical pattern recognition methods

Kobel J., Podbielska H., Lechna-Marczyńska M., Ulatowska-Jarża A.

Optica Applicata

|

2003

|

Vol. 33, nr 1

97-105

EN

The methods based on the statistical pattern recognition are used for the evaluation of microscopic images of sol-gel matrices. The applied classification is a two steps scheme: dimensionality reduction of the data and cluster analysis. The obtained results have demonstrated that the molar ratio R (the number of solvent moles to the number of substrate moles) influences the stability of sol-gel materials, thus enabling us to reach the higher repeatability of the production process. It has been shown that the repeatability varies between 96.67% (for molar ratio R = 5) and 98.33% (for R = 15, 32, 50). This corresponds with the results of light scattering and transmission measurements.

18

EPR studies of defects in pure sol-gel matrices and their influence on cytotoxicity of the material

Ulatowska-Jarża A., Komorowska M., Podbielska H.

Materials Science

|

2003

|

Vol. 21, No. 4

487-494

EN

Sol-gel based biomaterials may be used for various applications, including biomedical ones. In his respect it is important to investigate the influence of sol-gel matrices on biological systems in order to establish their cytotoxic activity. The results of EPR studies of sol-gels are described in this work. They demonstrate that various defects are present in sol-gel matrices: surface defects, peroxy- centres or hydrogen-related centres. These defects arise spontaneously during the gelation, being responsible for cytotoxicity of sol-gels. It was found that the ratio of the number of solvent moles to the number of prekursor moles (the molar ratio R) is one of the factors determining the level of cytotoxicity. More defects were present in samples prepared with a lower molar ratio R. A higher concentration of defects was detected in freshly prepared samples, as compared to samples aged for a longer time.

19

Influence of sample preparation methods on transmission electron micrographs of sol-gel materials

Jeleń Ł., Cegielski M., Ulatowska-Jarża A., Podbielska H.

Optica Applicata

|

2002

|

Vol. 32, nr 4

759-766

EN

The main goal of this research was to examine the influence of a sample preparation method on microscopic images obtained in transmission electron microscopy (TEM). The microscopic images of porous silica-based sol-gel materials were further processed with computer-aided methods, so thus to obtain information about distribution of pores. As a stain dopant osmium tetraoxide was used. Sol-gels were prepared from TEOS (tetraethyl orthosilicate) as a precursor and water as a solvent. The material was placed on microscopic grids, pure and with cellulose, or it was embedded in resin and then cut on a microtome. It has been demonstrated that the preparation procedure influences the pores distribution in a sol-gel silica network examined by TEM.

20

Biosensor for urea detection based on sol-gel technology

Ulatowska-Jarża A., Podbielska H.

Optica Applicata

|

2002

|

Vol. 32, nr 4

685-690

EN

The sol-gel technology allows for the preparation of porous glass-like materials, which can be promising hosts for various organic molecules. In this paper the entrapment of urease in a sol-gel matrix is described. Since urease is an enzyme catalyzing the reaction of urea, it can be used for urea detection. The sol-gel bulk with immobilized urease serves as an optode of the urea fiber optics sensing system. It has been proved that urease encapsulated in the sol-gel matrix does not change its chemical activity The decomposition of urea causes an increase in pH. The changes in pH are measured by monitoring the changes in absorption of a pH indicator. In this study, the bromothymol blue was used. The results of measurements with the enzymatic sol-gel optode are presented.